Veterinary Research Forum
Vet Res Forum

Effects of intraperitoneal administration of Kisspeptin-10 on ischemia-reperfusion injury in a rat testicular torsion and detorsion model

Articles in Press

Document Type : Original Article

Authors

Niki Karvani Dilmaghani 1
Mehdi Behfar 2
Ali Soleimanzadeh 3
Farshid Davoodi 4

1 Veterinary medicine faculty of Urmia University

2 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

4 Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

https://doi.org/10.30466/vrf.2025.2068679.4873
Abstract
Testicular ischemia-reperfusion (I/R) injury during testicular torsion is strongly influenced by oxidative stress caused by excessive accumulation of uncaptured reactive oxygen species (ROS). Kisspeptin-10, a biologically active fragment of the kisspeptin peptide family, has demonstrated significant antioxidant and anti-apoptotic properties. Recent studies indicate that kisspeptin-10 can mitigate oxidative stress by reducing reactive oxygen species levels and enhancing the activity of endogenous antioxidant enzymes such as superoxide dismutase (SOD) and catalase. This study examined the effects of kisspeptin-10 on I/R injury in testicular torsion/detorsion (T/D) of male rats. Twenty male rats were divided into four groups: the control group and three treatment groups (720° T/D, 720° T/D+ 0.50 µg kg-1 kisspeptin-10, 720° T/D+ 1.00 µg kg-1 kisspeptin-10). After inducing 720° clockwise testicular torsion for 2 hr, various factors such as sperm parameters, number, total motility, viability, DNA damage and hypoosmotic test were evaluated. The results showed that 720° T/D can increase sperm DNA damage. In addition, it also had negative effects on overall motility and other properties such as viability and plasma membrane functionality. The results also showed that administration of kisspeptin-10 to T/D rats can reduce DNA damage. These effects could also increase overall motility, viability and plasma membrane functionality compared to the T/D group. Based on our results, kisspeptin-10 provides significant protection against acute T/D injury to the testis when administered after spermatic cord torsion in rat.

Keywords

Ischemia-reperfusion
Kisspeptin-10
Rat
Sperm
Testis

Subjects

Veterinary Research Forum

Articles in Press, Accepted Manuscript
Available Online from 31 December 2025

  • Receive Date 12 August 2025
  • Revise Date 07 October 2025
  • Accept Date 15 November 2025

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