Histopathological evaluation of the role of negative electrical charge on renal ischemia/reperfusion injuries on brain and heart tissues in rat

Document Type: Original Article

Authors

1 DVM Graduate, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

10.30466/vrf.2018.86965.2126

Abstract

This study was performed to evaluate the role of electroacupuncture on kidney 1 (Ki1) acupoint to prevent the heart and brain injury following ischemia/reperfusion of both kidneys. 24 Sprague Dawley rats were randomly assigned into four equal groups. In the treatment 1 group, following anesthesia, acupuncture needles were inserted on Ki1 on the palm of both hindlimbs and connected to electroacupuncture unit for a 3.00 Hz direct current, 1 hr before surgery until the end of surgery. In treatment two groups, the electroacupuncture was also performed 48 and 24 hr before the operation, with the same protocol as treatment 1. Control 1 and control 2 groups had the same procedures like the treatment ones, except for acupuncture. Immediately after reperfusion, the samples of brains and hearts were taken and prepared for microscopic examination. Histopathological study of the heart in the control and treatment groups showed the breakage of myofibrils, hyaline necrosis, edema and disorganization of myocytes. The severity of cardiac lesions was decreased in both treatment groups in comparison with the controls. Brain in control and treatment groups showed ischemic necrosis, disorganization of the neurons in the hippocampus, and edema. The severity of lesions was reduced in the treatment groups and showed a significant difference between the control and treatment 1. It could be concluded that electroacupuncture on the Ki1 point could reduce the severity of damages induced by renal ischemia/reperfusion in the remote organs of the heart and brain.

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  1. Vishwakarma VK, Upadhyay PK, Gupta JK, et al. Pathophysiologic role of ischemia reperfusion injury: A review. J Indian Coll Cardiol 2017; 7(3): 97-104.
  2. Matern C. Acupuncture for dogs and cats: A pocket atlas. 1st ed. New York, USA: Thieme 2011; p2.
  3. Zhao JX, Tian YX, Xiao HL, et al. Effects of electro-acupuncture on hippocampal and cortical apoptosis in a mouse model of cerebral ischemia-reperfusion injury. J Tradit Chin Med 2011; 31(4): 349-355.
  4. Zhao JX, Xu H, Tian YX, et al. Effect of electro-acupuncture on brain-derived neurotrophic factor mRNA expression in mouse hippocampus following cerebral ischemia-reperfusion injury. J Tradit Chin Med 2013; 33(2): 253-257.
  5. Yu L, Liao Y, Wu H, et al. Effects of electroacupuncture and Chinese kidney-nourishing medicine on polycystic ovary syndrome in obese patients. JTradit Chin Med 2013; 33(3): 287-293.
  6. Yili W, Yuanxiang T, Jianxin Z, et al. Effect of electroacupuncture on gene expression in calcium signaling pathway in hippocampal cells in mice with cerebral ischemia reperfusion. J Tradit Chin Med 2017; 37(2): 252-260.
  7. Radovsky A, Katz L, Ebmeyer U, et al. Ischemic neurons in rat brains after 6, 8, or 10 minutes of transient hypoxic ischemia. Toxicol Pathol 1997; 25(5):500-505.
  8. Frias Neto CA, Koike MK, Saad KR, et al. Effects of ischemic preconditioning and cilostazol on muscle ischemia-reperfusion injury in rats. Acta Cir Bras2014; 29(Suppl 3): 17-21.
  9. Bulkley GB. Free radical-mediated reperfusion injury: A selective review. Br J Cancer Suppl 1987; 8: 66-73.
  10. Baumgartner WA, Williams GM, Fraser CD Jr, et al. Cardiopulmonary bypass with profound hypothermia. An optimal preservation method for multiorgan procurement. Transplantation 1989; 47(1): 123-127.
  11. Vishwakarma VK, Qureshi SS, Agrawal V, et al. Role of atrial natriuretic peptides in various conditions. Int J Pharm Biol Sci 2016; 7(3): 20-27.
  12. Adibhatla RM, Hatcher JF. Lipid oxidation and peroxidation in CNS health and disease: From molecular mechanisms to therapeutic opportunities. Antioxid Redox Signal 2010; 12(1): 125-169.
  13. Han J, Wang D, Yu B, et al. Cardioprotection against ischemia-reperfusion by licochalcone B in isolated rat hearts. Oxid Med Cell Longev 2014; 2014: 134862. doi:10.1155/2014/134862.
  14. Zhang S, Li H, Yang SJ. Tribulosin protects rat hearts from ischemia/reperfusion injury. Acta Pharmacol Sin 2010; 31(6): 671-678.
  15. Maxwell SR, Lip GY. Reperfusion injury: A review of the pathophysiology, clinical manifestations and therapeutic options. Int J Cardiol 1997; 58(2): 95-117.
  16. Park JL, Lucchesi BR. Mechanisms of myocardial reperfusion injury. Ann Thorac Surg 1998; 68(5): 1905-1912.
  17. Chandrashekhar VM, Ranpariya VL, Ganapaty S, et al. Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats. J Ethnopharmacol 2010; 127(3): 645-651.
  18. Garcia JH, Kalimo H, Kamijyo Y, et al. Cellular events during partial cerebral ischemia. I. Electron microscopy of feline cerebral cortex after middle-cerebral-artery occlusion. Virchows Arch B cell Pathol 1977; 25(3): 191-206.
  19. Petito CK, Babiak T. Early proliferative changes in astrocytes in postischemic noninfarcted rat brain. Ann Neurol 1982; 11(5): 510-518.
  20. Lukaszevicz AC, Sampaïo N, Guégan C, et al. High sensitivity of protoplasmic cortical astroglia to focal ischemia. J Cereb Blood Flow Metab 2002; 22(3): 289-298.
  21. Pantino L, Garcia JH, Gutierrez JA. Cerebral white matter is highly vulnerable to ischemia. Stroke 1996; 27(9): 1641-1647.
  22. Inchauspe AA. Traditional Chinese medical criteria about the use of Yongquan as a life support maneuver. In: Kuang H (Ed). Recent advances in theories and practice of Chinese medicine. Shanghai, China: InTech 2012; 361-368.
  23. Yu YP, Ju WP, Li ZG, et al. Acupuncture inhibits oxidative stress and rotational behavior in 6-hydroxydopamine lesioned rat. Brain Res 2010; 1336: 58-65.
  24. Zhang X, Wu B, Nie K, et al. Effects of acupuncture on declined cerebral blood flow, impaired mitochondrial respiratory function and oxidative stress in multi-infarct dementia rats. Neurochem Int 2014; 65: 23-29.
  25. Qi YC, Xiao XJ, Duan RS, et al. Effect of acupuncture on inflammatory cytokines expression of spastic cerebral palsy rats. Asian Pac J Trop Med 2014; 7(6): 492-495.
  26. Abe K, Hayashi N, Terada H. Effect of endogenous nitric oxide on energy metabolism of rat heart mitochondria during ischemia and reperfusion. Free Radic Biol Med 1999; 26(3-4): 379-387.
  27. Akhlaghi M, Bandy B. Mechanisms of flavonoid protection against myocardial ischemia-reperfusion injury. J Mol Cell Cardiol 2009; 46(3): 309-317.
  28. Özbal S, Erbil G, Koçdor H, et al. The effects of selenium against cerebral ischemia-reperfusion injury in rats. Neurosci Lett 2008; 438(3): 265-269.
  29. Yamauchi K, Nakano Y, Imai T, et al. A novel nuclear factor erythroid 2-related factor 2 (Nrf2) activator RS9 attenuates brain injury after ischemia-reperfusion in mice. Neuroscience 2016; 333: 302-310.