Normal values of pulse wave Doppler echocardiography in healthy Ghezel sheep

Document Type : Original Article

Authors

1 Department of Veterinary Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Large Animal Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

4 Department of Cardiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Abstract
Echocardiography is a noninvasive, highly sensitive method for evaluating heart disease in veterinary medicine, crucial for measuring cardiac structures and functions. Despite its importance, limited data exist on sheep, particularly the Ghezel breed, a significant breed in Iran. This study aimed to establish normal echocardiographic values for Ghezel sheep, providing reference data for both clinical and research applications. We evaluated 15 healthy Ghezel sheep with a mean age of 16.50 ± 1.80 months and a mean weight of 42.69 ± 4.73 kg. Comprehensive clinical exams, biochemical and hematological tests and color Doppler echocardiography were performed. Descriptive results from standard right and left parasternal views in both longitudinal and transverse planes were obtained and compared to existing studies. The study successfully established pulse wave Doppler reference values for the Ghezel breed. These findings could be used in diagnosing heart disease in Ghezel sheep and would be beneficial in future research particularly in sheep used as an animal model for translation in human cardiac studies.

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  1. Youssef M, Nassif M, El-khodery S, et al. Assessment of cardiac indices using M-mode echocardiography in horse with tachycardia. Mansoura VetMed J 2019; 20(1): 9. doi: 10.21608/mvmj.2019.01.101.
  2. Grenacher PA, Schwarzwald CC. Assessment of left ventricular size and function in horses using anatomical M-mode echocardiography. J Vet Cardiol 2010; 12(2): 111-121.
  3. Boutet BG, Gordon SG, De Solis CN, et al. Feasibility and clinical utility of transvenous intracardiac echocardiography in conscious, sedated horses. J Vet Cardiol 2022; 42: 15-22.
  4. Abbott JA, MacLean HN. Comparison of Doppler-derived peak aortic velocities obtained from subcostal and apical transducer sites in healthy dogs. Vet Radiol Ultrasound 2003; 44(6): 695-698.
  5. Morita T, Nakamura K, Osuga T, et al. Interventricular inflow time difference assessed by dual pulsed-wave Doppler echocardiography in dogs with myxomatous mitral valve disease. J Vet Cardiol 2024; 51: 24-34.
  6. Osuga T, Kuroda K, Morita T, et al. Prognostic value of left atrial stiffness estimated using echocardiography in dogs with myxomatous mitral valve disease. J Vet Cardiol 2023; 45: 15-26.
  7. Silva AC, Muzzi RA, Oberlender G, et al. Longitudinal strain and strain rate by two-dimensional speckle tracking in non-sedated healthy cats. Res Vet Sci 2013; 95(3): 1175-1180.
  8. Karpenko LY, Kozitsyna AI, Bakhta AA, et al. Prognostic criteria for assessing of hypertrophic cardiomyopathy in cats [Russian]. Issues Legal Regulation Vet Med 2022; (1). doi: 10.52419/ issn2782-6252.2022.1.44.
  9. Leroux AA, Moonen ML, Farnir F, et al. Two-dimensional and M-mode echocardiographic reference values in healthy adult Saanen goats. Vet Rec 2012; 170(6): 154. doi: 10.1136/vr.100192.
  10. Leroux AA, Farnir F, Moonen ML, et al. Repeatability, variability and reference values of pulsed wave Doppler echocardiographic measurements in healthy Saanen goats. BMC Vet Res 2012; 8: 190. doi: 10.1186/1746-6148-8-190.
  11. Hallowell GD, Potter TJ, Bowen IM. Methods and normal values for echocardiography in adult dairy cattle. J Vet Cardiol 2007; 9(2): 91-98.
  12. Buczinski S. Cardiovascular ultrasonography in cattle. Vet Clin North Am Food Anim Pract 2009; 25(3): 611-632.
  13. Poser H, Semplicini L, Benedictis GM, et al. Two-dimensional, M-mode and Doppler-derived echo-cardiographic parameters in sedated healthy growing female sheep. Lab Anim 2013; 47(3): 194-202.
  14. Kirberger RM, van den Berg JS. Pulsed wave Doppler echocardiographic evaluation of intracardiac blood flow in normal sheep. Res Vet Sci 1993; 55(2): 189-194.
  15. Aissi A. B-mode real time ultrasonographic imaging of the heart in sheep. Vet World 2011; 4(10): 467-469.
  16. Penninck D, d’Anjou MA. Atlas of Small Animal Ultrasonography. 2nd Ames, USA: Wiley-Blackwell 2015; 111-182.
  17. Locatelli P, Olea FD, De Lorenzi A, et al. Reference values for echocardiographic parameters and indexes of left ventricular function in healthy, young adult sheep used in translational research: comparison with standardized values in humans. Int J Clin Exp Med 2011; 4(4): 258-264.
  18. Hallowell GD, Potter TJ, Bowen IM. Reliability of quantitative echocardiography in adult sheep and goats. BMC Vet Res 2012; 8: 181. doi: 10.1186/1746-6148-8-181.
  19. Olsson K, Hansson A, Hydbring E, et al. A serial study of heart function during pregnancy, lactation and the dry period in dairy goats using echocardiography. Exp Physiol 2001; 86(1): 93-99.
  20. Blissitt KJ, Bonagura JD. Pulsed wave Doppler echocardiography in normal horses. Equine Vet J Suppl 1995; (19): 38-46.
  21. Caivano D, Corda A, Rishniw M, et al. Transthoracic M-mode echocardiographic assessment of pulmonary vein-to-pulmonary artery ratio in healthy horses. PLoS One 2019;14(8): e0221154. doi: 10.1371/journal. pone.0221154.
  22. Mattoon JS, Sellon RK, Berry CR. Small Animal Diagnostic Ultrasound. 4th St. Louis, USA: Elsevier 2020; 230-354.
  23. Sadeghian Chaleshtori S, Mokber Dezfouli MR, Vajhi A, et al. Right parasternal echocardiographic reference values in the healthy male Iranian Shall sheep. Vet Med Sci 2024; 10(5). e1535. doi: 10.1002/vms3.1535.
  24. Roustaei A, Hashemi-Asl SM, Dalir-Naghadeh B, et al. Two-dimensional and M-mode echocardiographic parameters in Ghezel sheep. Vet Res Forum 2021; 12(3): 349-355.
  25. Yadegari M. Normal echocardiographic findings in Lori-Bakhtiari sheep. HVM Bioflux 2014;6(2): 45-48.
Volume 16, Issue 4
April 2025
Pages 229-234

  • Receive Date 01 July 2024
  • Revise Date 02 October 2024
  • Accept Date 05 November 2024