Veterinary Research Forum
Vet Res Forum

The ossification of the vertebral column, thorax and sternum in the quail (Coturnix coturnix japonica)

Document Type : Original Article

Authors

Aris Pourlis 1
John Antonopoulos 2

1 Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, University of Thessaly, Karditsa, Greece

2 Laboratory of Anatomy, Histology and Embryology, School of Veterinary Medicine, Aristotelian University of Thessaloniki, Thessaloniki, Greece

https://doi.org/10.30466/vrf.2019.34312
Abstract
The ossification centers onset of the quail vertebrae, ribs, and sternum in embryos and hatchling birds was studied. Specimens were cleared, stained with Alcian Blue and Alizarin Red S and examined using stereomicroscope. The chondral rudiments of the vertebrae were observed at the 6th day of incubation (E6). The osteogenesis of the vertebrae was accomplished with both perichondral and endochondral ossifications. The cervical vertebrae began to ossify at E9-E10, whereas the thoracic ones began at E10-E11. The synsacral vertebrae began to ossify at E11-E13. In the caudal vertebrae, ossification was observed at E14 and in the pygostylous ones, at E15. The true ribs began to ossify at E7, whereas the 1st and the 2nd ribs began to ossify at E9 and E8, respectively. The uncinate processes were ossified late at E15. At E13, ossification was observed in the caudo-lateral process of the sternum. At E14, the cranio-lateral process of the sternum began to ossify, whereas late at and after hatching ossification was observed in the carina and the sternal body, respectively. The data presented here provide useful baseline information on the normal sequential pattern of ossification in the vertebral column and thoracic cage in quail.

Keywords

Ontogeny
Osteology
Quail
Skeletal development

Subjects

  1. Romanoff AL. The avian embryo: Structural and functionaldevelopment. New York, USA: Mac Millan 1960. 907-1016
  2. Rumpler Y. Chronological appearance of the ossification points of the hen embryo skeleton [French]. C R Assoc Anat 1962; 48: 1175-1191.
  3. Schumacher GH, Wolff E. comparative osteogenesis of Gallus domesticus, Larus ridibundus and Larus canus, I. Chronological appearance of ossifications in Gallus domesticus [German]. Gegenbaurs Morphol Jahrb 1967; 110: 359-373.
  4. Rinaldi L, Caronna EW. The embryo ossification of the chicken (Gallus domesticus L.) [Italian]. Arch Ital Anat Embriol 1971; 76: 201-237.
  5. Scala G, Colella G, Gentile R. Chronology of the appearance of embryonic ossification nuclei in the turkey. Boll Soc Ital Biol Sper 1993; 59(5): 638-644.
  6. Maxwell EE. Comparative embryonic development of the skeleton of the domestic turkey (Meleagris gallopavo) and other galliform birds. Zoology 2008; 111: 242-257.
  7. Atalgin SH, Kürtül I. A morphological study of skeletal development in turkey during the pre-hatching stage. Anat Histol Embryol 2009; 38(1): 23-30.
  8. Shapiro F. Vertebral development of the chick embryo during days 3-1 9 of incubation. J Morphol 1992; 213(3): 317-333.
  9. Heidweiller J. Post natal development of the neck system in the chicken (Gallus domesticus L.). Am J Anat 1989; 186(3): 258-270.
  10. Hogg DA. A re-investigation of the centers of ossification in the avian skeleton at and after hatching. J Anat 1980; 130(Pt 4): 725-743.
  11. Hogg DA. Fusions occurring in the postcranial skeleton of the domestic fowl. J Anat 1982; 135(Pt 3): 501-512.
  12. Ainsworth SJ, Stanley RL Evans DJR. Developmental stages of the Japanese quail. J Anat 2010; 216(1): 3-15.
  13. Starck JM. Evolution of avian ontogenies. Curr Ornithol1993; 10: 275-366.
  14. Mitgutsch C, Wimmer C, Sanchez-Villagra MR, et al. Timing of ossification in duck, quail and zebra finch: Intraspecific variation, heterochronies, and life history evolution. Zoolog Sci 2011; 28(7): 491-500.
  15. Starck JM. Time pattern of ontogeneses in nest-nesting and nest-squatting birds [German]. Stuttgart, Germany: Cour Forsch Inst Senckenberg 1989; 114: 1-319.
  16. Zacchei AM. The embryonic development of the Japanese quail (Coturnix coturnix japonica T. and S.) [Italian]. Archiv Ital Anat Embryol 1961; 66: 36-62.
  17. Nakane Y, Tsudzuki M. Development of the skeleton in Japanese quail embryos. Dev Growth Differ 1999; 41(5): 523-534.
  18. Hamburger V, Hamilton HL. A series of normal stages in the development of the chick embryo. J Morphol 1951; 88: 49-92.
  19. Padgett CS, Ivey WD. The normal embryology of the coturnix quail. Anat Rec 1960; 37(1): 1-11.
  20. Peters PWJ. Double staining of fetal skeletons for cartilage and bone. In: Neubert D, Merker HJ, Kwasogroh TE (Eds). Methods in prenatal toxicology. Stuttgart, Germany: Georg Thieme 1977; 153-154.
  21. Baumel JJ, Witmer LM. Osteologia. In: Baumel JJ, King AS, Breazile JE, et al (Eds). Handbook of avian anatomy: Nomina anatomica avium. Cambridge, USA: Publications of the Nuttall Ornithological Club 1993; 45-132.
  22. Fujioka T. Time and order of appearance of ossification centers in the chicken skeleton. Acta Anat Nippon 1955; 30: 140-150.
  23. Pourlis A F, Magras IN, Petridis ID. Ossification and growth rates of the limb long bones during the prehatching period in the quail (Coturnix coturnix japonica). Anat Histol Embryol 1998; 27(1): 61-63.
  24. Pourlis AF, Antonopoulos J. The ossification of the pectoral girdle and wing skeleton of the quail (Coturnix coturnix japonica). Anat Histol Embryol 2011;40(3): 219-225.
  25. Pourlis AF, Antonopoulos J. The ossification of the pelvic girdle and leg skeleton of the quail (Coturnix coturnix japonica). Anat Histol Embryol 2014; 43(4): 294-300.
  26. Pourlis AF, Antonopoulos JK, Magras IN. A light and electron microscopic study of the limb long bones perichondral ossification in the quail embryo (Coturnix coturnix japonica). Ital J Anat Embryol 2006; 111(3): 159-170.
  27. Starck JM. Comparative morphology and cytokinetics of skeletal growth in hatchlings of altricial and precocial birds. Zool Anz 1996; 235(1-2): 53-75.
Veterinary Research Forum
Volume 10, Issue 1
Winter 2019
Pages 1-7

  • Receive Date 12 February 2019

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