Veterinary Research Forum
Vet Res Forum

Extra intestinal pathogenic Escherichia coli from human and avian origin: Detection of the most common virulence-encoding genes

Document Type : Original Article

Authors

Soheila Najafi 1
Morad Rahimi 2
Zahra Nikousefat 2

1 Student of Veterinary Medicine, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

https://doi.org/10.30466/vrf.2019.34307
Abstract
Pathogenic Escherichia coli strains cause a wide range of extra intestinal infections including urinary tract infection in humans and colibacillosis in poultry. They are classified into uropathogenic E. coli (UPEC) and avian pathogenic E. coli (APEC) with genetic similarities and variations. Their pathogenicity is related to the virulence-encoding genes like sfa, papG II, ompT, iutA, and iss with zoonotic potentials. One hundred isolated E. coli from patients with urinary tract infection and 100 E. coli from chickens with colibacillosis were evaluated for the presence of the most common virulence-encoding genes including sfa, papG II, ompT, iutA, and iss by multiplex polymerase chain reaction. While the frequency of sfa, papG II, ompT, iutA and iss encoding genes in APEC isolates were respectively 0.00%, 67.00%, 63.00%, 89.00% and 89.00%, the frequency of these encoding genes in UPEC isolates were 18.00%, 40.00%, 40.00%, 74.00% and 48.00%, respectively. Except for sfa, the frequencies of other encoding genes in APEC were more than those in UPEC isolates. The iutA as the most common UPEC encoding gene and iss as the most common APEC encoding gene were the most prevalent virulence factors in the examined E. coli isolates. Finding out the distribution of virulence-associated genes could be helpful to identify similarities and differences between APEC and UPEC isolates in order to provide more substantial evidence of their common virulence traits and potential zoonotic threats.

Keywords

Avian colibacillosis
Escherichia coli
Urinary tract infection
Virulence

Subjects

  1.  

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Veterinary Research Forum
Volume 10, Issue 1
Winter 2019
Pages 43-49

  • Receive Date 12 February 2019

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