Document Type : Original Article
Authors
1 Department of Anatomical Sciences, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
2 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3 Animal Core Facility, Reproductive Biomedicine Research Center, Royan Institute For Biotechnology, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
Abstract
Several studies have indicated that feeding female animal with fatty acids could result in alteration of offspring sex ratio; however, there is little information available regarding the effect of feeding male animal with fatty acids on sex ratio of offspring. The aim of current study was to investigate the effect of paternal feeding with n-3 and n-6 polyunsaturated fatty acids (PUFAs) on sperm characteristics and sex ratio of offspring in mice. Male mice received 2.50% of palm oil (control), fish oil (n-3 PUFAs) or sunflower oil (n-6 PUFAs) for two months. Sperm kinematics and viability were assessed using computer-assisted sperm analyzer and eosin-Y staining, respectively. Additionally, female mice were randomly introduced to males of three experimental groups to determine reproductive parameters including litter size, conception rate and sex ratio. Fish oil enhanced sperm concentration, motility and viability, whereas sunflower oil decreased sperm concentration. Nevertheless, progressive motility, velocity, linearity and straightness of sperms were not affected by source of fatty acids. Although supplementation with fish oil resulted in male-biased sex ratio, palm and sunflower oils did not impact sex ratio. Besides, source of fatty acids failed to influence conception rate and litter size. In conclusion, the present study provided evidence for the impact of paternal fish oil consumption on sex ratio of offspring for the first time. Moreover, it showed beneficial effect of n-3 PUFAs and adverse effects of n-6 PUFAs on seminal parameters.
Keywords
- Russo GL. Dietary n-6 and n-3 polyunsaturated fatty acids: from biochemistry to clinical implications in cardiovascular prevention. Biochem Pharmacol 2009; 77(6): 937-946.
- Gharagozlou F, Youssefi R, Akbarinejad V. Effects of diets supplemented by fish oil on sex ratio of pups in bitch. Vet Res Forum 2016;7(2):105-110.
- Wall R, Ross RP, Fitzgerald GF, et al. Fatty acids from fish: the anti-inflammatory potential of long-chain omega-3 fatty acids. Nutr Rev 2010; 68(5):280-289.
- Calder PC. Fatty acids and inflammation: the cutting edge between food and pharma. Eur J Pharmacol 2011; 668(Suppl 1): S50-S58.
- Rosenfeld CS, Roberts RM. Maternal diet and other factors affecting offspring sex ratio: a review. Biol Reprod 2004; 71(14):1063-1070.
- Grant VJ, Chamley LW. Can mammalian mothers influence the sex of their offspring peri-conceptually? Reproduction 2010;140(3):425-433.
- Akbarinejad V, Niasari-Naslaji A, Mahmoudzadeh H, et al. Effects of diets enriched in different sources of fatty acids on reproductive performance of Zel sheep. Iran J Vet Res 2012;13(4):310-316.
- Emadi SR, Rezaei A, Bolourchi M, et al. Administration of estradiol benzoate before to insemination could skew secondary sex ratio toward males in Holstein dairy cows. Domest Anim Endocrinol 2014;48:110-118.
- Gharagozlou F, Youssefi R, Vojgani M, et al. Androgen receptor blockade using flutamide skewed sex ratio of litters in mice. Vet Res Forum 2016; 7(2):169-172.
- Rosenfeld CS, Grimm KM, Livingston KA, et al. Striking variation in the sex ratio of pups born to mice according to whether maternal diet is high in fat or carbohydrate. Proc Natl Acad Sci USA 2003; 100(8): 4628-4632.
- Fountain ED, Mao J, Whyte JJ, et al. Effects of diets enriched in omega-3 and omega-6 polyunsaturated fatty acids and offspring sex-ratio and maternal behavior in mice. Biol Reprod 2008; 78(2): 211-217.
- Green MP, Spate LD, Parks TE, et al. Nutritional skewing of conceptus sex in sheep: effects of a maternal diet enriched in rumen-protected poly-unsaturated fatty acids (PUFA). Reprod Biol Endocrinol 2008; 6: 21. doi: 10.1186/1477-7827-6-21.
- Wathes DC, Abayasekara DRE, Aitken RJ. Poly-unsaturated fatty acids in male and female reproduction. Biol Reprod 2007;77(2):190-201.
- Yan L, Bai XL, Fang ZF, et al. Effect of different dietary omega-3/omega-6 fatty acid ratios on reproduction in male rats. Lipids Health Dis 2013; 12-33. doi: 10.1186/1476-511X-12-33.
- Esmaeili V, Shahverdi AH, Moghadasian MH, et al. Dietary fatty acids affect semen quality: a review. Andrology 2015; 3(3):450-461.
- Feng Y, Ding Y, Liu J, et al. Effects of dietary omega-3/omega-6 fatty acid ratios on reproduction in the young breeder rooster. BMC Vet Res 2015; 11, 73. doi:10.1186/s12917-015-0394-9.
- Ferramosca A, Moscatelli N, Di Giacomo M, et al. Dietary fatty acids influence sperm quality and function. Andrology 2017; 5(3):423-430.
- Gomendio M, Malo AF, Soler AJ, et al. Male fertility and sex ratio at birth in red deer. Science 2006; 314(5804):1445-1447.
- Mossman JA, Slate J, Birkhead TR, et al. Sperm speed is associated with sex bias of siblings in a human population. Asian J Androl 2013; 15(1):152-154.
- Ramaswamy S, Weinbauer GF. Endocrine control of spermatogenesis: Role of FSH and LH/ testosterone. Spermatogenesis 2015; 4(2):e996025. doi: 10.1080/ 21565562.2014.996025.
- Luo L, Chen H, Zirkin BR. Leydig cell aging: steroidogenic acute regulatory protein (StAR) and cholesterol side-chain cleavage enzyme. J Androl 2001; 22(1):149-156.
- Zaima N, Kinoshita S, Hieda N, et al. Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidyl-choline in testicular interstitium. Biochem Biophys Rep 2016; 7:259-265.
- Fan YY, Zhan Y, Aukema HM, et al. Proapoptotic effects of dietary (n-3) fatty acids are enhanced in colonocytes of manganese-dependent superoxide dismutase knockout mice. J Nutr 2009; 139(7):1328-1332.
- Sakai C, Ishida M, Ohba H, et al. Fish oil omega-3 polyunsaturated fatty acids attenuate oxidative stress-induced DNA damage in vascular endothelial cells. PLoS ONE 2017; 12(11):e0187934 doi:10.1371/journal.pone.0187934.
- Ciftci O, Cetin A, Aydin M, et al. Fish oil, contained in eicosapentaenoic acid and docosahexaenoic acid, attenuates testicular and spermatological damage induced by cisplatin in rats. Andrologia 2014; 46(10):1161-1168.
- Risso AL, Pellegrino FJ, Corrada Y, et al. Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs. J Nutr Sci 2017; 11: 6:e48 doi: 10.1017/ jns.2017.29.
- Bansal AK, Bilaspuri GS. Impacts of oxidative stress and antioxidants on semen functions. Vet Med Int 2011; 2011: 686137 doi: 10.4061/2011/686137.
- Agarwal A, Virk G, Ong C, et al. Effect of oxidative stress on male reproduction. World J Mens Health 2014; 32(1): 1-17.
- Amaral A, Lourenço B, Marques M, et al. Mitochondria functionality and sperm quality. Reproduction 2013; 146(5):R163-R174.
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