Veterinary Research Forum
Vet Res Forum

Inhibitory effect of cell-free supernatants of Pedicoccous acidilactici and Latilactobacillus sakei/Staphylococcus xylosus in combination with ethylenediaminetetraacetic acid against Escherichia coli O157:H7 strains

Document Type : Original Article

Authors

Gökhan Kürşad İncili 1
Osman İrfan İlhak 2
Mehmet Çalıcıoğlu 1

1 Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Fırat University, Elaziğ, Türkiye

2 Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Balıkesir University, Balıkesir, Türkiye

https://doi.org/10.30466/vrf.2025.2035776.4368
Abstract
The current research was carried out to evaluate in vitro anti-microbial properties of the cell-free supernatants (CFSs) derived from Pediococcus acidilactici (PA) and Latilactobacillus sakei/ Staphylococcus xylosus (LS) against Escherichia coli O157:H7 American Type Culture Collection strains (35150, 43894, and 43985). For this purpose, the diameters of zone of inhibition of the CFSs against E. coli O157:H7 strains were measured. In addition, a time-kill assay was conducted to determine the inhibitory effect of the CFSs alone or in combination with ethylenediaminetetraacetic acid (EDTA) during incubation at 37.00 ˚C for 24 hr. In the time-kill assay, E. coli O157:H7 was subjected to three concentrations of CFSs (1.00, 5.00, and 10.00%) and EDTA (0.02 M) in tryptic soy broth and the E. coli O157:H7 count was determined at 0, 6, and 24 hr intervals. The CFS of LS had a lower pH and higher titratable acidity compared to the PA. The CFS of LS displayed higher zones of inhibition than the CFS of PA against E. coli O157:H7 American Type Culture Collection 35150 and 43894 strains. The concentrations of 5.00 and 10.00% CFSs in combination with EDTA provided a 5.00 log10 decline in E. coli O157:H7 count over a 24-hr period. The results of this study indicated that the combination of CFSs (5.00 and 10.00%) and EDTA (0.02 M) exhibited an enhanced anti-bacterial effect against E. coli O157:H7 strains, which are substantial foodborne pathogenic bacteria.

Keywords

Anti-microbial activity
Cell-free supernatant
Disk diffusion
Escherichia coli O157:H7

Subjects

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Veterinary Research Forum
Volume 16, Issue 8
August 2025
Pages 439-446

  • Receive Date 16 July 2024
  • Revise Date 30 January 2025
  • Accept Date 18 February 2025

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