Veterinary Research Forum
Vet Res Forum

Chemical disbudding of goat kids with subcutaneous administration of synthetic eugenol: Histopathology and morphometry

Document Type : Original Article

Authors

Mohammad Farajli Abbasi 1
Mohammad Mahdi Molaei 1
Reza Kheirandish 2
Ali Mostafavi 3

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Chemistry, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran

https://doi.org/10.30466/vrf.2018.32084
Abstract
This study was conducted to evaluate the effect of subcutaneous administration of synthetic eugenol (EG) for disbudding of goat kids, as a new chemical method. Thirty apparently healthy Raieni (Cashmere) goat kids (five-day-old) were divided randomly into six groups (n = 5). In the pathology (P) groups (P3, P8 and P60 according to the sample collection day after injection) an amount of 0.10 mL of EG was subcutaneously administrated in both horn buds. In the disbudding 1 and 2 (DB1, DB2) groups, 0.10 mL of EG, and in the control (C) group 0.10 mL normal saline was subcutaneously injected in the right horn buds, respectively. Eugenol injection in DB2 group was done in twelve-day-old goat kids. The left horn buds of DB1, DB2 and C groups were considered as control of horn outgrowing. The horn buds, kidneys, liver, lung, brain and heart, tissue specimens were collected from P3 and P8 groups, and bud skin samples were collected from P60 group. The results showed that the EG was able to stop the horn growth in the first week of goat life. There was no significant difference between left and right horn size in the C group. Histopathological study revealed complete necrosis of bud tissue in dermal and epidermal layers, in P3 animals. Healing and re-epithelialization were seen in the samples taken from P8 group. Subcutaneous injection of the synthetic EG can be considered as a new method for goat kids disbudding.

Keywords

Disbudding
Goat kid
Histopathology
Radiology
Synthetic eugenol

Subjects

  1.  

    1. Dyce KM, Sack WO, Wensing CJG. Textbook of Veterinary Anatomy-E-Book. Edinburgh, UK: Elsevier Health Sciences 2009;355-360.
    2. Al-Sobayil FA. A new simple device for dehorning in small ruminants. Small Rumin Res 2007; 67(2-3):232-234.
    3. Oehme FW. Textbook of large animal surgery. Baltimore, USA: Williams and Wilkins 1988;180-185.
    4. Fubini SL, Ducharme NG. Textbook of farm animal surgery: St. Louis, USA: Saunders; 2004;511-515.
    5. Koger LM. Dehorning by injection of calcium chloride. Vet Med Small Anim Clin 1976; 71(6):824-825.
    6. Guidotti TL. Critique of available studies on the toxicology of kretek smoke and its constituents by routes of entry involving the respiratory tract. Arch Toxicol 1989; 63(1):7-12.
    7. Kihurani D, Mbiuki S, Ngatia T. Healing of dehorning wounds. Brit Vet J 1989; 145(6):580-585.
    8. Petrie N, Mellor D, Stafford K, et al. Cortisol responses of calves to two methods of disbudding used with or without local anesthetic. New Zeal Vet J 1996; 44(1):9-14.
    9. Bengtsson B, Menzel A, Holtenius P, et al. Cryosurgical dehorning of calves: a preliminary study. Vet Rec 1996; 138(10):234-237.
    10. Molaei MM, Mostafavi A, Kheirandish R, et al. Study of disbudding goat kids following injection of clove oil essence in horn bud region. Vet Res Forum 2015; 6(1):17-22.
    11. Pavithra B. Eugenol-A Review. Indian J Med Res. 1981; 73:443-451.
    12. Carrasco A, Espinoza C, Cardile V, et al. Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I). J Brazil Chem Soc 2008;19(3):543-548.
    13. Alma MH, Ertas M, Nitz S, et al. Chemical composition and content of essential oil from the bud of cultivated Turkish clove (Syzygium aromaticum L.). Bioresources 2007;2(2):265-269.
    14. Myint S, Wan Daud WR, Mohamad AB. Determination of optimal conditions for extraction of alcohol-soluble eugenol containing material from cloves. Pertanika J Sci Technol 1995; 3(1):99-106.
    15. Guan W, Li S, Yan R, et al. Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods. Food Chem 2007; 101(4):1558-1564.
    16. Hernández-Sánchez P, López-Miranda S, Lucas-Abellán C, et al. Complexation of eugenol (EG), as main component of clove oil and as pure compound, with β-and HP-β-CDs. Food Nut Sci 2012; 3(6):716-723.
    17. Ohkubo T, Shibata M. The selective capsaicin antagonist capsazepine abolishes the antinociceptive action of eugenol and guaiacol. J Dent Res 1997; 76(4):848-851.
    18. Jaganathan SK, Supriyanto E. Antiproliferative and molecular mechanism of eugenol-induced apoptosis in cancer cells. Molecules 2012; 17(6):6290-6304.
    19. Pisano M, Pagnan G, Loi M, et al. Antiproliferative and pro-apoptotic activity of eugenol-related biphenyls on malignant melanoma cells. Mol Cancer 2007; 6(1):8. doi:10.1186/1476-4598-6-8.
    20. Kim GC, Choi DS, Lim JS, et al. Caspases-dependent apoptosis in human melanoma cell by eugenol. Korean J Anat 2006; 39(3):245-253.
    21. Pal D, Banerjee S, Mukherjee S, et al. Eugenol restricts DMBA croton oil induced skin carcinogenesis in mice: Downregulation of c-Myc and H-ras, and activation of p53 dependent apoptotic pathway. J Dermatol Sci 2010; 59(1):31-39.
    22. Kurian R, Arulmozhi DK, Veeranjaneyulu A, et al. Effect of eugenol on animal models of nociception. Indian J Pharmacol 2006; 38(5):341-345.
    23. Daniel AN, Sartoretto SM, Schmidt G, et al. Anti-inflammatory and antinociceptive activities A of eugenol essential oil in experimental animal models. Rev Bras Farmacogn 2009; 19(1B):212-217.
    24. Raghavenra H, Diwakr B, Lokesh B, et al. Eugenol:The active principle from cloves inhibits 5-lipoxygenase activity and leukotriene-C4 in human PMNL cells. Prostaglandins Leukot Essent Fatty Acids 2006; 74(1):23-27.
    25. Robbers JE, Tyler VE. Tyler's herbs of choice: The therapeutic use of phytomedicinals. Binghampton, USA: Haworth Press Inc 199;92-98.
    26. Thompson K, Bateman R, Morris P. Cerebral infarction and meningoencephalitis following hot-iron disbudding of goat kids. New Zeal Vet J 2005; 53(5):368-370.
    27. Wright H, Adams D, Trigo F. Meningoencephalitis after hot-iron disbudding of goat kids. Vet Med Small Anim Clin. 1983; 78:599-601.
    28. Sanford SE. Meningoencephalitis caused by thermal disbudding in goat kids. Can Vet J 1989; 30(10):832.
    29. Hull BL. Dehorning the adult goat. Vet Clin N Am Food A 1995; 11(1):183-185.
    30. Buttle H, Mowlem A, Mews A. Disbudding and dehorning of goats. In Pract 1986; 8(2):63-65.
    31. Hague Ba, Hooper R. Cosmetic dehorning in goats. Vet Surg 1997; 26(4):332-334.
    32. Alvarez L, Gutierrez J. A first description of the physiological and behavioural responses to disbudding in goat kids. Anim Welfare 2010; 19(1):55-59.
    33. Hendrickson DA, Baird AN. Text book of Turner and McIlwraith's techniques in large animal surgery. Baltimore, USA: John Wiley & Sons; 2013;293-296.

     

Veterinary Research Forum
Volume 9, Issue 3
Summer 2018
Pages 225-230

  • Receive Date 15 August 2018

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