Use of Ovine Acellular Peritoneal Matrix combined with Honey and Ovine Fetal Skin Extract in healing of full-thickness infected burn wounds in a rat model

Document Type: Original Article


1 Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran



Treatment of infected burn wounds still remains a challenge in burn units. Silver-sulfadiazine (SSD) is the most commonly used topical antimicrobial agent in managing these wounds. We aimed to accelerate healing of burn wounds by combined application of ovine acellular peritoneal matrix (OAPM), honey (H) and ovine fetal skin extract (OFSE). Sixty-four standardized burn wounds were created on dorsum of 16 rats and were subsequently inoculated with Staphyloccocus aureus and Pseudomonas aeruginosa. After 48 hours, the wounds were surgically debrided and received either physiologic saline (control group) or SSD, OAPM+ SSD, OAPM+ H+ SSD, OAPM+ H+ OFSE+ SSD. The healing wounds were evaluated for size, bacterial counts, histopathology and biomechanical properties on days 3, 7, 14, 21 and 28 after surgery. All treatments had effectively reduced the level of S. aureus and P. aeruginosa on wounds compared to the control group by day 3 and 7. The wounds treated with combined application of OAPM+ H+ OFSE+ SSD demonstrated considerable inflammation reduction, fibroplasia, complete re-epithelialization and wound contraction together with significantly lesser scar tissue formation. Treatment with OAPM+ H+ OFSE+ SSD has shown superior biomechanical properties of the healing wounds. The findings suggest that the synergistic effect of dressing the wounds with OAPM, H and OFSE was a very effective approach in accelerating the healing process of the experimentally induced infected full-thickness burn wounds in rats.


Articles in Press, Accepted Manuscript
Available Online from 29 September 2020
  • Receive Date: 23 August 2018
  • Revise Date: 29 September 2018
  • Accept Date: 20 October 2018