Veterinary Research Forum
Vet Res Forum

Evaluation of intravitreal injection of pentoxifylline in experimental endotoxin-induced uveitis in rabbits

Document Type : Original Article

Authors

Mohammad Reza Khalili 1
Amin Hossein Amini 2
Mohammad Abbaszadeh Hasiri 2
Effat Baghaei Moghaddam 2
Masoomeh Eghtedari 1
Mohammad Azizzadeh 3
Mousa Zare 1
Masood Yasemi 1

1 Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

https://doi.org/10.30466/vrf.2018.32083
Abstract
The objective of the present study was to investigate the clinical and histopathological effects of intravitreal injection of pentoxifylline (PTX) the management of an experimental model of uveitis. Fifty-two rabbits were divided randomly into six intravitreal treated groups as below: 1) Balanced salt solution (BSS), 2) Salmonella typhimurium lipopolysaccharide endotoxin (LPS) + BSS, 3) LPS + PTX 100 μg, 4) LPS + PTX 500 μg, 5) BSS + PTX 100 μg and 6) BSS + PTX 500 μg. Inflammation was evaluated by clinical examinations using slit lamp on days 1, 3, 5 and 7 post injections and histopathological examinations were also performed at the end of the study. Clinical examinations demonstrated a statistically significant difference between group 1 and group 2 on day 5 and day 7. Moreover, the comparison of clinical severity scores of group 1 with groups 3, 4, 5 and 6, on third, fifth and seventh post-injection days showed statistically significant differences. The mean histopathological inflammation intensity score in groups 5 and 6 was significantly higher than group 1. The mean histopathological inflammation intensity score in groups 3, 4, 5 and 6 was significantly higher than group 2. Intravitreal injection of PTX in an experimental model of uveitis in rabbits not only does not reduce inflammation but also leads to inflammation when used alone or in combination with LPS.

Keywords

Endotoxin-induced uveitis
Intravitreal injection
Pentoxifylline
Tumor necrosis factor-α

Subjects

  1.  

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Veterinary Research Forum
Volume 9, Issue 3
Summer 2018
Pages 239-244

  • Receive Date 15 August 2018

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