Veterinary Research Forum
Vet Res Forum

Molecular investigation of biofilm-forming genes in staphylococci isolated from dogs in Ilam, Iran

Document Type : Original Article

Authors

Negar Soltani
Mostafa Nemati
Fazel Pourahmad

Department of Microbiology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran

10.30466/vrf.2025.2052777.4656
Abstract
Biofilm formation is a key virulence factor in Staphylococcus aureus, contributing to bacterial persistence, antimicrobial resistance, and chronic infections. This study aimed to investigate the presence of biofilm-associated genes (fib, fnbA, fnbB, clfA, and clfB) in S. aureus isolates from dogs in Ilam, Iran. From December 2022 to September 2023, 250 swab samples were collected from nasal, oral, and rectal sites of dogs, yielding 81 S. aureus isolates confirmed by PCR amplification of the nuc gene. The prevalence of biofilm-associated genes varied, with clfA, clfB, and fnbA detected in 98.80% of isolates, fib in 63.00%, and fnbB in 16.00%. Notably, fnbA, clfA, and clfB were present in all rectal isolates, while fnbB was absent in this group. The findings highlighted the widespread presence of biofilm-related genes in S. aureus from dogs, suggesting their potential role in colonization and zoonotic transmission. The high prevalence of adhesion-associated genes underscored the need for monitoring biofilm-forming S. aureus in companion animals to mitigate antimicrobial resistance and public health risks.

Keywords

Biofilm
Dogs
Staphylococcus aureus
Virulence factors
Zoonoses

Subjects

Veterinary Research Forum
Volume 17, Issue 3
March 2026
Pages 191-197

  • Receive Date 06 February 2025
  • Revise Date 23 April 2025
  • Accept Date 20 May 2025

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