Veterinary Research Forum
Vet Res Forum

Histopathological and cytological analyses of endometrium in water ‎buffaloes (Bubalus bubalis) to detect estrus and endometritis

Document Type : Original Article

Authors

Zahra Salemi 1
Annahita Rezaie 2
Saad Goorani Nejad 3
Babak Mohammadian 2

1 DVM Graduate, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.30466/vrf.2019.92037.2226
Abstract
This study aimed to determine cytological, histopathological and cytomorphometrical characteristics of endometrium in healthy and endometritic uterus in the water buffalo. Fifty eight non-pregnant reproductive systems were collected from slaughterhouse. Efficiency of three methods of sampling including cotton swab, smear, and aspiration were compared for cytologic study. Concurrent histopathologic examination revealed endometritis in 38 uteri including 8 (21.00%) with mild endometritis, 7 (18.42%) with moderate endometritis, 6 (15.90%) with severe endometritis and 17 (44.73%) with chronic endometritis. Cytomorphometrical results showed significant relationship between diameter and area of epithelial nuclei with phases of estrus cycle. Neutrophil and lymphocytes densities in swab and aspiration samples were significantly higher in severe endometritis than normal and chronic endometritis samples. Similarly, lymphocytes density in smear and aspiration methods was significant between normal and moderates, and also severe and chronic endometritis. Cytomorphometric analysis of epithelial nuclei characteristics (diameter and area) in buffalo were performed for the first time and it could be valuable to identify estrus cycle in this species. Aspiration had the most efficiency to identify endometritis in comparison with other methods.

Keywords

Bubalus bubalis
‎ Cytology
‎ Cytomorphometry
‎ Endometritis
‎ Histopathology
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Veterinary Research Forum
Volume 11, Issue 4
Autumn 2020
Pages 409-414

  • Receive Date 16 August 2018
  • Revise Date 08 February 2019
  • Accept Date 09 March 2019

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