Veterinary Research Forum
Vet Res Forum

Use of ovine acellular peritoneal matrix combined with honey and ovine fetal ‎skin extract in the healing of full-thickness infected burn wounds in a rat ‎model

Document Type : Original Article

Authors

Behrouz Nikahval 1
Ahmad Oryan 2
Parastoo Memarian 1
Amir Kamali 2

1 Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

2 Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

https://doi.org/10.30466/vrf.2018.92422.2235
Abstract
Treatment of infected burn wounds remains a challenge in burn units. Silver-sulfadiazine (SSD) is the most commonly used topical antimicrobial agent in managing these wounds. We aimed to accelerate the healing of burn wounds by combined application of ovine acellular peritoneal matrix (OAPM), honey (H), and ovine fetal skin extract (OFSE). Sixty-four standardized burn wounds were created on the dorsum of 16 rats and were subsequently inoculated with Staphyloccocus aureus and Pseudomonas aeruginosa. After 48 hr, the wounds were surgically debrided and received either physiologic saline (control group) or SSD, OAPM+SSD, OAPM+H+SSD, OAPM+H+OFSE+SSD. The healing wounds were evaluated for size, bacterial counts, histopathology, and biomechanical properties on days 3, 7, 14, 21, and 28 after surgery. All treatments had effectively reduced the level of S. aureus and P. aeruginosa on wounds compared to the control group by day 3 and 7. The wounds treated with combined application of OAPM+H+OFSE+SSD demonstrated considerable inflammation reduction, fibroplasia, complete re-epithelialization, and wound contraction together with significantly lesser scar tissue formation. Treatment with OAPM+H+OFSE+SSD showed superior biomechanical properties of the healing wounds. The findings suggested that the synergistic effect of dressing the wounds with OAPM, H, and OFSE was a very effective approach in accelerating the healing process of the experimentally induced infected full-thickness burn wounds in rats.

Keywords

Acellular matrix
‎ Burn wounds
‎ Fetal skin extract
‎ Honey
‎ Wound healing
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Veterinary Research Forum
Volume 11, Issue 4
Autumn 2020
Pages 355-363

  • Receive Date 23 August 2018
  • Revise Date 29 September 2018
  • Accept Date 20 October 2018

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