Veterinary Research Forum
Vet Res Forum

Experimental study on healing of long bone defects treated with fibrin membrane enriched with platelet growth factors and periosteal mesenchymal stem cells in rabbit: radiographical and histopathological evaluations

Document Type : Original Article

Authors

Amin Paidar Ardakani 1
Mohammd Mehdi Oloumi 2
Alireza Farsinejad 3
Reza Kheirandish 4

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ardakan University, Ardakan, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Iran

3 Department of Hematology and Medical laboratory Sciences, Faculty of Allied Medicine, Kerman University of Medical Sciences, Kerman, Iran

4 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

https://doi.org/10.30466/vrf.2018.86692.2124
Abstract
The present study was designed to evaluate the effects of platelet growth factors and periosteal mesenchymal stem cells on bone healing process, radiographically. Forty male White New Zealand rabbits in five equal groups were used in this study. A 2 mm full thickness bone defect was made in left radial bone of each animal. In group A (control) the defect was left with no medical intervention. In group B the defect was covered by a fibrin membrane. In group C the defect was covered by a fibrin membrane plus platelet growth factors. In group D the defect was covered by a fibrin membrane plus periosteal mesenchymalstem cells, and in group E the defect was covered by a fibrin membrane enriched with platelet growth factors and periosteal mesenchymalstem cells. Radiological evaluation was done immediately after surgery (week 0) and then at the 1st, 2nd, 4th, 6th and 8th weeks after operation. At the end of the eighth week, bone samples were taken to evaluate the histopathology. The radiological and histopathological observations showed a superior bone healing in the groups D and E, after eight weeks in comparison with the groups A, B and C. According to this study, it could be concluded that the platelet growth factors and periosteal mesenchymalstem cells could promote bone regeneration in long bone defects in a rabbit model.

Keywords

Bone healing
Fibrin membrane
Platelet growth factor
Rabbit
Stem cell

Subjects

  1.  

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Veterinary Research Forum
Volume 10, Issue 4
Autumn 2019
Pages 285-291

  • Receive Date 24 May 2018
  • Revise Date 12 September 2018
  • Accept Date 18 September 2018

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