Veterinary Research Forum
Vet Res Forum

Molecular detection and phylogenetic analysis of feline panleukopenia virus in domestic cat population of Mizoram state, India

Document Type : Original Article

Authors

Theresa Zochampuii
Tridib Kumar Rajkhowa
Kiran Jayappa

Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Central Agricultural University, Aizawl, India

https://doi.org/10.30466/vrf.2025.2039338.4410
Abstract
The most significant infectious disease that affects cats is thought to be feline panleukopenia, also known as Cat distemper. Despite its epidemiological status, few literatures are available regarding the clinic-pathological aspect of the disease and about the molecular epidemiology of the circulating feline panleukopenia virus (FPV) in India. This study gives a comprehensive insight into the prevalence, pathology and diagnosis of FPV in cat population of Mizoram. Twenty-six cats that died of clinical disease suspected of FPV were subjected to a thorough pathological examination followed by molecular diagnosis. The FPV infection was confirmed in 12 out of the 26 cats by polymerase chain reaction assay targeting the VP2 gene of FPV. The phylogenetic analysis based on the full VP2 gene of FPV has demonstrated close genetic affinity of FPV strains circulating in Mizoram with the isolates from Thailand (MW589472), Italy (MZ508524) and China (OR727315). The analysis of the VP2-deduced amino acid sequence revealed two distinct mutations, S179T and I401V, exclusively identified in isolates from this particular study.

Keywords

FPV
Mizoram
Molecular surveillance
Pathology
Polymerase chain reaction

Subjects

1.     Barrs VR. Feline panleukopenia: a re-emergent disease. Vet Clin North Am Small Anim Pract 2019; 49(4): 651-670.
2.     Kruse BD, Unterer S, Horlacher K, et al. Prognostic factors in cats with feline panleukopenia. J Vet Intern Med 2010; 24(6): 1271-1276.
3.     Steinel A, Parrish CR, Bloom ME, et al. Parvovirus infections in wild carnivores. J Wildl Dis 2001; 37(3): 594-607.
4.     Cotmore SF, Agbandje-McKenna M, Chiorini JA, et al. The family Parvoviridae. Arch Virol 2014; 159(5): 1239-1247.
5.     Csiza CK, De Lahunta A, Scott FW, et al. Pathogenesis of feline panleukopenia virus in susceptible newborn kittens II. Pathology and immunofluorescence. Infect Immun 1971; 3(6): 838-846.
6.     Kilham L, Margolis G, Colby ED. Cerebellar ataxia and its congenital transmission in cats by feline panleukopenia virus. J Am Vet Med Assoc 1971; 158(6): 888-901.
7.     Bancroft JD, Stevens A. Theory and practice of histological techniques. 3rd ed. London, UK: Churchill Livingstone 1990; 83-92.
8.     Green MR, Sambrook J. Molecular cloning: A laboratory Manual, 4th ed. New York, USA: Cold Spring Harbor Laboratory Press 2012; 1254-1259.
9.     Zhang Q, Niu J, Yi S, et al. Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus. Arch Virol 2019; 164(11): 2761-2768.
10. Yang S, Wang S, Feng H, et al. Isolation and characterization of feline panleukopenia virus from a diarrheic monkey. Vet Microbiol 2010; 143(2-4): 155-159.
11. Kumar S, Stecher G, Li M, et al. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018; 35(6): 1547-1549.
12. Martin DP, Murrell B, Golden M, et al. RDP4: Detection and analysis of recombination patterns in virus genomes. Virus Evol 2015; 1(1): vev003. doi: 10.1093/ ve/vev003.
13. Bouckaert R, Vaughan TG, Barido-Sottani J, et al. BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis. PLoS Comput Biol 2019; 15(4): e1006650. doi: 10.1371/journal.pcbi.1006650.
14. Rambaut A, Drummond AJ, Xie D, et al. Posterior summarization in Bayesian phylogenetics using Tracer 1.7. Syst Biol 2018; 67(5): 901-904.
15. Garigliany M, Gilliaux G, Jolly S, et al. Feline panleukopenia virus in cerebral neurons of young and adult cats. BMC Vet Res 2016; 12:28. doi: 10.1186/ s12917-016-0657-0.
16. Pirarat N, Kaewamatawong T, Techangamsuwan S. A retrospective immunohistochemistry study on feline panleukopenia virus, induced enteritis, in cats. Thai J Vet Med 2002; 32(4): 71-78.
17. Tucciarone CM, Franzo G, Legnardi M, et al. Genetic insights into feline parvovirus: evaluation of viral evolutionary patterns and association between phylogeny and clinical variables. Viruses 2021; 13(6): 1033. doi: 10.3390/v13061033.
18. Decaro N, Desario C, Miccolupo A, et al. Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis. J Gen Virol 2008; 89(Pt 9): 2290-2298.
Veterinary Research Forum
Volume 16, Issue 10
October 2025
Pages 585-590

  • Receive Date 22 August 2024
  • Revise Date 20 January 2025
  • Accept Date 18 February 2025

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