Veterinary Research Forum
Vet Res Forum

Protective effect of vitamin E on sperm quality and in vitro fertilizing potential and testosterone concentration in polyvinyl chloride treated Male rats

Document Type : Original Article

Authors

Abbas Sadeghi 1
Farah Farokhi 1
Ali Shalizar Jalali 2
Gholamreza Najafi 2

1 Department of Biology, Faculty of Science, Urmia University, Urmia, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2019.91184.2206
Abstract
Polyvinyl chloride (PVC) has toxic effects through the induction of oxidative stress in the body and testicles. Vitamin E (Vit E) is a dietary compound that functions as an antioxidant scavenging toxic free radicals. The present study aimed to probe the protective effect of Vit E against PVC-induced reprotoxicity in male rats. In this experimental study, 24 male rats were randomly divided into four groups (n=6) including control, Vit E (150 mg kg-1 per day; orally), PVC (1000 mg kg-1 per day; orally) and PVC + Vit E. After 40 days, rats were euthanized and epididymal sperms characteristics, embryo development and malondialdehyde (MDA) and testosterone levels were examined. The PVC decreased sperm count, motility and viability as well as testosterone level and increased sperms with damaged chromatin in comparison with controls. Also, the percentages of fertilization, two-cell embryos and blastocysts as well as MDA levels were decreased in PVC-treated rats. However, Vit E improved PVC-induced alterations in aforesaid parameters. The results indicated that PVC can reduce fertility potential in male rats probably through androgen and sperm quality and quantity reductions, while Vit E can exert protective effects in PVC-related reproductive toxicities.

Keywords

Embryology
Polyvinyl Chloride
Rat
Spermatology
Vitamin E

  1.  

    1. LaDou J. Current occupational and environmental medicine. 3rd ed. California, USA: McGraw Hill Professional 2003; 258.
    2. Keys DA, Wallace DG, Kepler TB, et al. Quantitative evaluation of alternative mechanisms of blood and testes disposition of di (2-ethylhexyl) phthalate and mono (2-ethylhexyl) phthalate in rats. Toxicol Sci 1999; 49(2): 172-185.
    3. Zare Z, Eimani H, Mohammadi MO, et al. Histo-pathological study of di-(2-ethylhexyl) phthalate (DEHP) on testes in mouse. J Mazandaran Univ Med Sci 2009; 19(71): 52-59.
    4. Latini G, Ferri M, Chiellini F. Material’s degradation in PVC medical devices, DEHP leaching and neonatal outcomes. Curr Med Chem 2010; 17(26): 2979-2989.
    5. Ishihara M, Itoh M, Miyamoto K, et al. Spermatogenic disturbance induced by di-(2-ethylhexyl) phthalate is significantly prevented by treatment with antioxidant vitamins in the rat. Int J Androl 2000; 23(2): 85-94.
    6. Dirven HA, van den Broek PH, Arends AM, et al. Metabolites of the plasticizer di (2-ethylhexyl) phthalate in urine samples of workers in polyvinylchloride processing industries. Int Arch Occup Environ Health 1993; 64(8): 549-554.
    7. Wagoner JK. Toxicity of vinyl chloride and poly (vinyl chloride): A critical review. Environ Health Perspect 1983; 52: 61-66.
    8. Parmar D, Srivastava SP, Singh GB, et al. Effect of testosterone on the testicular atrophy caused by di (2-ethylhexyl) phthalate (DEHP). Toxicol Lett 1987; 36(3): 297-308.
    9. Mathur PP, Dcruz SC. The effect of environmental contaminants on testicular function. Asian J Androl 2011; 13(4): 585-591.
    10. Kasahara E, Sato EF, Miyoshi M, et al. Role of oxidative stress in germ cell apoptosis induced by di (2-ethyl-hexyl) phthalate. Biochem J 2002; 365(3): 849-856.
    11. Fraga CG, Motchnik PA, Shigenaga MK, et al. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc Natl Acad Sci USA 1991; 88(24): 11003-11006.
    12. Kodama H, Yamaguchi R, Fukuda J, et al. Increased oxidative deoxyribonucleic acid damage in the spermatozoa of infertile male patients. Fertil Steril 1997; 68(3): 519-524.
    13. Aitken RJ. Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol Reprod Dev 2017; 84(10): 1039-1052.
    14. Lobascio AM, De Felici M, Anibaldi M, et al. Involve-ment of seminal leukocytes, reactive oxygen species, and sperm mitochondrial membrane potential in the DNA damage of the human spermatozoa. Andrology 2015; 3(2): 265-270.
    15. El-Demerdash FM. Antioxidant effect of vitamin E and selenium on lipid peroxidation, enzyme activities and biochemical parameters in rats exposed to aluminium. J Trace Elem Med Biol 2004; 18(1): 113-121.
    16. Sadeghi A, Farokhi F, Najafi G, et al. Protective effect of vitamin E against polyvinyl chloride induced damages and oxidative stress in rat testicular tissue. J Kermanshah Univ Med Sci 2017; 21(3): 96-102.
    17. Selvakumar E, Prahalathan C, Sudharsan PT, et al. Chemoprotective effect of lipoic acid against cyclophosphamide-induced changes in the rat sperm. Toxicology 2006; 217(1): 71-78.
    18. Zambrano E, Rodriguez-Gonzalez GL, Guzman C, et al. A maternal low protein diet during pregnancy and lactation in the rat impairs male reproductive development. J Physiol 2005; 563(1): 275-284.
    19. Erenpreiss J, Bars J, Lipatnikova V, et al. Comparative study of cytochemical tests for sperm chromatin integrity. J Androl 2001; 22(1): 45-53.
    20. Wyrobek AJ, Gordon LA, Burkhart JG, et al. An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. A report of the US environmental protection agency gene-tox program. Mutat Res 1983; 115(1): 1-72.
    21. Menkveld R, Wong WY, Lombard CJ, et al. Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: An effort towards standardization of in-vivo thresholds. Hum Reprod 2001; 16(6): 1165–1171.
    22. Suckow MA, Weisbroth SH, Franklin CL. The laboratory rat. 2nd ed. New York, USA: Elsevier Academic Press 2006; 165-173.
    23. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Methods Enzymol 1990; 186: 407-421.
    24. Huang LP, Lee CC, Fan JP, et al. Urinary metabolites of di (2-ethylhexyl) phthalate relation to sperm motility, reactive oxygen species generation, and apoptosis in polyvinyl chloride workers. Int Arch Occup Environ Health 2014; 87(6): 635-646.
    25. Esteves SC. Clinical relevance of routine semen analysis and controversies surrounding the 2010 World Health Organization criteria for semen examination. Int Braz J Urol 2014; 40(4): 433-453.
    26. Cohen-Bacrie P, Belloc S, Menezo YJ, et al. Correlation between DNA damage and sperm parameters: A prospective study of 1,633 patients. Fertil Steril 2009; 91(5): 1801-1805.
    27. Mathur PP, Huang L, Kashou A, et al. Environmental toxicants and testicular apoptosis. Open Reprod Sci J 2011; 3(1): 114-124.
    28. Palleschi S, Rossi B, Diana L, et al. Di (2-ethylhexyl) phthalate stimulates Ca2+ entry, chemotaxis and ROS production in human granulocytes. Toxicol Lett 2009; 187(1): 52-57.
    29. Erkekoglu P, Rachidi W, Yuzugullu OG, et al. Evaluation of cytotoxicity and oxidative DNA damaging effects of di (2-ethylhexyl)-phthalate (DEHP) and mono (2-ethylhexyl)-phthalate (MEHP) on MA-10 Leydig cells and protection by selenium. Toxicol Appl Pharmacol 2010; 248(1): 52-62.
    30. Aitken RJ, De Iuliis GN, Finnie JM, et al. Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria. Hum Reprod 2010; 25(10): 2415-2426.
    31. Hughes CM, Lewis SE, McKelvey-Martin VJ, et al. The effects of antioxidant supplementation during Percoll preparation on human sperm DNA integrity. Hum Reprod 1998; 13(5): 1240-1247.
    32. Catt JW, Henman M. Toxic effects of oxygen on human embryo development. Hum Reprod 2000; 15(2):199-206.
    33. Wang X, Falcone T, Attaran M, et al. Vitamin C and vitamin E supplementation reduce oxidative stress–induced embryo toxicity and improve the blastocyst development rate. Fertil Steril 2002; 78(6): 1272-1277.
    34. Ouchi A, Nagaoka SI, Abe K, et al. Kinetic study of the aroxyl radical-scavenging reaction of α-tocopherol in methanol solution: Notable effect of the alkali and alkaline earth metal salts on the reaction rates. J Phys Chem 2009; 113(40): 13322-13331.
    35. Sharpe RM, Donachie K, Cooper I. Re-evaluation of the intratesticular level of testosterone required for quantitative maintenance of spermatogenesis in the rat. J Endocrinol 1988; 117(1): 19-26.
    36. Pan G, Hanaoka T, Yoshimura M, et al. Decreased serum free testosterone in workers exposed to high levels of di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP): a cross-sectional study in China. Environ Health Perspect 2006; 114(11): 1643-1648.
    37. Odonnell L, McLachlan RI, Wreford NG, et al. Testosterone promotes the conversion of round spermatids between stages VII and VIII of the rat spermatogenic cycle. Endocrinology 1994; 135(6): 2608-2614.
    38. Sun YT, Wreford NG, Robertson DM, et al. Quantitative cytological studies of spermatogenesis in intact and hypophysectomized rats: Identification of androgen-dependent stages. Endocrinology 1990; 127(3): 1215-1223.
    39. Jalali AS, Najafi G, Hosseinchi M, et al. Royal jelly alleviates sperm toxicity and improves in vitro fertilization outcome in stanozolol-treated mice. Iran J Reprod Med 2015; 13(1): 15-22.
    40. Gil-Guzman E, Ollero M, Lopez MC, et al. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum Reprod 2001; 16(9): 1922-1930.
    41. Hassa H, Gurer F, Tanir HM, et al. Effect of cigarette smoke and alpha-tocopherol (vitamin E) on fertilization, cleavage, and embryo development rates in mice: An experimental in vitro fertilization mice model study. Eur J Obstet Gynecol Reprod Biol 2007; 135(2): 177-182.
Veterinary Research Forum
Volume 11, Issue 3
Summer 2020
Pages 257-263

  • Receive Date 02 August 2018
  • Revise Date 06 February 2019
  • Accept Date 05 March 2019

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