Veterinary Research Forum
Vet Res Forum

Detection of multi-antibiotic resistant Campylobacter coli and Campylobacter jejuni in beef, mutton and chicken and water buffalo meat in Ahvaz, Iran

Document Type : Original Article

Authors

Siavash Maktabi 1
Masoud Ghorbanpoor 2
Masomeh Hossaini 3
Amirabbas Motavalibashi 3

1 Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 MSc graduated, Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.30466/vrf.2019.34310
Abstract
Campylobacter jejuni and C. coli are the main causes of gastrointestinal diseases in humans even in industrialized countries affecting public health. The aim of the current study was to evaluate the occurrence and antibiotic resistance of C. jejuni and C. coli in chicken meat, beef, mutton and water buffalo meat slaughtered in Ahvaz city, Iran. A total of 380 samples including chicken meat from industrial abattoirs (n = 150), chicken meat from traditional abattoirs (n = 50), fresh packed chicken meat from local markets (n = 30) and beef, mutton and water buffalo meat from industrial abattoirs (50 samples for each meat) in Ahvaz,were collected and tested for the presence of Campylobacter spp. The procedure was one-step enrichment in Preston enrichment broth followed by plating on supplemented blood agar for 24 hr under microaerophilic conditions at 42 ˚C. Suspected colonies were tested by polymerase chain reaction assay and susceptibility of the confirmed isolates to various antibiotics was investigated by the Kirby-Bauer disk diffusion method. Overall, 32 samples (8.40%) were contaminated with Campylobacter spp. Mutton was the most contaminated meat (24%), while fresh packed chicken meat were not contaminated. Among the 32 isolates, 40.60%, 34.40%, 21.90%, and 15.60% were resistant to tetracycline, ciprofloxacin, ampicillin, and streptomycin, respectively. Moreover, a high number of multi-antibiotic resistant Campylobacter spp. was determined. Since foods of animal origin are the most sources of Campylobacter infection, the presence of resistant strains to antibiotics is a potential risk to public health.

Keywords

Antibiotic resistance
Beef
Campylobacter
Chicken
Mutton
Water buffalo

Subjects

  1.  

    1. Friis L, Pin C, Pearson B, et al. In vitro cell culture methods for investigating Campylobacter invasion mechanisms. J Microbiol Methds 2005; 61(2): 145-160.
    2. Macé S, Haddad N, Zagorec M, et al. Influence of measurement and control of microaerobic gaseous atmospheres in methods for Campylobacter growth studies. Food Microbiol 2015; 52: 169-176.
    3. Brooks GF, Caroll KC, Butel JS, et al. Jawetz, Melnick, & Adelberg's medical microbiology. 25th ed. Columbus, USA: McGraw-Hill Medical. 2006; 342-346
    4. Soltan Dallal M, Sanaei M, Taremi M, et al. Prevalence and antimicrobial resistance pattern of thermophilic Campylobacter spp. (jejuni and coli) isolated from beef and raw chicken in Tehran. J Zanjan Univ Med Sci 2009; 17(68): 85-92.
    5. Liu G, Han Y, Li X, et al. Applicability of a rapid method based on immunomagnetic capture-fluorescent PCR assay for Campylobacter jejuni. Food Control 2006; 17(7): 527-532.
    6. Founou LL, Founou RC, Essack SY. Antibiotic resistance in the food chain: A developing country-perspective. Front Microbiol 2016; 7: 1881.
    7. Khoshbakht R, Tabatabaei M, Hoseinzadeh S, et al. Prevalence and antibiotic resistance profile of thermophilic Campylobacter spp. of slaughtered cattle and sheep in Shiraz, Iran. Vet Res Forum 2016; 7(3): 241-246.
    8. Blaser MJ, LaForce FM, Wilson NA, et al. Reservoirs for human campylobacteriosis. J Infect Dis 1980; 141(5): 665-669.
    9. Stanley K, Jones K. Cattle and sheep farms as reservoirs of Campylobacter. J Appl Microbiol 2003; 94(s1): 104-113.
    10. Hannu T, Mattila L, Rautelin H, et al. Three cases of cardiac complications associated with Campylobacter jejuni infection and review of the literature. Eur J Cli Microbiol Infect Dis 2005; 24(9): 619-622.
    11. Man SM. The clinical importance of emerging Campylobacter species. Nature Rev Gastroenterol Hepatol 2011; 8(12): 669-685.
    12. Jamshidi A, Bassami MR, Farkhondeh T. Isolation and identification of Campylobacter spp. andCampylobacter coli from poultry carcasses by conventional culture method and multiplex PCR in Mashhad, Iran. Iranian J Vet Res 2008; 9(2): 132-137.
    13. Zendehbad B, Arian AA, Alipour A. Identification and antimicrobial resistance of Campylobacter species isolated from poultry meat in Khorasan province, Iran. Food Control 2013; 32(2): 724-727.
    14. Ansari-Lari M, Hosseinzadeh S, Shekarforoush S, et al. Prevalence and risk factors associated with Campylobacter infections in broiler flocks in Shiraz, southern Iran. Int J Food Microbiol 2011; 144(3): 475-479.
    15. Bakhshi B, Kalantar M, Rastegar-Lari A, et al. PFGE genotyping and molecular characterization of Campylobacter spp. isolated from chicken meat. Iran J Vet Res 2016; 17(3): 177-183.
    16. Dabiri H, Aghamohammad S, Goudarzi H, et al. Prevalence and antibiotic susceptibility of Campylobacter species isolated from chicken and beef meat. Int J Enteric Pathog 2014; 2(2): e17087.
    17. Ashrafganjooyi SB, Saedadlei N. Isolation and determine antibiotic susceptibility of Campylobacter jejuni in poultry feces in Kerman. Iran J Medic Microbiol 2016; 9(4): 95-98.
    18. Hakkinen M, Heiska H, Hänninen ML. Prevalence of Campylobacter spp. in cattle in Finland and antimicrobial susceptibilities of bovine Campylobacter jejuni strains. Appl Environ Microbiol 2007; 73(10): 3232-3238.
    19. Granić K, Krčar D, Uhitil S, et al. Determination of Campylobacter spp. in poultry slaughterhouses and poultry meat. Vet Arhiv 2009; 79(5): 491-497.
    20. Whyte P, McGill K, Cowley D, et al. Occurrence of Campylobacter in retail foods in Ireland. Int J Food Microbiol 2004; 95(2), 111-118.
    21. Adwan K. Fast DNA isolation and PCR protocols for detection of methicillin-resistant staphylococci. Folia Microbiol 2014; 59(1): 5-8.
    22. Denis M, Refrégier‐Petton J, Laisney MJ, et al. Campylobacter contamination in French chicken production from farm to consumers. Use of a PCR assay for detection and identification of Campylobacter jejuni and Camp. coli. J Appl Microbiol 2001; 91(2): 255-267.
    23. Wikler MA. Performance standards for antimicrobial susceptibility testing: 16th informational supplement. Wayne, USA: Clinical and Laboratory Standards Institute 2006; 32-38.
    24. Ge B, Wang F, Sjölund-Karlsson M, et al. Antimicrobial resistance in Campylobacter: susceptibility testing methods and resistance trends. J Microbiol Methods 2013; 95(1): 57-67.
    25. Rahimi E, Ameri M, Kazemeini HR. Prevalence and antimicrobial resistance of Campylobacter species isolated from raw camel, beef, lamb, and goat meat in Iran. Foodborne Path Dis 2010; 7(4): 443-447.
    26. Jacobs-Reitsma W, Lyhs U, Wagenaar J. Campylobacter in the food supply. In: Nachamkin I, Szymanski CM, Blaser MJ (Eds). Campylobacter. 3rd ed. Washington, USA: American Society of Microbiology 2008; 627-644.
    27. Kemp R, Leatherbarrow A, Williams N, et al. Prevalence and genetic diversity of Campylobacter spp. in environmental water samples from a 100-square-kilometer predominantly dairy farming area. Appl Environ Microbiol 2005; 71(4): 1876-1882.
    28. Andrzejewska M, Szczepańska B, Śpica D, et al. Trends in the occurrence and characteristics of Campylobacter jejuni and Campylobacter coli isolates from poultry meat in Northern Poland. Food Control 2015; 51: 190-194.
    29. Korsak D, Maćkiw E, Rożynek E, et al. Prevalence of Campylobacter spp. in retail chicken, turkey, pork, and beef meat in Poland between 2009 and 2013. J Food Prot 2015; 78(5): 1024-1028.
    30. Economou V, Zisides N, Gousia P, et al. Prevalence and antimicrobial profile of Campylobacter isolates from free-range and conventional farming chicken meat during a 6-year survey. Food Control 2015; 56: 161-168.
    31. Llarena AK, Sivonen K, Hänninen ML. Campylobacter jejuni prevalence and hygienic quality of retail bovine ground meat in Finland. Let Appl Microbiol 2014; 58(5): 408-413.
    32. Rahimi E, Ameri M, Alimoradi M, et al. Prevalence and antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from raw camel, beef, and water buffalo meat in Iran. Comp Clin Pathol 2013; 22(3): 467-473.
    33. Patrick, M E, Christiansen LE, Wainø M, et al. Effects of climate on incidence of Campylobacter spp. in humans and prevalence in broiler flocks in Denmark. Appl Environ Microbiol 2004; 70(12): 7474-7480.
    34. Guyard-Nicodème M, Rivoal K, Houard E, et al. Prevalence and characterization of Campylobacter jejuni from chicken meat sold in French retail outlets. Int J Food Microbiol 2015; 203: 8-14.
    35. Han X, Zhu D, Lai H, et al. Prevalence, antimicrobial resistance profiling and genetic diversity of Campylobacter jejuni and Campylobacter coli isolated from broilers at slaughter in China. Food Control 2016; 69: 160-170.
    36. Little CL, Richardson JF, Owen RJ, et al. Campylobacter and Salmonella in raw red meats in the United Kingdom: prevalence, characterization and antimicrobial resistance pattern, 2003–2005. Food Microbiol 2008; 25(3): 538-543.
    37. Taremi M, Dallal MMS, Gachkar L, et al. Prevalence and antimicrobial resistance of Campylobacter isolated from retail raw chicken and beef meat, Tehran, Iran. Int J Food Microbiol 2006; 108(3): 401-403.
    38. Van Looveren M, Daube G, De Zutter L, et al. Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium. J Antimicrob Chemother 2001; 48(2): 235-240.
    39. Ge B, White DG, McDermott PF, et al. Antimicrobial-resistant Campylobacter species from retail raw meats. Appl Environ Microbiol 2003; 69(5): 3005-3007.

     

Veterinary Research Forum
Volume 10, Issue 1
Winter 2019
Pages 37-42

  • Receive Date 12 February 2019

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