Veterinary Research Forum
Vet Res Forum

Metabolomic profiling of liver tissues after acute administration of vardenafil in rats

Document Type : Original Article

Authors

Onur Şenol 1
Şaziye Sezin Palabiyik 2
Beyzagül Polat 3
Yücel Kadioğlu 4
Mehmet Emrah Yaman 1

1 Department of Analytical Chemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey

2 Department of Toxicology, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey

3 Department of Pharmacology, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey

4 Department of Analytical Chemistry, Faculty of Pharmacy, Atatürk University, Erzurum, Turkey

https://doi.org/10.30466/vrf.2019.95959.2302
Abstract
Erectile dysfunction (ED) diseases have almost affected 100 million men all over the world. Orally administered phosphodiesterase 5 (PDE 5) inhibitors are the most used pharmaceutical formulations for the treatment of ED. In this study, it is aimed to investigate the metabolomics feature of orally administered vardenafil in rats. To carry out the experimental procedure eight male Wistar albino rats were used. Their livers were gently removed and metabolomics profiles of each sample were determined by UPLC Q-TOF MS. Identification of metabolites was achieved by the METLIN database. Cluster analysis was also performed via Principle Component Analysis. Several metabolites were identified and results were evaluated by XCMS software. UPLC Q-TOF MS could be successfully applied to profile biomarkers and help us understand the molecular mechanisms of vardenafil usage. It was concluded that the level of some metabolites, responsible for the collagen synthesis and Kreb’s cycle, has been statistically significant after the vardenafil administration.

Keywords

Erectile dysfunction
Kreb’s cycle
Metabolomics
Q-TOF MS
Vardenafil

  1.  

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Veterinary Research Forum
Volume 11, Issue 3
Summer 2020
Pages 213-217

  • Receive Date 18 October 2018
  • Revise Date 05 December 2018
  • Accept Date 19 January 2019

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