Veterinary Research Forum
Vet Res Forum

Antioxidant, biochemical, hematological indices and handling stress resistance in rainbow trout (Oncorhynchus mykiss) fed with diets supplemented with nano-selenium and vitamin C

Document Type : Original Article

Authors

Ferdows Momeni 1
Saeid Meshkini 2
Ahmad Imani 3

1 PhD Candidate, Department of Fisheries, Faculty of Natural Resources, Urmia University, Urmia, Iran

2 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Fisheries and Aquatic Sciences, Faculty of Natural Resources, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2025.2062525.4786
Abstract
Use of various nanoparticles and vitamins to strengthen the immune system of fish to improve growth and biochemical indice, and to mitigate the harmful effects of free radicals through enhanced antioxidant enzyme activity has become the subject of numerous studies. In this study, fish with an average weight of 55.20 ± 7.90 g, after a two-week acclimation period, were randomly distributed into 18 polyethylene 300-L tanks, with 25 fish per tank. The experiment consisted of six treatments with three replicates: Control, vitamin (Vit) C100, nano-selenium (N-Se)0.40, Vit C100 + N-Se0.10, Vit C200 + N-Se0.20, and Vit C400 + N-Se0.40. Blood samples were collected post-treatment for analysis of antioxidant enzyme activities, biochemical and hematological indices as well as post-handling stress. Results indicated significant differences in antioxidant enzyme activities among treatments. The highest catalase activity was observed in Vit C100 and Vit C100 + N-Se0.10 groups. Glutathione peroxidase activity was significantly higher in the control and Vit C200 + N-Se0.20 groups. Malondialdehyde was the highest in Vit C100 + N-Se0.10 group. Superoxide dismutase activity peaked in Vit C200 + N-Se0.20 group. Biochemical indices showed significant differences among treatments except for high-density lipoprotein. Hemoglobin and red blood cell counts were significantly different. The Vit C100 + N-Se0.10 group showed the highest serum cortisol and glucose levels post-handling stress. Overall, dietary N-Se and Vit C supplementation improved antioxidant, biochemical, hematological indices and stress resistance in rainbow trout.

Keywords

Antioxidant enzyme
Biochemical indices
Nanoparticles
Rainbow trout
 1.     Khan KU, Zuberi A, Nazir S, et al. Synergistic effects of dietary nano selenium and vitamin C on growth, feeding, and physiological parameters of mahseer fish (Tor putitora). Aquac Rep 2017; 5: 70-75.
2.     Aramli MS, Sarvi Moghanlou K, Imani A. Effect of dietary antioxidant supplements (selenium forms, alpha-tocopherol, and coenzyme Q10) on growth performance, immunity, and physiological responses in rainbow trout (Oncorhynchus mykiss) using orthogonal array design. Fish Shellfish Immunol 2023; 134: 108615. doi: 10.1016/j.fsi.2023.108615.
3.     Asadi T, Meshkini S, Ahmadifard N. Dietary effects of procyanidin and Bio-Aqua® on hematological and immune indices of rainbow trout (Oncorhynchus mykiss). Vet Res Forum 2023; 14(7): 359-365.
4.     Harsij M, Gholipour Kanani H, Adineh H. Effects of antioxidant supplementation (nano‑selenium, vitamin C and E) on growth performance, blood biochemistry, immune status and body composition of rainbow trout (Oncorhynchus mykiss) under sub-lethal ammonia exposure. Aquaculture 2020; 521: 734942. doi: 10.1016/j.aquaculture.2020.734942.
5.     Naderi M, Keyvanshokooh S, Salati AP, et al. Effects of dietary vitamin E and selenium nanoparticles supplementation on acute stress responses in rainbow trout (Oncorhynchus mykiss) previously subjected to chronic stress. Aquaculture 2017; 473: 215-222.
6.     Dawood MAO, Koshio S, Zaineldin AI, et al. Dietary supplementation of selenium nanoparticles modulated systemic and mucosal immune status and stress resistance of red sea bream (Pagrus major). Fish Physiol Biochem 2019; 45(1): 219-230.
7.     Wang H, Zhang J, Yu H. Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice. Free Radic Biol Med 2007; 42(10): 1524-1533.
8.     Ashouri S, Keyvanshokooh S, Salati AP, et al. Effects of different levels of dietary selenium nanoparticles on growth performance, muscle composition, blood biochemical profiles and antioxidant status of common carp (Cyprinus carpio). Aquaculture 2015; 446: 25-29.
9.     Saffari S, Keyvanshokooh S, Zakeri M, et al. Effects of different dietary selenium sources (sodium selenite, selenomethionine and nanoselenium) on growth performance, muscle composition, blood enzymes and antioxidant status of common carp (Cyprinus carpio). Aquac Nutr 2016; 23(3): 611-617.
10. Al-Amoudi MM, El-Nakkadi AMN, El-Nouman BM. Evaluation of optimum dietary requirement of vitamin C for the growth of Oreochromis spilurus fingerlings in water from the Red Sea. Aquaculture 1992; 105(2): 165-173.
11. Dabrowski K. Past, present, and future of ascorbic acid research in aquatic organisms. Ascorbic acid in aquatic organisms. 1st ed. Boca Raton, USA: CRC Press 2000; 270-293.
12. Lin YH, Shiau SY. Dietary selenium requirements of juvenile grouper, Epinephelus malabaricus. Aquaculture 2005; 250(1-2): 356-363.
13. Khan KU, Zuberi A, Ullah I, et al. Effects of graded level of dietary l-ascorbyl-2-polyphosphate on growth performance and some hematological indices of juvenile Mahseer (Tor putitora). Int J Agric Biol 2015; 17(4): 821-827.
14. Dawood MAO, Zommara M, Eweedah NM, et al. The potential role of nano-selenium and vitamin C on the performances of Nile tilapia (Oreochromis niloticus). Environ Sci Pollut Res Int 2020; 27(9): 9843-9852.
15. Yarahmadi P, Kolangi Miandare HK, Fayaz S, et al. Increased stocking density causes changes in expression of selected stress- and immune-related genes, humoral innate immune parameters and stress responses of rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 2016: 48: 43-53.
16. Siwicki AK, Anderson DP, Rumsey GL. Dietary intake of immunostimulants by rainbow trout affects non-specific immunity and protection against furunculosis. Vet Immunol Immunopathol 1994; 41(1-2): 125-139.
17. Lewis S, Bain B, Bates I. Dacie and Lewis Practical Hematology. 10th ed. Philadelphia, USA: Churchill Livingstone 2006; 722.
18. Řehulka J. Influence of astaxanthin on growth rate, condition, and some blood indices of rainbow trout, Oncorhynchus mykiss. Aquaculture 2000; 190(1-2): 27-47.
19. Blaxhall P, Daisley KW. Routine haematological methods for use with fish blood. J Fish Biol 1973; 5(6): 771-781.
20. Koller A. Kaplan LA. Total serum protein. In: Kaplan LA, Pesce AJ (Eds). Clinical Chemistry: theory, analysis, and correlation. St. Louis, USA: Mosby 1984;1316-1324.
21. Thomas L. Clinical laboratory diagnostics: use and assessment of clinical laboratory results. Frankfurt, Germany: TH-books Verlagsgesellschaft 1998: 1527.
22. Rifai N, Bachorik PS, Albers JJ. Lipids, lipoproteins and apolipoproteins. Tietz textbook of clinical chemistry. 3rd ed. Florida, USA: WB Saunders 1999: 809-861.
23. Bolann B, Ulvik RJ. Improvement of a direct spectrophotometric assay for routine determination of superoxide dismutase activity. Clin Chem 1991; 37(11): 1993-1999.
24. Góth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991; 196(2-3): 143-151.
25. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Clin Lab Med 1967; 70(1): 158-169.
26. Dawn-Linsley M, Ekinci FJ, Ortiz D, et al. Monitoring thiobarbituric acid-reactive substances (TBARs) as an assay for oxidative damage in neuronal cultures and central nervous system. J Neurosci Methods 2005; 141(2): 219-222.
27. Eslamloo K, Falahatkar B. Variations of some physiological and immunological parameters in Siberian sturgeon (Acipenser baerii, Brandt, 1869) subjected to an acute stressor. J Appl Anim Welf Sci 2014; 17(1): 29-42.
28. Burtis CA, Ashwood ER, Tietz NW. Tietz textbook of clinical chemistry. 3rd ed. Philadelphia, USA: WB Saunders 1999: 29-150.
29. Tietz NW. Clinical guide to laboratory tests (ELISA). In Clinical guide to laboratory tests. 3rd ed. Philadelphia, USA: WB Saunders 1995; 1096-1096.
30. Zhou QC, Mai KS, Tan BP, et al. Partial replacement of fishmeal by soybean meal in diets for juvenile cobia (Rachycentron canadum). Aquac Nutr 2005; 11(3): 175-182.
31. National Research Council. Achieving nutrient and sediment reduction goals in the Chesapeake Bay: an evaluation of program strategies and implementation. Washington DC, USA: National Academies Press 2011.
32. Liang XP, Li Y, Hou YM, et al. Effect of dietary vitamin C on the growth performance, antioxidant ability and innate immunity of juvenile yellow catfish (Pelteobagrus fulvidraco Richardson). Aquac Res 2015; 48(1): 149-160.
33. Abd El-Kader MF, Fath El-Bab AF, Abd-Elghany MF, et al. Selenium nanoparticles act potentially on the growth performance, hemato-biochemical indices, antioxidative, and immune-related genes of European seabass (Dicentrarchus labrax). Biol Trace Elem Res 2021; 199(8): 3126-3134.
34. Khan KU, Zuberi A, Nazir S, et al. Effects of dietary selenium nanoparticles on physiological and biochemical aspects of juvenile Tor putitora. Turk Zool Derg 2016; 40(5): 704-712.
35. Ibrahim MS, El-Gendy GM, Ahmed AI, et al. Nanoselenium versus bulk selenium as a dietary supplement: effects on growth, feed efficiency, intestinal histology, haemato-biochemical and oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus Linnaeus, 1758) fingerlings. Aquac Res 2021; 52(11): 5642-5655.
36. 36.    Le KT, Fotedar R. Immune responses to Vibrio anguillarum in yellowtail kingfish, Seriola lalandi, fed selenium supplementation. J World Aquac Soc 2014; 45(2): 138-148.
37. Dawood MAO, Zommara M, Eweedah NM, et al. Synergistic effects of selenium nanoparticles and vitamin E on growth, immune-related gene expression, and regulation of antioxidant status of Nile tilapia (Oreochromis niloticus). Biol Trace Elem Res 2020; 195(2): 624-635.
38. Hassaan MS, Mohammady EY, Soaudy MR, et al. Exo-genous xylanase improves growth, protein digestibility and digestive enzymes activities in Nile tilapia, Oreochromis niloticus, fed different ratios of fish meal to sunflower meal. Aquac Nutr 2019; 25(2): 841-853.
39. Hassaan MS, Mohammady EY, Adnan AM, et al. Effect of dietary protease at different levels of malic acid on growth, digestive enzymes and haemato-immuno-logical responses of Nile tilapia, fed fish meal free diets. Aquaculture 2020; 522: 735124. doi: 10.1016/j.aquaculture.2020.735124.
40. Bunglavan S, Garg AK, Dass RS, et al. Effect of supplementation of different levels of selenium as nanoparticles/sodium selenite on blood biochemical profile and humoral immunity in male Wistar rats. Vet World 2014; 7(12): 1075-1081.
41. Hao X, Ling Q, Hong F. Effects of dietary selenium on the pathological changes and oxidative stress in loach (Paramisgurnus dabryanus). Fish Physiol Biochem 2014; 40(5): 1313-1323.
42. Abdel-Tawwab M, Mousa MAA, Abbass FE. Growth performance and physiological response of African catfish, Clarias gariepinus (B.) fed organic selenium prior to the exposure to environmental copper toxicity. Aquaculture 2007; 272(1-4): 335-345.
43. Hunt AO, Berkoz M, Ozkan F, et al. Effects of organic selenium on growth, muscle composition, and antioxidant system in rainbow trout. Isr J Aquac 2011; 562(1): 1-10.
44. Yao J, Wang JY, Liu L, et al. Anti-oxidant effects of resveratrol on mice with DSS-induced ulcerative colitis. Arch Med Res 2010; 41(4): 288-294.
45. Naderi M, Keyvanshokooh S, Ghaedi A, et al. Interactive effects of dietary nano selenium and vitamin E on growth, hematology, innate immune responses, antioxidant status, and muscle composition of rainbow trout under high rearing density. Aquac Nutr 2019; 25(5): 1156-1168.
46. Affonso EG, Silva Eda C, Tavares-Dias M, et al. Effect of high levels of dietary vitamin C on the blood responses of matrinxã (Brycon amazonicus). Comp Biochem Physiol A Mol Integr Physiol 2007; 147(2): 383-388.
47. Abdel-Daim MM, Abushouk AI, Donia T, et al. The nephroprotective effects of allicin and ascorbic acid against cisplatin-induced toxicity in rats. Environ Sci Pollut Res Int 2019; 26(13): 13502-13509.
48. Merrifield D, Bradley G, Baker RTM, et al. Probiotic applications for rainbow trout (Oncorhynchus mykiss Walbaum) II. effects on growth performance, feed utilization, intestinal microbiota and related health criteria postantibiotic treatment. Aquac Nutr 2010; 16(5): 496-503.
Veterinary Research Forum
Volume 17, Issue 1
January 2026
Pages 39-45

  • Receive Date 07 February 2026

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