Veterinary Research Forum
Vet Res Forum

Production of a monoclonal antibody against chicken immunoglobulin G: A valuable molecule with research and diagnostic applications

Document Type : Original Article

Authors

Masoud Reza Seyfi Abad Shapouri 1
Mohammad Rashno 2
Pezhman Mahmoodi 3
Mahshid Ariya 4

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Department of Pathobiology, Faculty of Veterinary Science, Bu-Ali Sina University, Hamedan, Iran

4 Graduate student, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, , Ahvaz, Iran

Abstract
Monoclonal antibodies (MAbs) are invaluable molecules which have several advantages over polyclonal immunoglobulins (Igs) including consistency and higher specificity and hence can be used in biological researches, diagnosis and treatment of diseases. The present study was conducted to produce monoclonal antibody against chicken IgG.TheIgG molecules were purified from chicken serum and used as antigens to immunize several mice. Thereafter, a well-immunized mouse was chosen and used for fusion process. After production of hybridoma cells, several rounds of cloning were carried out and produced MAbs were examined by various immunological assays including enzyme-linked immunosorbent assay (ELISA) and western and dot blotting. Assessment of grown hybridomas indicated that only one clone (5B8) has produced desired MAb against chicken IgG. Meanwhile, using an indirect ELISA, it was shown that this MAb successfully recognizes chicken IgG molecules attached to influenza virus nucleoprotein. Evaluation of cross reactivity of MAb 5B8 with several avian serum samples revealed that this molecule specifically identifies chicken antibody molecules. However, it also recognized turkey antibodies with less affinity. In addition to research applications like isolation and purification of chicken and turkey IgG molecules, such a MAb can be applied to design and development of various immunoassays (e.g. ELISA) in these avian species.

Keywords

Chicken
ELISA
Immunoglobulin ‎G
monoclonal antibody

Subjects

  1.  

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Veterinary Research Forum
Volume 9, Issue 1
Winter 2018
Pages 67-72

  • Receive Date 28 May 2017
  • Revise Date 20 October 2017
  • Accept Date 24 October 2017

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