Veterinary Research Forum
Vet Res Forum

First molecular report of Hydatigera krepkogorski (Schulz and Landa, 1934) in abdominal cavity of gerbil (Rhombomys opimus) in Iran

Document Type : Short Communication

Authors

Eshrat Beigom Kia 1
Mitra Sharbatkhori 2, 3
Zahra Heidari 4
Farideh Tohidi 5
Bahareh Kamran Rashani 1
Farzaneh Zahabiun 1

1 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

2 Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran

3 Department of Medical Parasitology and Mycology, School of Medicine, Golestan University of Medical Sciences, Gorgan, Iran

4 Department of Medical Microbiology and Parasitology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

5 Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran

https://doi.org/10.30466/vrf.2018.33105
Abstract
A polycephalic larva of Taeniidae family isolated from abdominal cavity of a great gerbil (Rhombomys opimus) from Golestan province, northern Iran, was subjected to molecular analysis. Genomic DNA from the larva was obtained using a DNA extraction tissue kit. Polymerase chain reaction was performed for amplification of the partial 12S rRNA, cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase 1 (nad1) mitochondrial genes. BLAST analysis of DNA sequencing indicated 99.00% homology in 12S rRNA and cox1 genes and 98.00% homology in nad1 gene with Hydatigera krepkogorski (accession No. AB731762). The sequences of current isolate were deposited in GenBank by accession Nos. MF281971, MF281972 and MF281973 for 12 SrRNA, cox1 and nad1 genes, respectively. This study was the first report of molecular characterization of H. krepkogorski from Iran. Isolation and characterization of the adult stage from definitive host will help to better clarify incomplete life cycle and morphology data of this species in the world.

Keywords

Hydatigera krepkogorski
Iran
Rhombomys opimus

Subjects

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Veterinary Research Forum
Volume 9, Issue 4
Autumn 2018
Pages 367-371

  • Receive Date 12 November 2018

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