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The effect of sodium selenite on apoptotic gene expression and development ‎of in vitro cultured mouse oocytes in comparison with in vivo obtained ‎oocytes

Document Type : Original Article

Authors

1 Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2 Department of Cellular and Molecular Biology, School of Biology, Damghan University, Damghan, Iran

3 Institute of Biological Sciences, ‎Damghan University, Damghan, Iran‎

4 Prevention of Metabolic Research Disorder Center, Research Institute for Endocrine Disorder, Shahid Beheshti University of Medical Science, ‎Tehran, Iran‎

Abstract

In vitro maturation (IVM) of oocytes is widely used in assisted reproduction technologies. The present study aimed to improve the in vitro oocyte maturation and its development through enriching the culture media with sodium selenite (SS). Moreover, the effects of SS on the expression of the oocytes apoptosis-related genes were assessed. In this study, male and female NMRI mice were used and after collecting their germinal vesicle (GV) oocytes, they were cultured with SS (experimental group) and without SS (control group). Collected metaphase II oocytes (MII) from the fallopian tube were considered as in vivo group.After in vitro culture, the oocytes were assessed in terms of nuclear maturation. The MII oocytes were inseminated and the development was examined until the blastocyst stage. Also, oocytes were subjected to the molecular analysis for evaluating the expression of BAX, BCL2, P53, and BAD genes using the real-time RT-PCR. The maturation rate was significantly increased in the SS supplemented group compared to the control one. The developmental rate of the embryos was significantly higher for both of the in vivo and SS supplemented groups rather than the control one, however, no significant difference was seen between these rates of the experimental and in vivo groups. Real-time RT-PCR did not show any significant differences in the expression of the apoptosis-related genes for all of the studied groups. The p53 gene was not expressed in any of groups. Sodium selenite improved the oocyte developmental competence but did not change the expression of the apoptosis-related genes in MII oocytes.

Keywords

References
  1. Szamatowicz M. Assisted reproductive technology in reproductive medicine - possibilities and limitations. Ginekol Pol 2016;87(12):820-823.
  2. Smitz J, Cortvrindt R. Oocyte in-vitro maturation and follicle culture: current clinical achievements and future directions. Hum Reprod 1999;14(suppl 1):145-161.
  3. Chian RC, Lim JH, Tan SL. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol 2004;16(3):211-219.
  4. Combelles CMH, Gupta S, Agarwal A. Could oxidative stress influence the in-vitro maturation of oocytes? Reprod Biomed Online 2009;18(6):864-880.
  5. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3(1):28. doi: 10.1186/1477-7827-3-28.
  6. Agarwal A, Said TM, Bedaiwy MA, et al. Oxidative stress in an assisted reproductive techniques setting. Fertil Steril 2006;86(3):503-512.
  7. Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis 2005;15(4):316-328.
  8. Rahimi G, Isachenko E, Sauer H, et al. Effect of different vitrification protocols for human ovarian tissue on reactive oxygen species and apoptosis. Reprod Fertil Dev 2003;15(6):343-349.
  9. Uhm SJ, Gupta MK, Yang JH, et al. Selenium improves the developmental ability and reduces the apoptosis in porcine parthenotes. Mol Reprod Dev 2007;74(11): 1386-1394.
  10. Elmore S. Apoptosis: a review of programmed cell death. Toxicol Pathol 2007;35(4):495-516.
  11. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat Rev Mol Cell Biol 2008;9(1):47-59.
  12. Burlacu A. Regulation of apoptosis by Bcl‐2 family proteins. J Cell Mol Med 2003;7(3):249-257.
  13. Al-Gubory KH, Fowler PA, Garrel C. The roles of cellular reactive oxygen species, oxidative stress and antioxidants in pregnancy outcomes. Int J Biochem Cell Biol 2010;42(10):1634-1650.
  14. Hatami S, Zavareh S, Salehnia M, et al. Total oxidative status of mouse vitrified pre-antral follicles with pre- antral follicles with pre-treatment of alpha lipoic acid. Iran Biomed J 2014;18(3):181-188.
  15. Hatami S, Zavareh S, Salehnia M, et al. Comparison of oxidative status of mouse pre‐antral follicles derived from vitrified whole ovarian tissue and vitrified pre‐antral follicles in the presence of alpha lipoic acid. J Obstet Gynaecol Res 2014;40(6):1680-1688.
  16. Hatami S, Zavareh S, Salehnia M, et al. The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries. Iran J Reprod Med 2014;12(1):57-64.
  17. Hosseinzadeh E, Zavareh S, Lashkarbolouki T. Anti-oxidant properties of coenzyme Q10-pretreated mouse pre-antral follicles derived from vitrified ovaries. J Obstet Gynaecol Res 2016;43(1):140-148.
  18. Kashka RH, Zavareh S, Lashkarbolouki T. Augmenting effect of vitrification on lipid peroxidation in mouse preantral follicle during cultivation: Modulation by co-enzyme Q10. Syst Biol Reprod Med 2016; 62(6):404-414.
  19. Rahnama A, Zavareh S, Ghorbanian MT, et al. The Effects of cAMP-elevating agents and alpha lipoic acid on in vitro maturation of mouse germinal vesicle oocytes. J Reprod Infertil 2013;14(4):173-183.
  20. Talebi A, Zavareh S, Kashani MH, et al. The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. J Assist Reprod Genet 2012;29(2):175-183.
  21. Gupta S, Sekhon L, Kim Y, et al. The role of oxidative stress and antioxidants in assisted reproduction. Curr Womens Health Rev 2010; 6(3):227-238.
  22. Halliwell B. Cell culture, oxidative stress, and anti-oxidants: avoiding pitfalls. Biomed J 2014;37(3):99-105.
  23. Saito Y, Yoshida Y, Akazawa T, et al. Cell death caused by selenium deficiency and protective effect of antioxidants. J Biol Chem 2003;278(41):39428-39434.
  24. Abedelahi A, Salehnia M, Allameh A, et al. Sodium selenite improves the in vitro follicular development by reducing the reactive oxygen species level and increasing the total antioxidant capacity and glutathione peroxide activity. Hum Reprod 2010; 25(4):977-985.
  25. Ma YM, Ibeanu G, Wang LY, et al. Selenium suppresses glutamate-induced cell death and prevents mitochondrial morphological dynamic alterations in hippocampal HT22 neuronal cells. BMC Neurosci 2017;18(1):15. doi: 10.1186/s12868-017-0337-4.
  26. Milner JA: Rationale and possible mechanisms by which selenium inhibits mammary cancer, in: Vitamins and minerals in the prevention and treatment of cancer. Fl, USA CRC Press, 2018,110-127.
  27. Vinceti M, Filippini T, Del Giovane C, et al. Selenium for preventing cancer. The Cochrane Library 2018. doi:10.1002/14651858.CD005195.pub4.
  28. Yeo JE, Kang SK. Selenium effectively inhibits ROS-mediated apoptotic neural precursor cell death in vitro and in vivo in traumatic brain injury. Biochim Biophys Acta 2007; 1772(11-12):1199-1210.
  29. Abedelahi A, Salehnia M, Allameh A. The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media. J Assist Reprod Genet 2008;25(9-10):483-488.
  30. Ghorbanmehr N, Salehnia M, Amooshahi M. The effects of sodium selenite on mitochondrial DNA copy number and reactive oxygen species levels of in vitro matured mouse oocytes. Cell J. 2018;20(3):396-402.
  31. Xiong X, Lan D, Li J, et al. Selenium supplementation during in vitro maturation enhances meiosis and developmental capacity of yak oocytes. Anim Sci J 2018;89(2):298-306.
  32. Alsalili M, Thornton S, Fleming S. The effect of the anaesthetic, propofol, on in-vitro oocyte maturation, fertilization and cleavage in mice. Hum Reprod 1997;12(6):1271-1274.
  33. Hayes MF, Sacco AG, Savoy-Moore RT, et al. Effect of general anesthesia on fertilization and cleavage of human oocytes in vitro. Fertil Steril 1987;48(6):975-981.
  34. Lazzaroni-Tealdi E, Barad DH, Albertini DF, et al. Oocyte scoring enhances embryo-scoring in predicting pregnancy chances with IVF where it counts most. PloS ONE 2015; 10(12): e0143632.doi:10.1371/journal. pone.0143632.
  35. Lasiene K, Vitkus A, Valaneiūte A, et al. Morphological criteria of oocyte quality. Medicina (Kaunas) 2009; 45(7):509-515.
  36. Zavareh S, Salehnia M, Saberivand A. Comparison of different vitrification procedures on developmental competence of mouse germinal vesicle oocytes in the presence or absence of cumulus cells. Int J Fertil Steril 2009;3(3):111-118.
  37. Xiao R, Qiao JT, Zhao HF, et al. Sodium selenite induces apoptosis in cultured cortical neurons with special concomitant changes in expression of the apoptosis-related genes. Neuro Toxicology 2006;27(4):478-484.
  38. Hwang JT, Kim YM, Surh YJ, et al. Selenium regulates cyclooxygenase-2 and extracellular signal-regulated kinase signaling pathways by activating AMP-activated protein kinase in colon cancer cells. Cancer Res 2006;66(20):10057-10063.
  39. Modarres SZ, Heidar Z, Foroozanfard F, et al. The effects of selenium supplementation on gene expression related to insulin and lipid in infertile polycystic ovary syndrome women candidate for in vitro fertilization: a randomized, double-blind, placebo-controlled trial. Biol Trace Elem Res 2018;183(2):218-225.
  40. Basini G, Tamanini C. Selenium stimulates estradiol production in bovine granulosa cells: possible involvement of nitric oxide. Domest Anim Endocrinol 2000;18(1):1-17.
  41. Melka MG, Rings F, Hölker M, et al. Expression of apoptosis regulatory genes and incidence of apoptosis in different morphological quality groups of in vitro‐produced bovine pre‐implantation embryos. Reprod Domest Anim 2010;45(5):915-921.
  42. Park SH, Cho HS, Yu IJ. Effect of bovine follicular fluid on reactive oxygen species and glutathione in oocytes, apoptosis and apoptosis‐related gene expression of in vitro‐produced blastocysts. Reprod Domest Anim 2014;49(3):370-377.
  43. Said RS, Nada AS, El-Demerdash E. Sodium selenite improves folliculogenesis in radiation-induced ovarian failure: a mechanistic approach. PloS ONE 2012; 7(12):e50928. doi:10.1371/journal.pone.0050928.
Veterinary Research Forum
Volume 11, Issue 4
December 2020
Pages 377-383
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History
  • Receive Date: 10 September 2018
  • Revise Date: 19 November 2018
  • Accept Date: 04 December 2018
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