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Amani, S., Shahrooz, R., Mortaz, E., Hobbenaghi, R., Mohammadi, R., Baradar Khoshfetrat, A. (2019). Histomorphometric and immunohistochemical evaluation of angiogenesis in local ischemia by tissue engineering method in rat: Role of mast cells. Veterinary Research Forum, 10(1), 23-30. doi: 10.30466/vrf.2019.34311
Saeede Amani; Rasoul Shahrooz; Esmaeil Mortaz; Rahim Hobbenaghi; Rahim Mohammadi; Ali Baradar Khoshfetrat. "Histomorphometric and immunohistochemical evaluation of angiogenesis in local ischemia by tissue engineering method in rat: Role of mast cells". Veterinary Research Forum, 10, 1, 2019, 23-30. doi: 10.30466/vrf.2019.34311
Amani, S., Shahrooz, R., Mortaz, E., Hobbenaghi, R., Mohammadi, R., Baradar Khoshfetrat, A. (2019). 'Histomorphometric and immunohistochemical evaluation of angiogenesis in local ischemia by tissue engineering method in rat: Role of mast cells', Veterinary Research Forum, 10(1), pp. 23-30. doi: 10.30466/vrf.2019.34311
Amani, S., Shahrooz, R., Mortaz, E., Hobbenaghi, R., Mohammadi, R., Baradar Khoshfetrat, A. Histomorphometric and immunohistochemical evaluation of angiogenesis in local ischemia by tissue engineering method in rat: Role of mast cells. Veterinary Research Forum, 2019; 10(1): 23-30. doi: 10.30466/vrf.2019.34311

Histomorphometric and immunohistochemical evaluation of angiogenesis in local ischemia by tissue engineering method in rat: Role of mast cells

Article 4, Volume 10, Issue 1, Winter 2019, Page 23-30  XML PDF (1.23 MB)
DOI: 10.30466/vrf.2019.34311
Authors
Saeede Amani1; Rasoul Shahrooz email 1; Esmaeil Mortaz2; Rahim Hobbenaghi3; Rahim Mohammadi4; Ali Baradar Khoshfetrat5
1Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
2Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
4Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
5Department of Chemical Engineering, Sahand University of Technology, Tabriz, Tabriz, Iran
Abstract
The aim of this study was to find a proper method for improvement of ischemic condition in the rat hind limb and also to observe the efficacy of cell engraftment with alginate/gelatin three-dimensional scaffolds. Eighteen male Wistar rats weighing 200 to 250 g were randomly divided into three groups (n = 6) including a) ischemia group; in which femoral artery was removed after ligation at the distance of 5 mm, b) scaffold group; in which hydrogel scaffold was added to the site of transected femoral artery and c) test group; in which in addition to hydrogel scaffold, mast cells (MCs) were also added (1 × 106 cells). Analysis of capillary density, artery diameter, histomorphometric parameters and immunohistochemistry in transected location were done on day 14 after femoral artery transection. The average number of blood capillary was significantly higher in the test group than other groups. Also, the average number of medium and large blood vessels was significantly higher in the test group compared to ischemia and scaffold groups. Application of MCs through the use of hydrogel scaffolds (alginate/gelatin) can be considered as a new approach in the application of stem cells for therapeutic angiogenesis under ischemic conditions which can improve the angiogenesis process in patients with peripheral artery diseases.
Keywords
Angiogenesis; Immunohistochemistry; Mast cell; Rat; Tissue engineering
Main Subjects
Histology
References
 

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