Veterinary Research Forum
Vet Res Forum

Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs

Document Type : Original Article

Authors

Jamshid Razmyar 1, 2
Mahdis Ghavidel 3
Hamideh Salari Sedigh 2

1 Department of Poultry Diseases, Faculty of veterinary medicine, University of Tehran,Tehran, Iran

2 Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

3 PhD student of Bacteriology, Department of Microbiology, Mashhad University of Medical Sciences, Mashhad, Iran

https://doi.org/10.30466/vrf.2019.34309
Abstract
Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained from dogs by rectal swab. Twenty dogs suffered from diarrhea and 131 of them were healthy. In 9.27% (14/151) of samples, spirochaetes were detected on primary cultures by weak hemolysis and positive Gram staining and then Brachyspira genus was confirmed by NADH oxidase (nox) gene via polymerase chain reaction. Among 14 isolates, twelve isolates were B. canis, one isolate was B. intermedia and another one was non-typeable. From 12 B. canis, only eight isolates were detected by designed specific primers. Ten Brachyspira spp. were isolated from dogs ≤ 1 year old (10/67, 14.92%) and 4 isolates were from > 1 year old dogs (4/84, 4.76%). The isolation rates from healthy and diarrheic dogs were (12/131, 9.16%) and (2/20, 10.00%), respectively. A statistically significant association was observed between the presence of Brachyspira spp. and the age under one year. Based on our findings, the nox gene in B. canis might have more sequence variability compared to other Brachyspira spp.

Keywords

Brachyspiracanis
Dogs
Iran
nox gene
PCR

Subjects

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Veterinary Research Forum
Volume 10, Issue 1
Winter 2019
Pages 67-72

  • Receive Date 12 February 2019

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