Veterinary Research Forum
Vet Res Forum

Effect of Gundelia tournefortii extract on diabetic gastropathy: involvement of inflammation, apoptosis, oxidative stress, and histopathology

Document Type : Original Article

Authors

Muhammet Bahaeddin Dörtbudak 1
Uğur Şeker 2
Muhammed Demircioğlu 3
Ismail Demircioğlu 4

1 Department of Pathology, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye

2 Department of Histology and Embryology, Faculty of Medicine, Mardin Artuklu University, Mardin, Türkiye

3 Department of Histology and Embryology, Institute of Health Sciences, Dicle University, Diyarbakir, Türkiye

4 Department of Anatomy, Faculty of Veterinary Medicine, Harran University, Şanliurfa, Türkiye

https://doi.org/10.30466/vrf.2024.2027690.4249
Abstract
In this study, the effect of Gundelia tournefortii (GT) extract against diabetic gastropathy was investigated by pathological methods. The animal groups were designed as the control, diabetes, diabetes + GT50, diabetes + GT100, and diabetes + GT200 groups. No treatment was applied to the control group. The other groups received 45.00 mg kg-1 streptozotocin intraperitoneally on the experimental day. The treatment groups were also given 50.00, 100, and 200 mg kg-1 of GT extract daily by gavage for 21 days. Tissues were stained with Hematoxylin and Eosin for histopathological examination. Immunohistochemical staining was performed to reveal the presence of inflammation (tumor necrosis factor alpha), apoptosis (cysteine aspartate specific proteases-3), and oxidative stress (heat shock protein-27). Histopathological examination revealed no pathological lesion in the control group. In the diabetes group, mucosal tissue damage, and vascular and inflammatory changes were observed. In the treatment groups, GT decreased histopathological findings in parallel with the dose increase. Immunohistochemical examination revealed no immunopositivity in the control group, while severe immunopositivity was observed in the diabetes groups in terms of inflammation, apoptosis, and oxidative stress. In the treatment groups, there was a decrease in the severity of immunopositivity’s depending on the dose increase. As a result of this study, which has not been done before, GT was found to have a protective effect against gastropathy, being an important complication of diabetes, and this study is thus an important reference point for future research and promises new hope for the patients.

Keywords

Cysteine aspartate specific proteases-3
Diabetes
Gundelia tournefortii
Heat shock protein-27
Tumor necrosis factor alpha

Subjects

  1. Cole JB, Florez JC. Genetics of diabetes mellitus and diabetes complications. Nat Rev Nephrol 2020; 16(7): 377-390.
  2. Harding JL, Pavkov ME, Magliano DJ, et al. Global trends in diabetes complications: a review of current evidence. Diabetologia 2019; 62(1): 3-16.
  3. Krishnan B, Babu S, Walker J, et al. Gastrointestinal complications of diabetes mellitus. World J Diabetes 2013; 4(3): 51-63.
  4. Zawada AE, Moszak M, Skrzypczak D, et al. Gastrointestinal complications in patients with diabetes mellitus. Adv Clin Exp Med 2018; 27(4): 567-572.
  5. Volpe CMO, Villar-Delfino PH, Dos Anjos PMF, et al. Cellular death, reactive oxygen species (ROS) and diabetic complications. Cell Death Dis 2018; 9(2): 119. doi: 10.1038/s41419-017-0135-z.
  6. Farrugia Histologic changes in diabetic gastroparesis. Gastroenterol Clin North Am 2015; 44(1): 31-38.
  7. Du YT, Rayner CK, Jones KL, et al. Gastrointestinal symptoms in diabetes: prevalence, assessment, pathogenesis, and management. Diabetes Care 2018; 41(3): 627-637.
  8. Mehrzadeh M, Ziaeezadeh F, Pasdaran A, et al. A review of the ethnobotany, biological activity, and phyto-chemistry of the plants in the Gundelia genus. Chem Biodivers 2024; 21(3): e202301932. doi: 10.1002/cbdv.202301932.
  9. Amer J, Salhab A, Jaradat N, et al. Gundelia tournefortii inhibits hepatocellular carcinoma progression by lowering gene expression of the cell cycle and hepatocyte proliferation in immunodeficient mice. Biomed Pharmacother 2022; 156: 113885. doi: 10.1016/j.biopha.2022.113885.
  10. Enteshari Najafabadi M, Roozbeh Nasiraie L, Ghasemi Pirblouti A, et al. Biological and prebiotic activities of polysaccharides from Taraxacum officinale FH Wigg., Cichorium intybus L., and Gundelia tournefortii L. J Food Meas Charact 2024; 18(2): 1412-1421.
  11. Abu‑Lafi S, Rayan B, Kadan S, et al. Anticancer activity and phytochemical composition of wild Gundelia tournefortii. Oncol Lett 2019; 17(1): 713-717.
  12. Vidyasagar A, Wilson NA, Djamali A. Heat shock protein 27 (HSP27): biomarker of disease and therapeutic target. Fibrogenesis Tissue Repair 2012; 5(1): 7. doi: 10.1186/1755-1536-5-7.
  13. Quarta S, Massaro M, Carluccio MA, et al. An exploratory critical review on TNF-α as a potential inflammatory biomarker responsive to dietary intervention with bioactive foods and derived products. Foods 2022; 11(16): 2524. doi: 10.3390/foods11162524.
  14. Ward TH, Cummings J, Dean E, et al. Biomarkers of apoptosis. Br J Cancer 2008; 99(6): 841-846.
  15. Kurt S, Seker U, Yazlik MO, et al. Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. J Res Pharm 2023; 27(2): 652-664.
  16. Demircioğlu M, Dörtbudak MB. Protective effect of Gundelia tournefortii extract on spleen tissue in experimental type I diabetes in rats. J Med Dent Inves 2023; 4(1): e230339. doi: 10.5577/ jomdi.e230339.
  17. Salem MB, Elzallat M, Mohammed DM, et al. Cornu aspersum mucin attenuates indomethacins-induced gastric ulcers in mice via alleviating oxidative stress and inflammation. Heliyon 2023; 9(5): e15677. doi: 10.1016/j.heliyon.2023.e15677.
  18. Şahin A, Sakat MS, Kılıç K, et al. The protective effect of Naringenin against ovalbumin-induced allergic rhinitis in rats. Eur Arch Otorhinolaryngol 2021; 278(12): 4839-4846.
  19. Çelebi A, Dörtbudak MB, Keskinrüzgar A, et al. The therapeutic effect of bovine colostrum on 5-Fluorouracil-Induced oral mucositis in rats. J Stomatol Oral Maxillofac Surg 2022; 123(6): e682-e686.
  20. Intagliata N, Koch KL. Gastroparesis in type 2 diabetes mellitus: prevalence, etiology, diagnosis, and treat-ment. Curr Gastroenterol Rep 2007; 9(4): 270-279.
  21. Kojecky V, Bernatek J, Horowitz M, et al. Prevalence and determinants of delayed gastric emptying in hospitalised Type 2 diabetic patients. World J Gastroenterol 2008; 14(10): 1564-1569.
  22. Solanki ND, Bhavsar SK, Pandya DT. Role of phytotherapy in diabetic neuropathy and neurodegeneration: from pathogenesis to treatment. J Phytopharmacol 2018; 7(2): 152-161.
  23. Harberson J, Thomas RM, Harbison SP, et al. Gastric neuromuscular pathology in gastroparesis: analysis of full-thickness antral biopsies. Dig Dis Sci 2010; 55(2): 359-370.
  24. Heidari F, Rabizadeh S, Mansournia MA, et al. Inflammatory, oxidative stress and anti-oxidative markers in patients with endometrial carcinoma and diabetes. Cytokine 2019; 120: 186-190.
  25. Vador N, Jagtap AG, Damle A. Vulnerability of gastric mucosa in diabetic rats, ıts pathogenesis and amelioration by Cuminum cyminum. Indian J Pharm Sci 2012; 74(5): 387-396.
  26. Sidarala V, Kowluru A. The regulatory roles of mitogen-activated protein kinase (MAPK) pathways in health and diabetes: lessons learned from the pancreatic β-cell. Recent Pat Endocr Metab Immune Drug Discov 2017; 10(2): 76-84.
  27. Elshazly SM, Abd El Motteleb DM, Ibrahim IAAH. Hesperidin protects against stress induced gastric ulcer through regulation of peroxisome proliferator activator receptor gamma in diabetic rats. Chem Biol Interact 2018; 291: 153-161.
  28. Fouad AA, Al-Sultan AI, Yacoubi MT, et al. Ameliorative effects of telmisartan in diabetic rats with indomethacin-induced gastric ulceration. Eur J Pharmacol 2010; 637(1-3): 162-170.
  29. Khaleel EF, Mostafa DG, Abdel-Aleem GA. Gastroprotective effect of flavonoid quercetin and coenzyme Q10 in indomethacin-induced gastric ulcers in normal and diabetic rats. IOSR J Dent Med Sci 2015; 14(12): 58-71.
  30. Turkyilmaz IB, Bayrak BB, Sacan O, et al. Zinc supplementation restores altered biochemical parameters in stomach tissue of STZ diabetic rats. Biol Trace Elem Res 2021; 199(6): 2259-2265.
  31. Yilmaz-Ozden T, Kurt-Sirin O, Tunali S, et al. Ameliorative effect of vanadium on oxidative stress in stomach tissue of diabetic rats. Bosn J Basic Med Sci 2014; 14(2): 105-109.
  32. Owu DU, Obembe AO, Nwokocha CR, et al. Gastric ulceration in diabetes mellitus: protective role of vitamin C. ISRN Gastroenterol 2012; 2012: 362805. doi: 10.5402/2012/362805.
  33. Ashrafi Jigheh Z, Ghorbani Haghjo A, Argani H, et al. Empagliflozin alleviates renal inflammation and oxidative stress in streptozotocin-induced diabetic rats partly by repressing HMGB1-TLR4 receptor axis. Iran J Basic Med Sci 2019; 22(4): 384-390.
  34. Rasoulinejad SA, Akbari A, Nasiri K. Interaction of miR-146a-5p with oxidative stress and inflammation in complications of type 2 diabetes mellitus in male rats: anti-oxidant and anti-inflammatory protection strategies in type 2 diabetic retinopathy. Iran J Basic Med Sci 2021; 24(8): 1078-1086.
  35. Dörtbudak MY, Çadırcı MŞ, Karakılçık AZ. Effects of vitamin E and selenium on erythrocyte and platelet indices and pancreatic histopathology in experimental diabetes [Turkish]. Harran Üniv Tıp Fak Derg 2013; 10(2): 54-59.
  36. Mohammadi G, Zangeneh MM, Rashidi K, et al. Evaluation of nephroprotective and antidiabetic effects of Gundelia tournefortii aqueous extract on diabetic nephropathy in male mice. Res J Pharmacogn 2018; 5(4): 65-73.
Veterinary Research Forum
Volume 16, Issue 3
March 2025
Pages 133-139

  • Receive Date 29 April 2024
  • Revise Date 07 June 2024
  • Accept Date 29 June 2024

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