Histopathological, cytological analysis of endometrium in Water buffaloes (Bubalus bubalis) to detect estrus and endometritis

Document Type: Original Article



The aim of this study was to determine cytological, histopathological and cytomorphometrical characteristics of endometrium in healthy and endometritic uterus in Water buffalo. Fifty eight non-pregnant reproductive systems were collected from slaughterhouse. Efficiency of three methods of sampling including cotton swab, smear, and aspiration were compared for cytologic study. Concurrent histopathologic examination revealed endometritis in 38 uteri including 8 uteri (21%) with mild endometritis, 7 uteri (18/42%) with moderate endometritis, 6 uteri (15/9%) with severe endometritis and 17 uteri (44/73%) with chronic endometritis. Cytomorphometrical results showed significant relationship between diameter and area of epithelial nuclei with phases of estrus cycle. Neutrophil and lymphocytes densities in swab and aspiration samples were significantly higher in severe endometritis than normal and chronic endometritis samples. Similarly, lymphocytes density in smear and aspiration methods was significant between normal and moderates, and also severe and chronic endometritis. Cytomorphometric analysis of epithelial nuclei characteristics (diameter and area) in buffalo were performed for the first time and it can be valuable to identify estrus cycle in this species. Aspiration had the most efficiency to identify endometritis in comparison with other methods.


Articles in Press, Accepted Manuscript
Available Online from 29 September 2020
  • Receive Date: 16 August 2018
  • Revise Date: 08 February 2019
  • Accept Date: 09 March 2019