Histopathological Assessment of Protective Effects of Selenium Nano-Particles on Rat Hepatocytes Exposed to Gamma Radiation

Document Type: Original Article


1 Department of Pathobiology, Faculty of Veterinary Medicine,Urmia University, Urmia, Iran,

2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Clinical Pathology,Faculty of college of Veterinary medicine,Urmia University, Urmia,Iran

4 Department of Medical Physics, Faculty of Medicine, Urmia University,Urmia, Iran

5 Department of Pathobiology, Faculty of Medicine, Virology Section, Tehran University, Tehran, Iran.



Gamma radiations are used in many medical and technical applications, but it is one of the most dangerous radiations and can be harmful for the body. The present study was designed to clarify the protective effects of the selenium supplementation as selenium nano- particles and selenite selenium in rat liver against gamma irradiation with different intensity of 2 and 8 GY. A total of 45 healthy male Wistar rats were randomly divided into 9 groups of five each. The radiation procedure was carried out in the Cobalt 60 equipment in Omid hospital in Urmia. The animals were simultaneously immobilized in a transparent acrylic plate and exposed to different intensity of 2 and 8 GY radiations on day 7th and 14th of experiment. After 72 hours after the last radiation, the animals euthanized and blood and liver tissue were collected. Histological analysis revealed radiation-induced hepatic injury in rats, which included vacuolated cytoplasm, liver necrosis, fibrosis, and vascular lesions followed by significant increases in alanine transaminase (ALT), alanine transaminase (AST), alkaline phosphatase (ALP) and Gamma-glutamyl transferase (GGT). Selenium nano particles has a more powerful antioxidant effect in comparison with selenite selenium and can effectively protect the liver cell against Gamma radiation at a dose of 8 GY.


Articles in Press, Accepted Manuscript
Available Online from 29 September 2020
  • Receive Date: 13 September 2018
  • Revise Date: 15 December 2018
  • Accept Date: 26 December 2018