Document Type : Original Article
Authors
- Mohamed Mohamed Ali 1
- Salwa Mahmoud Helmy 1
- Hanan Ali Fahmy 2
- Haitham Elaadli 3
- Ibrahim Elsayed Eldesoukey 4
1 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University 33516, Egypt
2 Department of Biotechnology, Animal Health Research Institute (ARC), Giza 12618, Egypt.
3 Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Alexandria University, Alexandria 22758, Egypt
4 Department of Bacteriology, Mycology and Immunology Faculty of Veterinary Medicine Kafrelsheikh University
Abstract
Staphylococcus aureus (S. aureus) is one of the most common etiological agents of mastitis worldwide. The current study aimed to determine the prevalence and antimicrobial resistance patterns of S. aureus in mastitic milk samples collected from several camel farms located in Matrouh Governorate, Egypt. In that context, a total of 200 mastitic camel milk samples were evaluated for S. aureus using a conventional culture-based method and a molecular-based approach. The antibiotic susceptibility testing of S. aureus isolates was investigated using the procedures of disc diffusion and agar dilution methods. The antibiotic resistance genes were identified by polymerase chain reaction using particular primers. Of the samples screened, 60 (30%) were verified for S. aureus. S. aureus isolates displayed the highest level of resistance against Piperacillin-tazobactam (55%) followed by Trimethoprim-sulphamethoxazole (45%) and Amoxycillin (40%). Fifty % of the isolates were multidrug-resistant. The antimicrobial resistant determinants such as methicillin-resistant gene (mecA), beta-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in percentage of 100%, 100%, 95%, 90% and 20% of the isolates, respectively. In conclusion, the occurrence of multiple drug resistant S. aureus as a causative agent of clinical camel mastitis represents a major veterinary issue and also a potential public health hazard. Furthermore, the obtained findings unequivocally demonstrate the necessity for appropriate use of antibiotics in camel farms in Egypt and further molecular methodologies to identify the complete genetic profile for the recovered antimicrobial resistant S. aureus isolates.
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