Veterinary Research Forum
Vet Res Forum

Chemical composition and antibacterial effects of some essential oils ‎individually and in combination with sodium benzoate against methicillin-‎resistant Staphylococcus aureus and Yersinia enterocolitica

Document Type : Original Article

Authors

Maryam Ekhtelat 1, 2
Farzaneh Khalili Borujeni 1
Amir Siahpoosh 1, 2
Abdolghani Ameri 3

1 Medicinal Plant Research Center, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

2 Department of ‎Pharmacognosy, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Department of Drug and Food Control, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

https://doi.org/10.30466/vrf.2018.93152.2248
Abstract
Side effects of chemical preservatives and drug resistance have raised interest in use of herbal products. This study aimed to examine the chemical composition and antibacterial effects ofCuminum cyminum, Mentha spicata, and Mentha longifolia essential oils individually and in combination with sodium benzoate against methicillin-resistant Staphylococcus aureus and Yersinia enterocolitica. The essential oils were analyzed by gas chromatography-mass spectrometry. Disc diffusion and microdilution assays were used for in vitro antimicrobial screening. The main components were cumin aldehyde, carvone, and pulegone in C. cyminum, M. spicata, and M. longifolia essential oils, respectively. Antibacterial data analysis showed significant differences between different antibacterial effects of essential oils individually and in combination withsodium benzoate. In terms of individual effects, antibacterial effect of M. longifolia and C. cyminum essential oilswere the highest against methicillin-resistant S. aureus and Y. enterocolitica, respectively. The antibacterial effects of sodium benzoate combined with essential oils showed significant differences with the individual effect of sodium benzoate in most cases. The results indicated that the combined effect of these essential oils with sodium benzoate could reduce the use of sodium benzoate as an antimicrobial agent, which could decrease its possible side effects. Thus, for more significant effects, these essential oils could be combined with other agents for the preservation of drug and food products.

Keywords

Antibacterial effect
‎ Essential oil
‎ Methicillin-resistant S. aureus
‎ Sodium benzoate
‎ Yersinia enterocolitica
  1. Chouhan S, Sharma K, Guleria S. Antimicrobial activity of some essential oils—present status and future perspectives. Medicines (Basel). 2017;4(3):58, doi: 10.3390/medicines4030058.
  2. Burt S. Essential oils: their antibacterial properties and and potential applications in foods - a review. Int J Food Microbiol 2004;94(3):223-253.
  3. Mimica-Dukic N, Popovic M, Jakovljevic V, et al. Pharmacological studies of Mentha longifolia phenolic extracts. II. Hepatoprotective activity. Pharm Biol 1999;37(3):221-224.
  4. Gill AO, Holley RA. Disruption of Escherichia coli, Listeria monocytogenes and Lactobacillus sakei cellular membranes by plant oil aromatics. Int J Food Microbiol 2006; 108(1):1-9. doi: 10.1016/j.ijfoodmicro. 2005.10.009.
  5. Iacobellis NS, Lo Cantore P, Capasso F, et al. Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. J Agric Food Chem 2005;53(1):57-61.
  6. Škrovánková S, Mišurcová L, Machů L. Antioxidant activity and protecting health effects of common medicinal plants. Adv Food Nutr Res 2012;67:75-139.
  7. Wanner J, Bail S, Jirovetz L, et al. Chemical composition and antimicrobial activity of cumin oil (Cuminum cyminum, Apiaceae). Nat Prod Commun 2010; 5(9):1355-1358.
  8. Hussain AI, Anwar F, Shahid M, et al. Chemical composition, and antioxidant and antimicrobial activities of essential oil of spearmint (Mentha spicata L.) from Pakistan. J Essent Oil Res 2010;22(1):78-84.
  9. Mikaili P, Mojaverrostami S, Moloudizargari M, et al. Pharmacological and therapeutic effects of Mentha Longifolia L. and its main constituent, menthol. Anc Sci Life 2013;33(2):131-138.
  10. Boukarim C, Jaoude SA, Bahnam R, et al. Preservatives in liquid pharmaceutical preparations. Drug Test Anal 2009;1(3):146-148.
  11. Anand SP, Sati N. Artificial preservatives and their harmful effects: looking toward nature for safer alternatives. Int J Pharm Sci Res 2013;4(7):2496-2501.
  12. Klevens RM, Morrison MA, Nadle J, et al. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA 2007;298(15):1763-1771.
  13. Falcão JP, Brocchi M, Proença‐Módena JL, et al. Virulence characteristics and epidemiology of Yersinia enterocolitica and Yersiniae other than Y. pseudotuberculosis and Y. pestis isolated from water and sewage. J Appl Microbiol 2004; 96(6):1230-1236.
  14. Tavakoli HR, Mashak Z, Moradi B, et al. Antimicrobial activities of the combined use of Cuminum cyminum L. essential oil, nisin and storage temperature against Salmonella typhimurium and Staphylococcus aureus in vitro. Jundishapur J Microbiol 2015;8(4): e24838. doi: 10.5812/jjm.8(4)2015.24838.
  15. Jalali-Heravi M, Zekavat B, Sereshti H. Use of gas chromatography-mass spectrometry combined with resolution methods to characterize the essential oil components of Iranian cumin and caraway. J Chromatogr A 2007;1143(1-2):215-226.
  16. CLSI. Performance standards for antimicrobial susceptibility testing. 19th ed. Pennsylvania, USA: Clinical and Laboratory Standards Institute 2009; 29.
  17. CLSI. Method for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard. 8th ed. Pennsylvania, USA: Clinical and Laboratory Standards Institute 2009.17-18.
  18. Rohani SMR, Moradi M, Mehdizadeh T, et al. The effect of nisin and garlic (Allium sativum L.) essential oil separately and in combination on the growth of Listeria monocytogenes. LWT-Food Sci Technol 2011;44(10):2260-2265.
  19. Sadeghi E, Akhodzadeh-Basti A, Noori N, et al. Effect of Cuminum cyminum L. essential oil and Lactobacillus acidophilus (a probiotic) on Staphylococcus aureus during the manufacture, ripening and storage of white brined cheese. J Food Process Preserv 2013;37(5): 449-455.
  20. Lin KH, Yeh SY, Lin MY, et al. Major chemotypes and antioxidative activity of the leaf essential oils of Cinnamomum osmophloeum Kaneh. from a clonal orchard. Food Chem 2007;105(1):133-139.
  21. Asekun OT, Grierson DS, Afolayan AJ. Effects of drying methods on the quality and quantity of the essential oil of Mentha longifolia L. subsp. Capensis. Food Chem 2007;101(3):995-998.
  22. Snoussi M, Noumi E, Trabelsi N, et al. Mentha spicata essential oil: Chemical composition, antioxidant and antibacterial activities against planktonic and biofilm cultures of Vibrio spp. strains. Molecules 2015; 20(8):14402-14424.
  23. Kotan R, Kordali S, Cakir A. Screening of antibacterial activities of twenty-one oxygenated monoterpenes. Z Naturforsch C J Biosci 2007;62(7-8):507-513.
  24. Zengin H, Baysal AH. Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy. Molecules 2014;19(11):17773-17798.
  25. Kumar P, Mishra S, Malik A, et al. Insecticidal properties of Mentha species: A review. Ind Crops Prod 2011;34(1):802-817.
  26. Mandal S, DebMandal M, Saha K, et al. In vitro antibacterial activity of three Indian spices against methicillin-resistant Staphylococcus aureus. Oman Med J 2011;26(5):319-323.
  27. Abu-Shanab B, Adwan G, Jarrar N, et al. Antibacterial activity of four plant extracts used in Palestine in folkloric medicine against methicillin-resistant Staphylococcus aureus. Turk J Biol 2006;30(4):195-198.
  28. Bokaeian M, Shiri Y, Bazi S, et al. Antibacterial activities of Cuminum cyminum Linn. essential oil against multi-drug resistant Escherichia coli. Int J Infect 2014;1(1). e18739, doi: 10.17795/iji-18739.
  29. Leistner L, Gorris LGM. Food preservation by hurdle technology. Trends in Food Sci Technol 1995;6:41-46.
  30. Moosavy MH, Akhondzaseh Basti A, Misaghi A, et al. Effect of Zataria multiflora Boiss. essential oil and nisin on Salmonella typhimurium and Staphylococcus aureus in a food model system and on the bacterial cell membranes. Food Res Int 2008;41(10):1050-1057.
  31. Mann CM, Cox SD, Markham JL. The outer membrane of Pseudomonas aeruginosa NCTC 6749 contributes to its tolerance to the essential oil of Melaleuca alternifolia (tea tree oil). Lett Appl Microbiol 2000;30(4):294-297.
  32. Stefanini MB, Figueiredo RO, Ming LC, et al. Antimicrobial activity of the essential oils of some spice herbs.Acta Horticulturae 2003; doi: 10.17660/Acta Hortic.2003.597.30.
  33. Shahbazi Y. Chemical composition and in vitro antibacterial activity of Mentha spicata essential oil against common food-borne pathogenic bacteria. J Pathog 2015;2015. doi: 10.1155/2015/916305.
  34. Nikšić H, Kovač-Bešović E, Makarević E, et al. Chemical composition, antimicrobial and antioxidant properties of Mentha longifolia (L.) Huds. essential oil. J Health Sci 2012;2(3):192-200.
  35. Kalemba D, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003;10(10):813-829.
  36. Zengin N, Yüzbaşioğlu D, Unal F, et al. The evaluation of the genotoxicity of two food preservatives: Sodium benzoate and potassium benzoate. Food Chem Toxicol 2011;49(4):763-769.
  37. Beezhold BL, Johnston CS, Nochta KA. Sodium benzoate-rich beverage consumption is associated with increased reporting of ADHD symptoms in college students: a pilot investigation. J Atten Disord 2014;18(3):236-241.
Veterinary Research Forum
Volume 11, Issue 4
Autumn 2020
Pages 333-338

  • Receive Date 06 September 2018
  • Revise Date 05 December 2018
  • Accept Date 29 December 2018

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