Veterinary Research Forum
Vet Res Forum

Designing an enzyme-linked immunosorbent assay for detection of anti-penicillin antibodies levels in various species of animals in Khuzestan province, Iran

Document Type : Short Communication

Authors

Mohammad Khosravi 1
Mohammad Rahim Haji Hajikolaie 2
Shahrzad Alipour 3
Ara Ameri 3
Mohammad Bafandeh Dehaghi 3

1 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 DVM Graduate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.30466/vrf.2019.95242.2293
Abstract
The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the reactive antibody value against penicillin in various species of animals. The serum samples were collected from nine species (forty mature animals in each species) including horse, dog, goat, sheep, buffalo, cattle, donkey, chicken, and fish. The concentrations of total antibody and immunoglobulin M (IgM) against penicillin were detected using an in-house ELISA test. The total anti-penicillin antibodies concentration from high to low in animals was as chicken, horse, fish, donkey, dog, goat, sheep, buffalo, and cattle, respectively. In cattle and sheepthe level of anti-penicillin IgM (APM) was significantly higher than non-IgM antibodies (APNM); moreover, levels of APNM were very low in chicken and fish serums; no difference was seen regarding these values in buffalo and goat. The other species had significantly lower APM than the APNM. The ani-penicillin antibody levels in the noted animals were successfully detected using the developed ELISA. Most of the species have anti-penicillin antibodies; however, they have reactive antibodies with differences in levels and isotypes.

Keywords

Animals
Antibody
Enzyme-linked immunosorbent assay
Penicillin

  1.  

    1. Thway TM, Magana I, Bautista A, et al. Impact of anti-drug antibodies in preclinical pharmacokinetic assessment. AAPS J 2013; 15(3): 856-863.
    2. Chirmule N, Jawa V, Meibohm B. Immunogenicity to therapeutic proteins: Impact on PK/PD and efficacy. AAPS J 2012; 14 (2): 296-302.
    3. Garratty G. Drug-induced immune hemolytic anemia. Hematology Am Soc Hematol Educ Program 2009; 1: 73-79.
    4. Partridge MA, Purushothama SH, Elango C, et al. Emerging technologies and generic assays for the detection of anti-drug antibodies. J Immunol Res 2016; 2016: 6262383. doi:10.1155/2016/6262383.
    5. Gomez MB, Torres MJ, Mayorga C, et al. Immediate allergic reactions to betalactams: Facts and controversies. Curr Opin Allergy Clin Immunol 2004; 4(4): 261-266.
    6. Celik W, Pochler WJ, Adkinson NF. Drug allergy. In: Adkinson Jr N, Bochner B, Burks A, et al. (Eds). Middleton’s allergy: Principles and practice. Philadelphia, USA: Elsevier Saunders 2009; 1205-1226.
    7. Mirakian R, Leech SC, Krishna MT, et al. Management of allergy to penicillins and other beta-lactams. Clin Exp Allergy 2015; 45(2): 300-327.
    8. Mera K, Nagai M, Brock JW, et al. Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products. J Immunol Methods 2008; 334(1-2): 82-90.
    9. Hay FC, Westwood OMR. Practical immunology. 4th ed. New York, USA: John Wiley & Sons 2002; 23; 114-115.
    10. Khoobdel M, Nayeri Fasaei B, Zahraei Salehi T, et al. The production of anti-idiotype antivenom against Mesobuthus eupeus (Scorpionida: Buthidae) venom in rabbits. Toxicon 2013; 76: 44-49.
    11. Nishikawa AH, Bailon P. Affinity purification methods. Improved procedures for cyanogen bromide reaction on agarose. Anal Biochem 1975; 64: 268-275.
    12. Wang SJ, Wu ST, Gokemeijer J, et al. Attribution of the discrepancy between ELISA and LC-MS/MS assay results of a PEGylated scaffold protein in post-dose monkey plasma samples due to the presence of anti-drug antibodies. Anal Bioanal Chem 2012; 402(3), 1229-1239.
    13. Song S, Yang L, Trepicchio WL, et al. Understanding the supersensitive anti-drug antibody assay: Unexpected high anti-drug antibody incidence and its clinical relevance. J Immunol Res 2016; 2016: 3072586.
    14. Lee D, Dewdney JM, Edwards RG, et al. Measurement of specific IgG antibody levels in serum of patients on regimes comprising high total dose beta-lactam therapy. Int Arch Allergy Appl Immunol 1986; 79(4): 344-348.
    15. Meulenbroek LA, de Jong RJ, den HartogJager CF, et al. IgG antibodies in food allergy influence allergen-antibody complex formation and binding to B cells: A role for complement receptors. J Immunol 2013; 191(7): 3526-3533.
    16. Qiao HL, Gao N, Jia LJ, et al. Specific IgG antibodies in sera in patients with penicillin allergy. Clin Exp Med 2009: 9 (2): 105-111.
    17. Christie G, Coleman JW, Newby S, et al. A survey of the prevalence of penicillin-specific IgG, IgM and IgE antibodies detected by ELISA and defined by hapten inhibition, in patients with suspected penicillin allergy and in healthy volunteers. Br J Clin Pharmacol 1998: 25(3): 381-386.
    18. Maraschiello C. The relevance of immunogenicity in preclinical development. J Bioanal Biomed 2014; 6(1): 001-004.
    19. Krishna M, Nadler SG. Immunogenicity to bio-therapeutics – The role of anti-drug immune complexes. Front Immunol 2016; 7: 21.
    20. Schafer M, Challand S, Schick E, et al. An ex vivo potency assay to assess active drug levels of a GLP-1 agonistic peptide during preclinical safety studies. Bioanalysis 2015; 7(24): 3063-3072.
Veterinary Research Forum
Volume 11, Issue 3
Summer 2020
Pages 285-288

  • Receive Date 08 October 2018
  • Revise Date 26 December 2018
  • Accept Date 19 January 2019

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