Veterinary Research Forum
Vet Res Forum

Molecular detection of the Escherichia coli heme-utilization gene A virulence factor in E. coli isolated from the feces of horses in Sumbawa island, Indonesia

Document Type : Original Article

Authors

Kholik Kholik 1
Akhmad Sukri 2
Katty Hendriana Priscilia Riwu 1
Ieke Wulan Ayu 3
Ika Nurani Dewi 2

1 Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Mataram, Indonesia

2 Department of Biology Education, Faculty of Science and Engineering, Universitas Pendidikan Mandalika, Indonesia

3 Department of Argotechnology, Faculty of Agriculture, Universitas Samawa, Sumbawa, Indonesia

https://doi.org/10.30466/vrf.2024.2039823.4421
Abstract
The transmission of Escherichia coli (E. coli) containing virulent genes from animals to humans and the environment poses significant public health challenges. This study aimed to detect the virulence factor of the E. coli heme-utilization gene A (chuA) in E. coli isolated from the feces of apparently healthy horses in the island of Sumbawa, Indonesia. The study utilized 52 fecal samples from a total horse population of 283, calculated using the disease detection formula. Fresh feces were collected immediately after excretion and placed in buffered peptone water for subsequent analysis. The samples were then isolated on eosin methylene blue media and identified using biochemical tests. Identified E. coli strains were further examined for detecting the chuA gene using polymerase chain reaction techniques. The E. coli was successfully isolated and identified in 11 (21.15%) of the 52 collected fecal samples. Polymerase chain reaction analysis detected the chuA gene in 8 (15.38%) E. coli isolates at 279 bp on gel electrophoresis. The close interaction between horses and humans in the island of Sumbawa,  Indonesia, may facilitate the spread of E. coli. Thus, surveillance is needed to employ a One Health approach to monitor E. coli strains encoding the chuA gene and other virulence factors to control their dissemination.

Keywords

chuA
Escherichia coli
Horse
Indonesia
Virulence factors

Subjects

  1. BPS-Statistics of Nusa Tenggara Barat Province. Sum-bawa Horse Livestock Population, Sumbawa Regency (Tail), 2018-2020. Available at: https://sumbawakab. bps.go.id/indicator/24/146/1/populasi-ternak-kuda-sumbawa-kabupaten-sumbawa.html. Accessed Aug 04, 2024.
  2. Reshadi P, Heydari F, Ghanbarpour R, et al. Molecular characterization and antimicrobial resistance of potentially human‐pathogenic Escherichia coli strains isolated from riding horses. BMC Vet Res 2021; 17(1): 131. doi: 10.1186/s12917-021-02832-x.
  3. Lyimo B, Buza J, Subbiah M, et al. Comparison of antibiotic resistant Escherichia coli obtained from drinking water sources in northern Tanzania: a cross-sectional study. BMC Microbiol 2016; 16: 254. doi: 10.1186/s12866-016-0870-9.
  4. Lawin A, Abdul-Rahman ZFA. Identification and molecular detection of chuA gene of pathogenic Escherichia coli. PJMHS 2020;14(2): 1181-1184.
  5. Derakhshan S, Farhadifar F, Roshani D, et al. Study on the presence of resistant diarrheagenic pathotypes in Escherichia coli isolated from patients with urinary tract infection. Gastroenterol Hepatol Bed Bench 2019; 12(4): 348-357.
  6. Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2000; 66(10): 4555-4558.
  7. Saitz W, Montero DA, Pardo M, et al. Characterization of adherent-invasive Escherichia coli (AIEC) outer membrane proteins provides potential molecular markers to screen putative AIEC strains. Int J Mol Sci 2022; 23(16): 9005. doi: 10.3390/ijms23169005.
  8. Kholik K, Sukri A, Riwu KHP, et al. Detection of the chuA gene encoding the invasive enterohemorrhagic species Escherichia coli 0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia. Open Vet J 2024 ;14(4): 1051-1058.
  9. Carlos C, Pires MM, Stoppe NC, et al. Escherichia coli phylogenetic group determination and its application in the identification of the major animal source of fecal contamination. BMC Microbiol 2010; 10: 161. doi: 10.1186/1471-2180-10-161.
  10. Hoffmann H, Hornef MW, Schubert S, et al. Distribution of the outer membrane haem receptor protein chuA in environmental and human isolates of Escherichia coli. Int J Med Microbiol 2001; 291(3): 227-230.
  11. Thrusfield M. Veterinary epidemiology 3rd Oxford, UK: Blackwell Science Ltd 2005; 238-240.
  12. Vandepitte J, Verhaegen J. Engbaek K, et al‎. Basic laboratory procedures in clinical bacteriology. 2nd Geneva, Swiss: World Health Organization 2003: 37-59.
  13. Sadeq JN, Al-Husseiny SH, Al Muhana BMM, et al. Isolation and identification of Escherichia coli O157:H7 from houseflies (Musca domestica L) at cattle barns in Al-Qadisiyah Province, Iraq. Vet Integr Sci 2023; 22(1): 65-72.
  14. Kholik, Munawaroh M, Saputra MRI, et al. Antibiotic resistance in Escherichia coli isolated from feces of Bali cattle with reproductive disorders. Jurnal Biodjati 2021; 6(2): 303-311.
  15. Abdul-Razzaq MS, Abdul-Lateef LA. Molecular phylogeny of Escherichia coli isolated from clinical samples in Hilla, Iraq. Afr J Biotechnol 2011; 10(70): 15783-15787.
  16. Kholik K, Srianto P, Aulanniam A, et al. Characterization and phylogenetics of beta-lactamase Temoneira gene in Escherichia coli of the Bali cattle on Lombok island, Indonesia. Iraqi J Vet Sci 2023; 37(2): 487-493.
  17. Shahid SS, Yousif MG. Prevalence of chuA gene virulence factor in Escherichia Coli isolated from clinical samples in AL-Diwaniyah province. Int J Health Sci 2022; 6(S5): 2610-2618.
  18. Duangurai T, Rungruengkitkul A, Kong-Ngoen T, et al. Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra Wildlife Sanctuary, Thailand. Vet World 2022; 15(12): 2800-2809.
  19. Ayaz ND, Gencay YE, Erol I. Prevalence and molecular characterization of sorbitol fermenting and non-fermenting Escherichia coli O157:H7(+)/H7(-) isolated from cattle at slaughterhouse and slaughterhouse wastewater. IntJ Food Microbiolo 2014; 174: 31-38.
  20. Spurbeck RR, Dinh PC Jr, Walk ST, et al. Escherichia coli isolates that carry vat, fyuA, chuA, and yfcV efficiently colonize the urinary tract. Infect Immun 2012; 80(12): 4115-4122.
  21. Yousif AA, Hussein MA. Prevalence and molecular detection of intimin (eaeA) virulence gene in coli O157:H7 in calves. Res J Vet Pract 2015; 3(3): 47-52.
  22. Messerer M, Fischer W, Schubert S. Investigation of horizontal gene transfer of pathogenicity islands in Escherichia coli using next-generation sequencing. PloS One 2017; 12(7): e0179880. doi: 10.1371/journal. pone.0179880.
  23. Bach SJ, McAllister TA, Baah J, et al. Persistence of Escherichia coli O157:H7 in barley silage: effect of a bacterial inoculant. J Appl Microbiol 2022; 93(2): 288-294.
  24. AbuOun M, Jones H, Stubberfield E, et al. A genomic epidemiological study shows that prevalence of anti-microbial resistance in Enterobacterales is associated with the livestock host, as well as antimicrobial usage. Microb Geno 2021; 7(10): 000630. doi: 10.1099/ mgen.0.000630.
Veterinary Research Forum
Volume 16, Issue 6
June 2025
Pages 325-330

  • Receive Date 02 September 2024
  • Revise Date 05 October 2024
  • Accept Date 06 October 2024

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