Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H9N2 in turkey by real time PCR

Document Type: Original Article

Authors

1 DVSc Graduate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

2 Chief of Poultry Diseases Specialized Hospital, Khoy, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

4 Department of Avian Diseases, Razi Vaccine and serum research institute, Karaj, Iran

5 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of 120 day-old commercial male turkeys were purchased and blood samples were collected from 20 day-old turkeys, remaining 100 were divided into four equal groups and kept in separated rooms. Groups 1 and 2 were infected with 104 CID50 of IBDV via intra-bursal route on day 1; Groups 1 and 3 were each infected with 106 EID50of AIV (H9N2) via the oculo-nasal routes on day 30. All groups were vaccinated against Newcastle disease vaccine (NDV). Detection of avian influenza virus H9N2 in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction. Anti- NDV–AIV and anti-IBD titers were measured using HI and ELISA tests, respectively. The present study showed that infectious bursal disease changed the pathogenesis of (AIV) H9N2 by affecting AI virus replication and resulted in an increase shedding due to prolonged duration of sever clinical signs. The extent of shedding and virus replication need further study.

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  1. Capua L, Alexander DJ. Avian influenza: Recent developments. Avian Pathol 2004; 33(4): 393-404.
  2. Knipe DM, Howely PM. Fields virology. 5th ed. Philadelphia, USA: Williams & Wilkins 2007;
    1647-1690.
  3. Tong S, Li Y, Rivailler P, et al. A distinct lineage of influenza A virus from bats. Proc Natl Acad Sci USA 2012; 109(11): 4269-4274.
  4. Nili H, Asasi K. Natural cases and an experimental study of H9N2 avian influenza in commercial broiler chickens of Iran. Avian Pathol 2002; 31(3): 247-252.
  5. Vasfi Marandi M, Bozorgmehri Fard MH. Isolation of H9N2 subtype of Avian Influenza Viruses during an outbreak in chickens in Iran. Iran Biomed J 2002; 6 (1): 13-17.
  6. Swieton E, Jozwiak M, Minta Z, et al. Genetic characterization of H9N2 avian influenza viruses isolated from poultry in Poland during 2013/2014. Virus Genes 2018; 54(1): 67-76.
  7. Smietanka K, Minta Z, Swieton E, et al. Avian influenza H9N2 subtype in Poland – characterization of the isolates and evidence of concomitant infections. Avian Pathol 2014; 43(5): 427-436.
  8. Capua I, Mutinelli F, Marangon S, et al. H7N1 avian influenza in Italy (1999 to 2000) in intensively reared chickens and turkeys. Avian Pathol2000; 29(6): 537-543.
  9. Halvorson DA, Kelleher CJ, Senne DA. Epizootiology of avian influenza: Effect of season on incidence in sentinel ducks and domestic turkeys in Minnesota. Appl Environ Microbiol 1985; 49(4): 914-919.
  10. Halvorson DA. Twenty-five years of avian influenza in Minnesota. In Proceedings: The 53rd North Central Avian Disease Conference. Minneapolis, USA 2002; 65-69.
  11. Swayne DE, Suarez DL, Sims LD. Influenza. In: Swayne DE, Glisson JR, Mc Dougald LR, et al. (Eds). Disease of poultry. 13th ed. Ames, USA: Wiley-Blackwell Inc. 2013; 181-218.
  12. Eterradossi N, Saif YM. Infectious bursal disease In: Swayne DE, Glisson JR, McDougald LR, et al. Disease of poultry. 13th ed. Ames, USA: Wiley-Blackwell Inc. 2013; 433-246.
  13. Razmyar J, Peighambari SM. Isolation and characterization of a very virulent Infectious bursal disease virus from turkey. Acta Virol 2009; 53(4): 271-276.
  14. Owoade AA, Mulders MN, Kohnen J, et al. High sequence diversity in infectious bursal disease virus serotype 1 in poultry and turkey suggests West-African origin of very virulent strains. Arch Virol 2004; 149(4): 653-672.
  15. Jackwood DJ, Stoute ST, Crossley BM. Pathogenicity of genome reassortant infectious bursal disease viruses in chickens and Turkeys. Avian Dis 2016; 60(4): 765-772.
  16. Reed LJ, Muench H. A simple method of estimating fifty percent endpoint. Am J Hyg 1938; 27(3): 493-497.
  17. Mayahi M, Bouzorghmehri Fard MH. The effect of infectious bursal disease virus and cyclophosphamide on immune response against Newcastle disease vaccination in broiler chicks. Indian J Anim Sci 2000; 70(1): 8-10.
  18. Azizpour A, Goudarzi H, Charkhkar S, et al. Experimental study on tissue tropism and dissemination of H9N2 avian influenza virus and ornithobacterium rhinotracheale coinfection in SPF chickens. J Anim Plant Sci 2014; 24(6): 1655-1662.
  19. Lee CW, Suarez DL. Application of real-time RT-PCR for the quantitation and competitive replication study of H5 and H7 subtype avian influenza virus. J Virol Methods 2004; 119(2):151-158.
  20. Dohms JE, Lee KP, Rosenberger JK. Plasma cell changes in the gland of Harder infectious bursal disease virus infection of the chicken. Avian Dis 1981; 25(3): 683-695.
  21. Edwards K, Logan J, Saunders N. Real-time PCR: An essential guide. Norfolk, UK: Horizon Bioscience 2004; 346.
  22. Swayne DE, Slemons RD. Comparative pathology of a chicken origin and two duck origin influenza virus isolated in chickens, the effect of rout of inoculation. Vet Pathol. 1994; 31(2): 237-245.
  23. Mosleh N, Dadras H, Mohammadi A. Molecular quantitation of H9N2 avian influenza virus in various organs of broiler chickens using TaqMan real time PCR. J Mol Genet Med. 2009; 3(1): 152-157.
  24. Swayne DE, Halvorson DA. Influenza. In: Saif YM, Barnes HJ (Eds). Disease of poultry. 11th ed. Iowa, USA: University Press 2003; 135-160.
  25. Chui C, Thorsen J. Experimental infection of turkeys with infectious bursal disease virus and the effect on the immunocompetence of infected turkeys. Avian Dis 1984; 28(1):197-207.
  26. Faragher JT, Allan WH, Wyeth CJ. Immuno­suppressive effect of infectious bursal agent on vaccination against Newcastle disease. Vet Rec 1974; 95(17):385-388.
  27. Kwon JS, Lee HJ, Lee DH, et al. Immune responses and pathogenesis in immunocompromised chickens in response to infection with H9N2 low pathogenic avian influenza virus. Virus Res 2008; 133(2):187-194.
  28. Swayne DE, Slemons RD. Comparative pathology of intravenously inoculated wild duck and turkey origin type A influenza virus in chickens. Avian Dis 1995; 39(1):74-84.
  29. Swayne DE, Slemons RD. Renal pathology in specific-pathogen-free chickens with a water fowl-origin type A influenza virus. Avian Dis 1990; 34(2): 285-297.
  30. Shalaby AA, Slemons RD, Swayne DE. Pathological studies of A/Chicken/Alabama/7395/75(H4N8) influenza virus in specific pathogen free laying hens. Avian Dis 1994; 38(1): 22-32.
  31. Swayne DE, Slemons RD. Evaluation of the kidney as a potential site of avian influenza virus persistence in chickens. Avian Dis. 1992; 36(4): 937-944.
  32. Hablolvarid MH, Sohraby-Haghdost I, Pourbakhsh SA, et al. A study on histopathologic changes in chicken following intravenous inoculation with avian influenza virus A/Chicken/Iran/259/1998 (H9N2) Arch Razi Ins 2003; 55: 41-54.