Veterinary Research Forum
Vet Res Forum

In vitro physicochemical characterization of nephropathogenic strain of infectious bronchitis virus isolated from poultry

Document Type : Original Article

Authors

Kamran Saaed 1
Faisal Ayub 1
Muhammad Arshad Durrani 1
Abdul Rafay 2
Mobashra Mustafa 1
Irtaqa Gull 1
Zahra Rafi 1
Muhammad Kashan Ansari 1
Muhammad Mubashar Beig 1
Abdul Razak 1
Hafiz Muhammad Moavia Atique 1

1 Institute of Microbiology (IOM), University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

2 Edara Unit Virology, Jaber Al Ahmad Al Sabah Hospital, Kuwait City, Kuwait

https://doi.org/10.30466/vrf.2025.2040770.4445
Abstract
Infectious bronchitis virus (IBV) is an important pathogen in the poultry industry causing avian infectious bronchitis that is, an acute but highly contagious disease affecting the upper respiratory tract, kidneys and reproductive tract. The 3rd passage of a polymerase chain reaction confirmed nephropathogenic IBV isolate was used for this study. Heat stability for 5, 10, 15, 20, and 30 min at 56.00 ˚C, pH sensitivity at pH 3.00, 7.00, 9.00, and 11.00 ultraviolet (UV) irradiation for 10, 15, 20, and 30 min, and chloroform sensitivity were studied. The IBV isolate was found to be susceptible to a temperature of 56.00 ˚C for 5 min and above, UV irradiation within 10 min, chloroform treatment and to pH 11.00 while being resistant to pH 3.00 and 9.00. The second part of the study investigated in vitro effectiveness of the disinfection potential of several commercially used disinfectants in Pakistan against the IBV isolate. For this purpose, Virkon S, Bromosept, and Beloran were employed for the virus inactivation test. Following the IBV challenge for contact time of 1, 5, 10, and 30 min, we counted the number of embryos that died after incubation. Results showed that suitable dilution of disinfectant for the recommended contact period could kill the virus. The maximum susceptibility was seen in the case of Virkon S which killed the virus in just 1 min. Thus, IBV could be killed using commercially available Virkon S, Beloran, and Bromosept after being used in recommended concentrations for recommended contact time.

Keywords

Characterization
Disinfectant
Infectious bronchitis virus
In vitro
Nephropathogenic

Subjects

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Veterinary Research Forum
Volume 16, Issue 12
December 2025
Pages 675-680

  • Receive Date 09 September 2024
  • Revise Date 03 March 2025
  • Accept Date 22 April 2025

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