Veterinary Research Forum
Vet Res Forum

Conventional and molecular identification of Iranian Clostridia species associated with animal infection

Document Type : Original Article

Authors

Lida Abdolmohammadi khiav 1
Anahita Emadi 2
Maryam Dadar 3

1 Department of Anaerobic Vaccine Research and Production, Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

2 Department of Bacterial vaccines Quality Control, Razi vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

3 Razi vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran

https://doi.org/10.30466/vrf.2024.2035437.4361
Abstract
Clostridial disease causes severe economic losses in livestock by rapidly killing ruminants. Therefore, implementing effective control approaches to prevent this fatal disease is importance. The causative agent of this disease is Clostridium spp. Accurate identification of this microorganism is crucial for effectively managing clostridial diseases in farm. There are conventional methods for detecting the disease, including microbiological and biochemical tests, many of these tests are time-consuming and exhibit low sensitivity. So, this study aims to use conventional and molecular approaches to identify Iranian isolates associated with animal infections. To achieve this, 61 samples were collected from1984 to 2024 and cultured on liver media, and subsequently subjected to microbiological and biochemical tests. For molecular identification, the DNA of isolates was extracted, and the isolates were confirmed by polymerase chain reaction (PCR) using specific primers. The results of the conventional analysis revealed that all Iranian isolates were identified as C. perfringens and its type determined by PCR assay. According to our findings, C. perfringens type A is the most prevalent strain in Iran, which predominantly found in ostriches and bird samples, followed by type D. This study underscores the presence of C. perfringens types across variety hosts and geographic locations in Iran. In conclusion, the combining conventional methods with PCR helps reliably detecting Clostridium spp. This information holds the potential to significantly contribute to the development of preventive strategies against clostridial diseases in Iran.

Keywords

Biochemical tests
Clostridium perfringens
Iranian isolate
PCR

Subjects

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Veterinary Research Forum
Volume 16, Issue 6
June 2025
Pages 345-352

  • Receive Date 13 July 2024
  • Revise Date 25 December 2024
  • Accept Date 28 December 2024

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