Veterinary Research Forum
Vet Res Forum

Does co-supplementation of zinc, manganese, and copper affect plasma testosterone, sperm quality, and anti-oxidant enzyme activities of ram?

Document Type : Original Article

Authors

Sona Zargari 1
Armin Towhidi 1
Kamran Rezayazdi 1
John Patrick kastelic 2

1 Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Faculty of Veterinary Medicine, University of Calgary, Calgary, Canada

https://doi.org/10.30466/vrf.2024.2025806.4222
Abstract
Adding trace minerals to animal diets has a positive impact on reproductive performance. This study aimed to investigate whether supplementing zinc, manganese, and copper affects the testosterone level, sperm quality, and anti-oxidant enzyme activities in rams. Ten mature Afshari rams (2.50 years old; weighing 100 ± 3.29 kg) were fed a nutritionally adequate diet for 11 weeks, half of which receiving a sulfated zinc, manganese, and copper in their daily concentrates (supplemented group), while the other half served as a control group receiving no mineral in the concentrates. Ejaculate volume, as well as sperm concentration, motility, viability, morphology, and membrane integrity were assessed. From week five onwards, all parameters were significantly higher in the supplemented group. Additionally, the percentage of abnormal sperm was lower, anti-oxidant enzymes activities in the seminal plasma were improved, and plasma testosterone concentration was higher in the supplemented group compared to the control group. However, alkaline phosphatase activity was not significantly different. Furthermore, seminal plasma concentrations of copper, zinc, and manganese at the end of the study were higher in the supplemented group compared to pre-treatment levels, while these parameters were decreased in the control group. Overall, co-supplementing copper, zinc, and manganese in rams maintained the sperm quantity and quality, as well as seminal plasma anti-oxidant capacity, emphasizing the importance of studied elements in ram reproduction.

Keywords

Anti-oxidant capacity
Copper
Manganese
Ram
Zinc

Subjects

  1. Kendall NR, McMullen S, Green A, et al. The effect of a zinc, cobalt and selenium soluble glass bolus on trace element status and semen quality of ram lambs. Anim Reprod Sci 2000; 62(4): 277-283.
  2. Arangasamy A, Krishnaiah MV, Manohar N, et al. Cryoprotective role of organic Zn and Cu supplementation in goats (Capra hircus) diet. Cryobiology 2018; 81: 117-124.
  3. Venkata Krishnaiah M, Arangasamy A, Selvaraju S, et al. Organic Zn and Cu interaction impact on sexual behaviour, semen characteristics, hormones and spermatozoal gene expression in bucks (Capra hircus). Theriogenology 2019; 130: 130-139.
  4. Chen MT, Cheng GW, Lin CC, et al. Effects of acute manganese chloride exposure on lipid peroxidation and alteration of trace metals in rat brain. Biol Trace Elem Res 2006; 110(2): 163-178.
  5. Magnus O, Brekke I, Abyholm T, et al. Effects of manganese and other divalent cations on progressive motility of human sperm. Arch Androl 1990; 24(2): 159-166.
  6. Narasimhaiah M, Arunachalam A, Sellappan S, et al. Organic zinc and copper supplementation on anti-oxidant protective mechanism and their correlation with sperm functional characteristics in goats. Reprod Domest Anim 2018; 53(3): 644-654.
  7. Ebisch IM, Pierik FH, DE Jong FH, et al. Does folic acid and zinc sulphate intervention affect endocrine parameters and sperm characteristics in men? Int J Androl 2006; 29(2): 339-345.
  8. Rahman HU, Qureshi MS, Khan RU. Influence of dietary zinc on semen traits and seminal plasma antioxidant enzymes and trace minerals of Beetal bucks. Reprod Domest Anim 2014; 49(6): 1004-1007.
  9. Park CS, Kim BG. In vitro solubility of copper(II) sulfateand dicopper chloride trihydroxide for pigs. Asian-Australas J Anim Sci 2016; 29(11): 1608-1615.
  10. Caramalac LS, Netto AS, Martins PGMA, et al. Effects of hydroxychloride sources of copper, zinc, and manganese on measures of supplement intake, mineral status, and pre- and postweaning performance of beef calves. J Anim Sci 2017: 95(4): 1739-1750.
  11. Byrne L, Murphy RA. Relative bioavailability of trace minerals in production animal nutrition: a review. Animals (Basel) 2022; 12(15): 1981. doi: 10.3390/ ani12151981.
  12. Ott RS, Memon MA. Breeding soundness examinations of rams and bucks, a review. Theriogenology 1980; 13(2): 155-164.
  13. Nutrient Requirements of Small Ruminants (NRC): Sheep, Goats, Cervids, and New World Camelids. 7th National Academy Press, Washington, DC, USA 2007.
  14. Evans G, Maxwell WMC. Salamons' artificial insemination of sheep and goats. 2nd Sydney, Australia: Butterworths 1987; 105-128.
  15. Jeyendran RS, Van der Ven HH, Zaneveld LJ. The hypoosmotic swelling test: an update. Arch Androl 1992; 29(2): 105-116.
  16. Tavilani H, Goodarzi MT, Vaisi-Raygani A, et al. Activity of antioxidant enzymes in seminal plasma and their relationship with lipid peroxidation of spermatozoa. Int Braz J Urol 2008; 34(4): 485-491.
  17. Richeson JT, Kegley EB. Effect of supplemental trace minerals from injection on health and performance of highly stressed, newly received beef heifers. Pro Anim Sci 2011; 27(5): 461-466.
  18. Rowe MP, Powell JG, Kegley EB, et al. Effect of supplemental tracemineral source on bull semen quality. Pro Anim Sci 2014; 30(1): 68-73.
  19. Zargari S, Masoumi R, Rostami B, et al. Morphological and biochemical characteristics of Afshari and Afshari×Booroola Merino cross bred rams (cross-continental cross breeding) semen before and after cryopreservation. Small Rumin Res 2016; 139: 26-29.
  20. SAS User's Guide: Statistics.Version 9.1. SAS Institute, Inc.,Cary, North Carolina. USA 2002.
  21. Hatfield PG, Swenson CK, Kott RW, et al. Zinc and copper status in ewes supplemented with sulfate-and amino acid-complexed forms of zinc and copper. J Anim Sci 2001; 79(1): 261-266.
  22. Mason The biochemical pathogenesis of molybdenum-induced copper deficiency syndromes in ruminants: towards the final chapter. Ir Vet J 1990; 43, 18-21.
  23. van Kuijk S, Swiegers P, Han Y. Hydroxychloride trace minerals improve apparent total tract nutrient digestibility in Bonsmara beef cattle. Livest Sci 2022; 256: 104820. doi: 10.1016/j.livsci.2021.104820.
  24. MacDonald RS. The role of zinc in growth and cell proliferation. J Nutr 2000; 130(5S Suppl): 1500S-1508S.
  25. Cheah Y, Yang W. Functions of essential nutrition for high quality spermatogenesis. Adv Biosci Biotechnol 2011; 2(4): 182-197.
  26. Hernández-Meléndez J, Salem AZM, Sánchez-Dávila F, et al. Effect of copper and zinc supplementation on growth, blood serum copper and zinc levels, scrotal circumference and semen quality in growing male Boer × Nubian bucks. Life Sci J 2015; 12(2s): 108-112.
  27. Majumder A, Thakur M, Bhakat M, et al. Effect of dietary copper and zinc supplementation on semen quality of Murrah bulls. Indian J Anim Res 2020; 54(10): 1260-1264.
  28. Eghbali M, Alavi-Shoushtari SM, Rezaii SA. Effects of copper and superoxide dismutase content of seminal plasma on buffalo semen characteristics. Pak J Biol Sci 2008; 11(15): 1964-1968.
  29. Tarun A, Mani V, Bhakat M, et al. Effect of dietary supplementation of manganese, chromium and cobalt on semen qualities in Sahiwal bulls. Indian J Anim Res 2020; 54(9): 1109-1114.
  30. Bettger WJ, O'Dell BL. A critical physiological role of zinc in the structure and function of biomembranes. Life Sci 1981; 28(13): 1425-1438.
  31. Roy B, Baghel R, Mohanty T, et al. Zinc and male reproduction in domestic animals: a review. Indian J Anim Nutr 2013; 30(4): 339-350.
  32. Máchal L, Chládek G, Straková E. Copper, phosphorus and calcium in bovine blood and seminal plasma in relation to semen quality. J Anim Feed Sci 2002; 11(3): 425-435.
  33. Studer JM, Schweer WP, Gabler NK, et al. Functions of manganese in reproduction. Anim Reprod Sci 2022; 238: 106924. doi: 10.1016/j.anireprosci.2022.106924.
  34. Ahsan U, Kamran Z, Raza I, et al. Role of selenium in male reproduction - a review. Anim Reprod Sci 2014; 146(1-2): 55-62.
  35. Jockenhövel F, Bals‐Pratsch M, Bertram HP, et al. Seminal lead and copper in fertile and infertile men. Andrologia 1990; 22(6): 503-511.
  36. Chia SE, Ong CN, Chua LH, et al. Comparison of zinc concentrations in blood and seminal plasma and the various sperm parameters between fertile and infertile men. J Androl 2000; 21(1): 53-57.
  37. Tajaddini S, Ebrahimi S, Behnam B, et al. Antioxidant effect of manganese on the testis structure and sperm parameters of formalin‐treated mice. Andrologia 2014; 46(3): 246-253.
  38. Page CM, Van Emon ML, Murphy TW, et al. Effects of zinc source and dietary concentration on serum zinc concentrations, growth performance, wool and reproductive characteristics in developing rams. Animal 2020; 14(3): 520-528.
  39. Gowda NK, Ramana JV, Prasad CS, et al. Micronutrient content of certain tropical conventional and unconventional feed resources of Southern India. Trop Anim Health Prod 2004; 36(1): 77-94.
  40. Khan RU. Antioxidants and poultry semen quality. J World's Poult Sci 2011; 67(2): 297-308.
  41. Goel A, Dani V, Dhawan DK. Protective effects of zinc on lipid peroxidation, antioxidant enzymes and hepatic histoarchitecture in chlorpyrifos-induced toxicity. Chem Biol Interact 2005; 156(2-3): 131-140.
  42. Lee B, Pine M, Johnson L, et al. Manganese acts centrally to activate reproductive hormone secretion and pubertal development in male rats. Reprod Toxicol 2006; 22(4): 580-585.
  43. Wilde D. Influence of macro and micro minerals in the peri-parturient period on fertility in dairy cattle. Anim Reprod Sci 2006; 96(3-4): 240-249.
  44. Shinde AK, Sankhyan SK, Kumar D, et al. Effects of supplementation of copper and zinc on nutrient intake, utilization, blood profile, wool yield and semen quality of Malpura rams. Indian J Small Rumin 2012; 18(2): 191-197.
Veterinary Research Forum
Volume 16, Issue 3
March 2025
Pages 167-174

  • Receive Date 01 April 2024
  • Revise Date 05 July 2024
  • Accept Date 14 August 2024

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