Veterinary Research Forum
Vet Res Forum

Provitamin A carotenoid (β-cryptoxanthin) ameliorated testicular ischemia-reperfusion injury in mature rats

Document Type : Original Article

Authors

Kianoush Mohammadnejad 1
Rahim Mohammadi 1
Ali Soleimanzadeh 2
Ali Shalizar 3
Farshid Sarrafzadeh-Rezaei 1

1 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2025.2046637.4559
Abstract
Buildup of reactive oxygen species during testicular torsion causes oxidative stress and ischemia-reperfusion (I/R) injury in testis. The purpose of this study was to investigate influence of β-cryptoxanthin (BCX) on I/R injury in testicular torsion/detorsion in mature rats. Thirty mature male Wistar rats were divided into five groups of six animals each, including sham group: In this group, midline incision of the scrotum was performed and the testicles were taken out for 2 hr with a 720-degree rotation, I/R group: In this group, midline incision of the scrotum was performed and the testicles were taken out and undergone ischemia for 2 hr with a 720-degree rotation, I/R/Oil group: In this group, a midline scrotum cut was performed, the testicles were taken out, ischemia was created for 2 hr with a 720-degree rotation, and at the end of ischemia 100 µL of corn oil (BCX solvent) was injected intraperitoneally, I/R/BCX10 group: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (10.00 µg kg-1) at the end of ischemia, and I/R/BCX40: The same as I/R/Oil group, as well as intraperitoneal administration of 100 µL of BCX (40.00 µg kg-1) at the end of ischemia. Evaluations were based on histopathological and spermatological parameters and oxidative stress assessments. Histopathological spermatological and oxidative stress parameters values obtained from I/R/BCX40 were significantly different from those of other groups (p < 0.05). It could be concluded that BCX could ameliorate testicular injuries in acute testicular torsion/detorsion in mature rats.

Keywords

β-cryptoxanthin
Carotenoid
Ischemia-reperfusion injury
Provitamins
Testis

Subjects

  1. Wang S, Tang H, Zhang J, et al. Comparison of clinical features and outcomes between two age groups of cryptorchidism testicular torsion in children: a retrospective study in single center. Front Pediatr 2024; 12: 1296222. doi: 10.3389/fped.2024.1296222.
  2. Jacobsen FM, Rudlang TM, Fode M, et al. The impact of testicular torsion on testicular function. World J Mens Health 2020; 38(3): 298-307.
  3. Deng QF, Yang C, Mao C, et al. Clinical and hematological analysis of testicular torsion in children. Front Pediatr 2024;12: 1399349. doi: 10.3389/fped. 2024.1399349.
  4. Parlaktas BS, Atilgan D, Ozyurt H, et al. The biochemical effects of ischemia-reperfusion injury in the ipsilateral and contralateral testes of rats and the protective role of melatonin. Asian J Androl 2014; 16(2): 314-318.
  5. Abbasoğlu L, Kalaz EB, Soluk-Tekkeşin M, et al. Beneficial effects of taurine and carnosine in experimental ischemia/reperfusion injury in testis. Pediatr Surg Int 2012; 28(11): 1125-1131.
  6. Olugbodi JO, Samaila K, Lawal B, et al. Computational and preclinical evidence of anti-ischemic properties of L-carnitine-rich supplement via stimulation of anti-inflammatory and antioxidant events in testicular torsed rats. Oxid Med Cell Longev 2021; 2021: 5543340. doi: 10.1155/2021/5543340.
  7. Roshangar L, Rad JS, Afsordeh K. Maternal tamoxifen treatment alters oocyte differentiation in the neonatal rats: inhibition of oocyte development and decreased folliculogenesis. J Obstet Gynaecol Res 2010; 36(2): 224-231.
  8. Meštrović J, Drmić-Hofman I, Pogorelić Z, et al. Beneficial effect of nifedipine on testicular torsion-detorsion injury in rats. Urology 2014; 84(5): 1194-1198.
  9. Volpe CMO, Villar-Delfino PH, Dos Anjos PMF, et al. Cellular death, reactive oxygen species (ROS) and diabetic complications. Cell Death Dis 2018; 9(2): 119. doi: 10.1038/s41419-017-0135-z.
  10. Karaguzel E, Kadihasanoglu M, Kutlu O. Mechanisms of testicular torsion and potential protective agents. Nat Rev Urol 2014; 11(7): 391-399.
  11. Lapenna D, Ciofani G, Pierdomenico SD, et al. Reaction conditions affecting the relationship between thio-barbituric acid reactivity and lipid peroxides in human plasma. Free Radic Biol Med 2001; 31(3): 331-335.
  12. Daenen K, Andries A, Mekahli D, et al. Oxidative stress in chronic kidney disease. Pediatr Nephrol 2019; 34(6): 975-991.
  13. Baghban A, Mohammadi R, Mohammadi V, et al. Effects of intraperitoneal administration of α-tocopherol acetate on ischemia-reperfusion injury in ovaries in female cats of animal house shelter undergoing elective ovariohysterectomy. Iran J Vet Surg 2024; 19(2): 89-96.
  14. Conti M, Morand PC, Levillain P, et al. Improved fluorometric determination of malonaldehyde. Clin Chem 1991; 37(7): 1273-1275.
  15. Arena S, Iacona R, Antonuccio P, et al. Medical perspective in testicular ischemia-reperfusion injury. Exp Ther Med 2017; 13(5): 2115-2122.
  16. Azqueta A, Collins AR. Carotenoids and DNA damage. Mutat Res 2012; 733(1-2): 4-13.
  17. Leary S, Underwood W, Anthony R, et al. AVMA guidelines for the euthanasia of animals: 2020 edition. Available at: www.avma.org/sites/default/files/2020-02/Guidelines-on-Euthanasia-2020.pdf. Accessed April 24, 2025.
  18. Raisi A, Davoodi F, Mohammadi R. Protective effects of rosmarinic acid on testicular torsion-detorsion in an animal model. Iran J Vet Surg 2022; 17(2): 80-86.
  19. Yariravesh P, Mohammadi R, Asri-Resaei S. PEG-based clotrimazole microparticles improved ischemia-reperfusion injury in rat testicular torsion and detorsion model: evaluation of tissue oxidative stress. Iran J Vet Surg 2024; 19(1): 1-7.
  20. Mostahsan Z, Azizi S, Soleimanzadeh A, et al. The protective roles of Mito-TEMPO on testicular ischemia-reperfusion injury based on biochemical and histopathological evidences in mice. Iran J Vet Surg 2024; 19(2): 97-105.
  21. Vakili-Sadeghi E, Najafpour A, Mohammadi R. Protective effects of propolis on ischemia-reperfusion injury in a rat testicular torsion and detorsion model. Vet Res Forum 2023; 14(7): 389-395.
  22. World health organization, laboratory manual for the examination and processing of human semen. 5th ed. New York, USA: WHO Library Cataloguing-in-Publication Data; 2010.
  23. Mogilner JG, Lurie M, Coran AG, et al. Effect of diclofenac on germ cell apoptosis following testicular ischemia-reperfusion injury in a rat. Pediatr Surg Int 2006; 22(1): 99-105.
  24. Burri BJ, La Frano MR, Zhu C. Absorption, metabolism, and functions of β-cryptoxanthin. Nutr Rev 2016; 74(2): 69-82.
  25. Raju AB, Challa SR, Akula A, et al. Evaluation of oxidant and anti-oxidant balance in experimentally induced testicular injury by ischemia reperfusion in rats. Eur J Gen Med 2011; 8(2): 117-121.
  26. Bignotto L, Rocha J, Sepodes B, et al. Anti-inflammatory effect of lycopene on carrageenan-induced paw oedema and hepatic ischaemia-reperfusion in the rat. Br J Nutr 2009; 102(1): 126-133.
  27. Li Y, Xue F, Xu SZ, et al. Lycopene protects bone marrow mesenchymal stem cells against ischemia-induced apoptosis in vitro. Eur Rev Med Pharmacol Sci 2014; 18(11): 1625-1631.
  28. Ozkan E, Akyüz C, Dulundu E, et al. Protective effects of lycopene on cerulein-induced experimental acute pancreatitis in rats. J Surg Res 2012; 176(1): 232-238.
  29. Kaya C, Karabulut R, Turkyilmaz Z, et al. Lycopene has reduced renal damage histopathologically and biochemically in experimental renal ischemia-reperfusion injury. Ren Fail 2015; 37(8): 1390-1395.
  30. Breinholt V, Lauridsen ST, Daneshvar B, et al. Dose-response effects of lycopene on selected drug-metabolizing and antioxidant enzymes in the rat. Cancer Lett 2000; 154(2): 201-210.
  31. Celik O, Turkoz Y, Hascalik S, et al. The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary. Eur J Obstet Gynecol Reprod Biol 2004; 117(2): 183-188.
  32. Zhang F, Shi D, Wang X, et al. β-cryptoxanthin alleviates myocardial ischaemia/reperfusion injury by inhibiting NF-κB-mediated inflammatory signalling in rats. Arch Physiol Biochem 2022; 128(4): 1128-1135.
  33. Codoñer-Franch P, Muñiz P, Gasco E, et al. Effect of a diet supplemented with alpha-tocopherol and beta- carotene on ATP and antioxidant levels after hepatic ischemia-reperfusion. J Clin Biochem Nutr 2008; 43(1): 13-18.
  34. Vardi N, Parlakpinar H, Ozturk F, et al. Potent protective effect of apricot and beta-carotene on methotrexate-induced intestinal oxidative damage in rats. Food Chem Toxicol 2008; 46(9): 3015-3022.
  35. Güzel M, Sönmez MF, Baştuğ O, et al. Effectiveness of lycopene on experimental testicular torsion. J Pediatr Surg 2016; 51(7): 1187-1191.
Veterinary Research Forum
Volume 16, Issue 5
May 2025
Pages 277-284

  • Receive Date 24 November 2024
  • Revise Date 18 January 2025
  • Accept Date 01 February 2025

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