Document Type : Short Communication
Authors
- Pouria Motamed Chaboki 1
- Arash Ghalyanchilangeroudi 1
- Vahid Karimi 2
- Hamed Abdollahi 3
- Hossein Maghsoudloo 3
- Hossein Hosseini 4
- Reza Khaltababdi Farahahni 3
- Seyed Ali Ghafouri 3
- Mohammad Hossein Falah 5
- Hussein Rezaee 1
1 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2 Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Iranian Veterinary Organization, Tehran, Iran
4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran
5 Razi Vaccine and Serum Research Institute , Tehran ,Iran
Abstract
Avianmetapneumovirus (aMPV), also known as avian pneumovirus or turkey rhinotracheitis virus, is the causative agent of turkey rhinotracheitis and swollen head syndrome in chickens. Four aMPV subgroups (A-D) have been reported previously based on their genetic and antigenic differences. Evidence suggests that the live bird markets (LBMs) play an important role in the epidemiology of the avian viral diseases. A total number of 450 oropharyngeal samples from eight different species of birds (migratory and local) were collected from LBMs of Gilan province, Iran, from October to December 2016. The presence of aMPV was determined by reverse transcription polymerase chain reaction (RT-PCR) based on nucleoprotein gene. The aMPV was detected in 30.60% of the examined birds including chickens (37.00%), turkey (33.00%), Eurasian teal (25.00%), common blackbird (33.00%), and Eurasian woodcock (25.00%). Bioinformatics analysis and a phylogenetic tree based on partial nucleotide sequences of the N gene showed that the detected aMPVs were belonged to subtype B. This is the first report of aMPV in non-commercial birds in Iran. Knowledge of the frequency and types of infected birds with pneumoviruses allow a better understanding of the epidemiology of aMPV in Iran.
Keywords
Main Subjects
- Owoade A, Ducatez M, Hübschen J, et al. Avian metapneumovirus subtype A in China and subtypes A and B in Nigeria. Avian Dis 2008; 52(3):502-506.
- Banet-Noach C, Simanov L, Perk S. Characterization of Israeli avian metapneumovirus strains in turkeys and chickens. Avian Pathol 2005;34(3):220-226.
- Hosseini H, Ghalyanchi-Langeroudi A. Detection and molecular characterization of avian metapneumovirus in Iran: The first report. Iran J Virol 2012;6(2):26-31.
- Al-Shekaili T, Baylis M, Ganapathy K. Molecular detection of infectious bronchitis and avian metapneumoviruses in Oman backyard poultry. Res Vet Sci 2015;99:46-52.
- Garmsar I. Phylogenetic study based on the gene of attachment protein (G) avian metapneumovirus from broiler breeder farm in Iran, 2013. Iran J Virol 2013;7(3):7-11.
- Bäyon-Auboyer MH, Arnauld C, Toquin D, et al. Nucleotide sequences of the F, L and G protein genes of two non-A/non-B avian pneumoviruses reveal a novel APV subgroup. J Gen Virol 2000;81(11):2723-2733.
- Tamura K, Peterson D, Peterson N, et al. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28(10):2731-2739.
- Seal BS, Wise MG, Pedersen JC, et al. Genomic sequences of low-virulence avian paramyxovirus-1 (Newcastle disease virus) isolates obtained from live-bird markets in North America not related to commonly utilized commercial vaccine strains. Vet Microbiol 2005; 106(1):7-16.
- Rahimi M. Seroprevalence of avian metapneumovirus infection in broiler and broiler breeder chickens in Iran. Vet Med. 2011; 56:395-399.
- Homayounfar N, Shoushtari H, Charkhkar S, et al. Detection by reverse transcriptase-polymerase chain reaction and molecular characterization of avian metapneumovirus in chicken flocks in Iran. Walia J 2015; 31(S3):170-174.
- Shin HJ, Njenga MK, McComb B, et al. Avian pneumovirus (APV) RNA from wild and sentinel birds in the United States has genetic homology with RNA from APV isolates from domestic turkeys. J Clin Microbiol 2000; 38(11):4282-4284.
- Bennett R, Nezworski J, Velayudhan B, et al. Evidence of avian pneumovirus spread beyond Minnesota among wild and domestic birds in central North America. Avian Dis 2004; 48(4):902-908.
- Bennett R, McComb B, Shin H, et al. Detection of avian pneumovirus in wild Canada geese (Branta canadensis) and blue-winged teal (Anas discors). Avian Dis 2002;46(4):1025-1029.
- Turpin E, Stallknecht D, Slemons R, et al. Evidence of avian metapneumovirus subtype C infection of wild birds in Georgia, South Carolina, Arkansas and Ohio, USA. Avian Pathol 2008;37(3):343-351.
- Cha RM, Yu Q, Zsak L. The pathogenicity of avian metapneumovirus subtype C wild bird isolates in domestic turkeys. Virol J 2013;10(1):38. doi: 10.1186/1743-422X-10-38.
- Felippe PA, Silva LH, Santos MB, et al. Detection of and phylogenetic studies with avian metapneumovirus recovered from feral pigeons and wild birds in Brazil. Avian Pathol 2011;40(5):445-452.
- Catelli E, Cecchinato M, Savage CE, et al. Demonstration of loss of attenuation and extended field persistence of a live avian metapneumovirus vaccine. Vaccine 2006;24(42):6476-6482.
- Cecchinato M, Catelli E, Lupini C, et al. Avian metapneumovirus (AMPV) attachment protein involvement in probable virus evolution concurrent with mass live vaccine introduction. Vet Microbiol 2010;146(1):24-34.
)