Microbiology
Masoud Amini; Abdulghaffar Ownagh; amir Tokmachi; Manochehr Allymehr
Articles in Press, Accepted Manuscript, Available Online from 02 December 2023
Abstract
The poultry products are known as a resource of zoonotic and multi drug resistant pathogens, especially Salmonella spp. Using bacteriophages as an alternative antimicrobial agent are objective. Salmonella typhimurium isolate from turkey poults. The antibiotic susceptibility test was used identified the ...
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The poultry products are known as a resource of zoonotic and multi drug resistant pathogens, especially Salmonella spp. Using bacteriophages as an alternative antimicrobial agent are objective. Salmonella typhimurium isolate from turkey poults. The antibiotic susceptibility test was used identified the antibiotic resistance pattern of the isolates. The bacteriophage was purified, enhanced, and titrated using the Spot test and Double Layer Agar techniques after being isolated from a chicken slaughterhouse and sewage treatment facility. By determining the morphological characteristics of resulting plaque, the specificity and host range of the phage were studied on S. typhimurium isolates. The number of 22 suspected Salmonella isolates was confirmed biochemically positive in sample by cultures method. Nine of these isolates (40.90%) were identified as Salmonella typhimurium by Polymerase Chain Reaction. All of isolates (100%) were resistant to Chloramphenicol, Doxycycline, Kanamycin, Florfenicol, Rifampin, and Erythromycin. Seven isolates (77.70%) were resistant to Amoxicillin and Nalidixic acid. The plaques were present with a diameter of 3 ± 0.22 mm on the culture of 6 out of 9 (66.60%) isolates of S. typhimurium on the B.H.I broth medium using Double Layer Agar method. The amount of phage titer was 7.6 × 107 pfu/ mL and its Multiplicity of infection value was calculated as 5.06 × 10-2 based on obtained results. In place of antibiotics, the MDR S. typhimurium was successfully destroyed by the isolated bacteriophage from wastewater. In vitro settings were used in this investigation to identify the efficient bacteriophages against MDR S. typhimurium.
Mohadeseh Barazandeh; Khashayar Shahin; Abolghasem Hedayatkhah; Majid Komijani; Mojtaba Mansoorianfar
Volume 12, Issue 4 , December 2021, , Pages 401-407
Abstract
Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of ...
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Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.
