Veterinary Research Forum
Vet Res Forum

Cathelicidin-1 as a potential biomarker for anestrus detection in cows

Document Type : Original Article

Authors

Ambika Sharma 1
Ashish Kumar 2
Arvind Kumar Tripathi 3
Vikas Sachan 4
Pawanjit Singh 1

1 Department of Veterinary Biochemistry, College of Veterinary Science, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, India

2 Department of Pharmacology, All India Institute of Medical Sciences, Rishikesh, India

3 Department of Veterinary Medicine, College of Veterinary Science, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, India

4 Department of Veterinary Gynaecology and Obstetrics, College of Veterinary Science, Uttar Pradesh Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, Mathura, India

https://doi.org/10.30466/vrf.2025.2043046.4488
Abstract
Cathelicidin-1, an antimicrobial peptide, has garnered attention for its potential role as a biomarker in detecting anestrus in cows, providing insights into bovine reproductive health. This study aimed to analyse cathelicidin-1 within the urinary proteome and evaluate its effectiveness as a diagnostic tool for anestrus in cows. The study employed tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry techniques to identify and characterize cathelicidin-1 in the context of anestrus in cows. The analysis confirmed the presence and distinct profile of cathelicidin-1, highlighting its significance in reflecting the physiological and pathophysiological states associated with anestrus. Cathelicidin-1 as a promising diagnostic biomarker for anestrus in cows could revolutionize bovine reproductive management by offering more precise and advanced detection methods compared to the traditional, time-consuming, and sometimes inaccurate approaches, like behavioral observation or hormonal assays. If cathelicidin-1 can be detected non-invasively, it would not only enhance early detection and timely intervention but also reduce the need for invasive procedures, thereby improving animal welfare.

Keywords

Anestrus
Antimicrobial peptides
Biomarker
Cathelicidin-1
Cow

Subjects

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Veterinary Research Forum
Volume 16, Issue 10
October 2025
Pages 573-577

  • Receive Date 14 October 2024
  • Revise Date 14 January 2025
  • Accept Date 01 February 2025

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