This study was conducted to evaluate the effect of subcutaneous administration of synthetic eugenol (EG) for disbudding of goat kids, as a new chemical method. Thirty apparently healthy Raieni (Cashmere) goat kids (five-day-old) were divided randomly into six groups (n = 5). In the pathology (P) groups (P3, P8 and P60 according to the sample collection day after injection) an amount of 0.10 mL of EG was subcutaneously administrated in both horn buds. In the disbudding 1 and 2 (DB1, DB2) groups, 0.10 mL of EG, and in the control (C) group 0.10 mL normal saline was subcutaneously injected in the right horn buds, respectively. Eugenol injection in DB2 group was done in twelve-day-old goat kids. The left horn buds of DB1, DB2 and C groups were considered as control of horn outgrowing. The horn buds, kidneys, liver, lung, brain and heart, tissue specimens were collected from P3 and P8 groups, and bud skin samples were collected from P60 group. The results showed that the EG was able to stop the horn growth in the first week of goat life. There was no significant difference between left and right horn size in the C group. Histopathological study revealed complete necrosis of bud tissue in dermal and epidermal layers, in P3 animals. Healing and re-epithelialization were seen in the samples taken from P8 group. Subcutaneous injection of the synthetic EG can be considered as a new method for goat kids disbudding.