Veterinary Research Forum
Vet Res Forum

ECOR phylotyping and determination of virulence genes in Escherichia coli isolates from pathological conditions of broiler chickens in poultry slaughter-houses of southeast of Iran

Document Type : Original Article

Authors

Akbar Asadi 1
Taghi Zahraei Salehi 2
Mahmoud Jamshidian 1
Reza Ghanbarpour 3

1 Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Molecular Microbiology Research Group, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

https://doi.org/10.30466/vrf.2018.30827
Abstract
Avian pathogenic Escherichia coli (APEC) are responsible for wide ranges of extra-intestinal diseases in poultry including colibacillosis, cellulitis, coligranuloma and yolk sac infection. Numbers of virulence are considered important in the pathogenicity of these diseases. The aims of the present study were phylogenetic typing and virulence genes detection in Escherichia coli isolates from colibacillosis and cellulitis of broiler chickens in poultry slaughterhouses of Shahrbabak region, Kerman, Iran. A total number of eighty three E. coli isolates were taken from broiler chickens with colibacillosis and thirty four isolates were taken from carcasses with cellulitis in the industrial slaughterhouses. Biochemically confirmed E. coli isolates were subjected to polymerase chain reaction assay to determine phylogenetic groups and presence of pap C, sfa/focDE, iucD, afaIB-C, hlyA, fimH and crl virulence genes. Colibacillosis isolates were belonged to A (54.21%), B1 (7.22%), B2 (6.03%) and D (32.53%) phylogroups. Whereas, the isolates from cellulitis cases were belonged to three main phylogroups; A (55.88%), B1 (5.88%) and D (38.24%). Statistical analysis showed a specific association between the presence of crl virulence gene and phylogroups of A and D in colibacillosis isolates. The results showed that the isolates from both diseases in broiler chickens could be assigned to various phylogenetic groups (mainly A(. Also, the virulence genes profile of cellulitis E. coli is completely different from that of colibacillosis in this region.

Keywords

Cellulitis
Colibacillosis
Escherichia coli
Phylogenetic group
Virulence genes

Subjects

  1.  

    1. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2010; 66: 4555-4558.
    2. Saif YM, Fadly AM, Glisson JR, et al. Diseases of poultry. 12th ed. London, UK: Blackwell 2008;121-123.
    3. Johnson JR, Murray AC, Gajewski A, et al. Isolation and molecular characterization of nalidixic acid-resistant extra-intestinal pathogenic Escherichia coli from retail chicken products. Antimicrob Agents Chemother 2003; 47: 2161-2168.
    4. Roy P, Purushothaman V, Koteeswaran A, et al. Isolation, characterization, and antimicrobial drug resistance pattern of Escherichia coli isolated from Japanese quail and their environment. J Appl Poultry Res 2006; 15(3): 442-446.
    5. de Brito BG, Gaziri LC, Vidotto MC. Virulence factors and clonal relationships among Escherichia coli isolates isolated from broiler chickens with cellulitis. Infect Immun 2003; 71:4175-4177.
    6. Ghanbarpour R, Salehi M, Oswald E. Virulence genotyping of Escherichia coli isolates from avian cellulitis in relation to phylogeny. Comp Clin Pathol 2010; 19:147-153.
    7. de Campos TA, Stehling EG, Ferreira A, et al. Adhesion properties, fimbrial expression and PCR detection of adhesin-related genes of avian Escherichia coli isolates. Vet Microbiol 2005; 106(3): 275-285.
    8. Kawano M, Yaguchi K, Osawa R. Genotypic analyses of Escherichia coli isolated from chickens with colibacillosis and apparently healthy chickens in Japan. Microbiol Immunol 2006; 50: 961-966.
    9. Jafari RM, Motamedi H, Maleki E, et al. Phylogenetic typing and detection of extended-spectrum β-lactamases in Escherichia coli isolates from broiler chickens in Ahvaz, Iran. Vet Res Forum 2016; 7(3): 227-233.
    10. Mohsenifard E, Asasi K, Sharifiyazdi H, et al. Phylotyping and ColV plasmid-associated virulence genotyping of E. coli isolated from broiler chickens with colibacillosis in Iran. Com Clin Patho 2016; 25: 1035-1042.
    11. Ghanbarpour R, Askari N, Ghorbanpour M, et al. Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran. Trop Anim Health Prod 2017; 49(3):591-597.
    12. Alizadeh H, Ghanbarpour R, Jajarmi M, et al. Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail. Vet Res Forum 2017; 8(1):55-58.
    13. Yamamoto S, Terai A, Yuri K, et al. Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol Med Microbiol 1995; 12(2): 85-90.
    14. Van Bost S, Jacquemin E, Oswald E, et al. Multiplex PCRs for identification of necrotoxigenic Escherichia coli. J Clin Microbiol 2003; 41:4480-4482.
    15. Dissanayake DR, Wijeuardana TG, Gunawardena G, et al. Distribution of lipopolysaccharide core type among avian pathogenic E. coli in relation to the major phylogenetic groups. Vet Microbiol 2008; 132(3-4):355-363.
    16. Ewers C, Li G, Wilking H, et al. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: How closely related are they? Int J Med Microbiol 2007; 297: 163-176.
    17. Mellata M. Human and avian extra-intestinal pathogenic Escherichia coli: Infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog Dis 2013; 10(11): 916-932.
    18. Mora A, Viso S, López C, et al. Poultry as reservoir for extra-intestinal pathogenic Escherichia coli O45:K1:H7-B2-ST95 in humans. Vet Microbiol 2013; 167(3-4): 506-512.
    19. Li G, Laturnus C, Ewers C, et al. Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infect Immun 2005; 73: 2818-2827.
    20. La Ragione RM, Woodward MJ. Virulence factors of Escherichia coli serotypes associated with avian colisepticaemia. Res Vet Sci 2002; 73: 27-35.
    21. Mokady D, Gophna U, Ron EZ. Virulence factors of septicemic Escherichia coli isolates. Int J Med Microbiol 2005; 295(6-7); 455-462.
    22. Delicato ER, de Brito BG, Gaziri LC, et al. Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis. Vet Microbiol 2003; 94: 97-103.
    23. Dozois CM, Fairbrother JM, Harel J, et al. pap- and pil-related DNA sequences and other virulence determinants associated with Escherichia coli isolated from septicemic chickens and turkeys. Infect Immun 1992; 60: 2648-2656.
    24. Jeffrey JS, Nolan LK, Tonooka KH, et al. Virulence factors of Escherichia coli from cellulitis or colisepticemia lesions in chickens. Avian Dis 2002; 46(1): 48-52.
Veterinary Research Forum
Volume 9, Issue 3
Summer 2018
Pages 211-216

  • Receive Date 18 April 2018

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us