Morphometric development of sheep (Ovis aries) lymph nodes in fetal period

Document Type : Original Article

Authors

1 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Food hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 DVM Graduated from Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Immune system plays crucial role in body and lymph nodes are essential parts of this system for combating pathogens. However, no study has ever been conducted on morphometric development of sheep lymph nodes in fetal period. Thus, this study attempted to examine the morphometric characteristics of a number of important lymph nodes of some lymphocenters of sheep during fetal period. To this end, 60 pieces of sheep fetuses collected from Ahvaz slaughterhouse were fixated in 10% formalin and then divided into four categories based on crown-rump length (CRL) following gender and weight determinations. Mandibular, caudal superficial cervical (prescapular), caudal mediastinum, jejunal mesenteric and popliteal lymph nodes were evaluated in five lymphocenters of head, neck, thoracic cavity, abdominal viscera and pelvic limbs, respectively. In each sample, nodes formation was visually checked and in cases of nodes formation, they were measured in terms of weight, length, width and thickness and collected data were statistically analyzed. The longest and shortest fetal CRLs were found to be 48.50 cm and 3.50 cm, respectively. Gender had no effect on study parameters in 32 male and 28 female fetuses. Study of sheep fetuses’ lymph nodes revealed no macroscopic lymph node development by day 45, while all nodes were observable after the day 59. The shortest lymph node was mandibular node and the longest one was caudal mediastinum. Based on the results, it seemed that although the size of lymph nodes grows by age, this increase is not the same for all nodes and groups.

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Main Subjects


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    1. Schoenwolf GC, Bleyl SB, Brauer PR, et al. Larson's human embryology. 5th ed. Philadelphia, USA: Churchill Livingstone 2015: 334.
    2. McGeady TA, Quinn PJ, Fitzpatrick ES, et al. Veterinary embryology. UK: John Wiley & Sons 2017; 341-342.
    3. Schummer A, Wilkens H, Vollmerhus B, et al. The circulatory system, the skin and the cutaneous organs of the domestic mammals. Berlin: Germany: Verlag Paul Parey 1981; 278-294.
    4. Konig HE, Liebich HG. Veterinary anatomy of domestic mammals. 1st ed. Schattauer, Munich, Germany: Schattauer 2007; 452-453.
    5. Getty R. Sisson and Grossman's the anatomy of the domestic animals. 5th ed. New York, USA: WB Saunders 1975; 176-179.
    6. Dyce KM, Sack WO, Wensing CJG. Textbook of veterinary anatomy. 3rd ed. Philadelphia, USA: WB Saunders 2010; 29-30.
    7. Bagi AS, Vyas KN, Bhayani DM. Study on the various dimensions of superficial regional lymph nodes in young and adult Surti buffalo (Bubalus bubalis). Indian Vet J 1992; 69(2): 172-175.
    8. Panchal KM, Vyas KN, Vyas YL. Histomorphological study on secondary lymphoid organs (spleen, sub-lumbar lymph nodes and Peyer's patches) of Marwari sheep (Ovis aries). Indian Vet J 1982; 75(4): 318-322.
    9. Yoon YS, Shin JW, Lee JS. Age related morphological studies on hemal node and hemolymph node in Korean native goats. Korean J Vet Res 1999; 39(5): 865-877.
    10. Asha A, Maya S, Harshan KR, et al. Morphometric observation on lymph nodes in goat foetuses. J Vet Anim Sci 2004; 42: 30-33.
    11. Pospieszny N, Kleckowska J, Chroszcz A, et al. The morphology and development of the sheeps tracheobronchial cranial lymph nodes in prenatal period. Vet Med 2002; 5(2): #02.
    12. Maddox JF, Makay CR, Brandon MR. Ontogeny of ovine lymphocytes an immunohistological study on the development of T lymphocytes in the sheep fetal spleen. Immunology 1987; 62(1): 107-112.
    13. Zöltzer H. Initial lymphatics: Morphology and function of endothelial cells. Lymphology 2003; 36(1): 7-25.
    14. Noakes DE, Parkinson TJ, England GCW. Arthur’s veterinary reproduction and obstetrics. London, UK: WB Saunders 2001; 68.
    15. Hyttel P, Sinowatz F, Vejlted M. Essentials of domestic animal embryology. London, UK: Sanders 2010; 237.
    16. Mohammadpour AA, Radmehr B, Al-e-Ahmad Dehkordi M. Morphological and morphometrical study of important lymph nodes of cow in meat inspection [Persian]. Pajouhesh-va-Sazandegi 2006; 71: 13-18.
    17. Mohammadpour AA, Moshtaghi H, Naderi AR. Morphometry and histometrical study of prescapular, prefemoral and popliteal lymph nodes in Iranian Lori-Bakhtiari sheep [Persian]. Pajouhesh-va-Sazandegi 2010; 86: 47-52.
    18. Raji R, Feizbakhsh H. Morphometrical study of prescapular, prefemoral and popliteal lymph node of one-humped camel (Camelus dromedaries) [Persian]. Pajouhesh-va-Sazandegi 2010; 87: 47-52.
    19. Dabanoglu I, Baradakcioglu HE, Sevil F, et al. Morphometry of the lymph node excluding the thoracic and abdominal cavity in Wistar rats. Turk J Vet Anim Sci 2004; 28: 775-778.
    20. Gadre KM, Malik MR, Srivastava AM. Gross anatomical changes in abdominal lymph node cross bred calves with age and immune response. Indian J Anim Sci 1986; 56: 623-633.
    21. Sarma K, Kalita A, Suri S, et al. Gross anatomical observations on the superficial lymph nodes of Bakarwali goat (Capra hircus). Indian J Anim Sci 2004; 74: 750-751.