Veterinary Research Forum
Vet Res Forum

Effect of treating recycled poultry bedding with tannin extracted from pomegranate peel on rumen fermentation parameters and cellulolytic bacterial population in Arabian fattening lambs

Document Type : Original Article

Authors

Afrooz Sharifi 1
Morteza Chaji 1
Alirez Vakili 2

1 Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran

2 Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

https://doi.org/10.30466/vrf.2019.75050.2007
Abstract
This study was conducted to investigate the effects of recycled poultry bedding (RPB) treated with different levels of pomegranate peel extract (PPE) as a tannin source on cellulolytic bacterial population and rumen fermentation parameters of fattening lambs. For this purpose, twenty-eight Arabian lambs (19.70 ± 2.45 kg body weight, 90 ± 12 days of age) were randomly assigned to four dietary treatments. Recycled poultry bedding was treated with PPE at four levels of 0 (control), 20.00, 25.00 and 30.00% on DM basis. Bacterial populations were enumerated by DNA extraction of samples of rumen liquor followed by real-time polymerase chain reaction analysis. Also, rumen samples were evaluated for pH, volatile fatty acid (VFA) and ammonia nitrogen (AN) concentrations. The populations of total bacteria, Ruminococcusalbus and Fibrobacter succinogenes were decreased significantly as the level of PPE in the diet increased, however, the population of Ruminococcus flavefaciens was not affected. Dietary treatments did not have effect on ruminal pH, while AN concentration was decreased in the diets containing RPB treated with PPE compared to the control. Concentrations of total VFA and individual VFA remained unchanged by PPE-treated RPB inclusion in the diet. In conclusion, supplementing RPB with PPE improved nitrogen metabolism of fattening lambs, however, it decreased population of rumen cellulolytic bacteria R. flavefaciens.

Keywords

Cellulolytic bacteria
Pomegranate peel
Real-time PCR
Recycled poultry bedding

Subjects

  1.  

    1. Daniel J, Olson KC. Feeding poultry litter to beef cattle. MU Guide. MU Extension, University of Missouri-Columbia, 2005: G2077.
    2. Rankins DL, Poore MH, Capucille DJ, et al. Recycled poultry bedding as cattle feed. Vet Clin North Am Food Anim Prac 2002; 18(2): 253-266.
    3. Azizi-Shotorkhoft A, Rouzbehan Y, Fazaeli H. The influence of the different carbohydrate sources on utilization efficiency of processed broiler litter in sheep. Livest Sci 2012; 148(3): 249-254.
    4. Zinn RA, Barajas R, Montan M, et al. Protein and energy value of dehydrated poultry excreta in diets for feedlot cattle. J Anim Sci 1996; 74(10): 2331-2335.
    5. Jolazadeh AR, Dehghan-banadaky M, Rezayazdi K. Effects of soybean meal treated with tannins extracted from pistachio hulls on performance, ruminal fermentation, blood metabolites and nutrient digestion of Holstein bulls. Anim Feed Sci Technol 2015; 203: 33-40.
    6. Abarghuei MJ, Rouzbehan Y, Salem AZM, et al. Nutrient digestion, ruminal fermentation and performance of dairy cows fed pomegranate peel extract. Livest Sci 2013; 157(2-3): 452-461.
    7. El-Waziry AM, Nasser ME, Sallam SMA. Processing methods of soybean meal. 1- Effect of roasting and tannic acid treated soybean meal on gas production and rumen fermentation in vitro. J Appl Sci Res 2005; 1(3): 313-320.
    8. Makkar HPS. Effects and fate tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Rumin Res 2003; 49(3): 241-256.
    9. Hagerman AE and Butler LG. The specificity of proanthocyanidin- protein interactions. J Biol Chem 1981; 256(9): 4494-4497.
    10. McSweeney CS, Palmer B, Bunch R, et al. Effect of the tropical forage Calliandra on microbial protein synthesis and ecology in the rumen. J Appl Microbiol 2001; 90(1): 78-88.
    11. Min B, Barry T, Attwood G, et al. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: a review. Anim Feed Sci Technol 2003; 106(1-4): 3-19.
    12. Barry TN, Manley TR, Duncan SJ. The role of condensed tannins in the nutritional value of Lotus pedunculatus for sheep. 4. Sites of carbohydrate and protein digestion as influenced by dietary reactive tannin concentration. Br J Nutr 1986; 55(1): 123-137.
    13. Adamczyk B, Simon J, Kitunen V, et al. Tannins and their complex interaction with different organic nitrogen compounds and enzymes. Chem Open 2017; 6(5): 610-614.
    14. Nagpal R, Puniya AK, Griffith GW, et al. Anaerobic rumen fungi: Potential and applications. In: Khachatourians GG, Arora DK, Rajendran TP, et al. (Eds). Agriculturally important micro-organisms. Kamal, India: Academic World International 2009: 375-393.
    15. Patra Ak. Dietary tannins on microbial ecology of the gastrointestinal tract in ruminants. In: Patra Ak (Eds). Dietary phytochemicals and microbes. Dordrecht, Netherlands: Springer Science & Business Medi: 2012; 237-256.
    16. Akin DE, Rigsby LL, Theodorou MK, et al. Population changes of fibrolytic rumen bacteria in the presence of phenolic acids and plant extracts. Anim Feed Sci Technol 1988; 19(3): 261-275.
    17. Ghasemi S, Naserian AA, Valizadeh R, et al. Inclusion of pistachio hulls as a replacement for alfalfa hay in the diet of sheep causes a shift in the rumen cellulolytic bacterial population. Small Rumin Res 2012; 104(1-3): 94-98.
    18. Bhatta R, Uyeno Y, Tajima K, et al. Difference in the nature of tannins on in vitro ruminal methane and volatile fatty acid production and on methanogenic archaea and protozoal populations. J Dairy Sci 2009; 92(11): 5512-5522.
    19. Wanapat M, Cherdthong A. Use of real-time PCR technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo. Curr Microbiol 2009; 58(4): 294-299.
    20. Capparucci C, Gironi F, Piemonte V. Tannins extraction from walnuts residues. Chem Eng Trans 2011; 24: 469-474.
    21. Sharifi A, Chaji M, Vakili A. Effect of treatment recycled poultry bedding with pomegranate peel tannins extract on in vitro digestion and fermentation activity of cow rumen microorganisms [Persian]. J Anim Prod 2017; 19(2):321-336.
    22. International Atomic Energy Agency website (IAEA). Quantification of Tannins in Tree Foliage. Available at: http://www.naweb.iaea.org/nafa/aph/public/pubd31022manual-tannin.pdf. Accessed Nov 11, 2017.
    23. National Research Council. National research council nutrient requirements of small ruminants, sheep, goats, Cervids and new world Camelids. Washington, USA: National Academy of Science 2007; 244-270.
    24. Jasmin BH, Boston RC, Modesto RB, et al. Perioperative ruminal pH changes in domestic sheep (Ovis aries) housed in a biomedical research setting. J Am Assoc Lab Anim Sci 2011; 50(1): 27-32.
    25. AOAC. Official methods of analysis of the association of official analytical chemists. 15th ed. Arlington, USA: Association of Official Analytical Chemists 1990; 69-88.
    26. Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74(10): 3583-3597.
    27. Robertson JB, Van Soest PJ. The detergent system of analysis and its application to human foods. In: Sames WPT, Theander O (Eds). The analysis of dietary fibre in food. New York, USA: Marcel Dekker 1981; 123-158.
    28. Broderick G A, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. J Dairy Sci 1980; 63(1): 64-75.
    29. Stewart CS, Duncan SH. The effect of avoparcin on cellulolytic bacteria of the ovine rumen. J Gen Microbiol 1985; 131: 427-435.
    30. Valizadeh R, Behgar M, Mirzaee M, et al. The effect of physically effective fiber and soy hull on the ruminal cellulolytic bacteria population and milk production of dairy cows. Asian-Aust J Anim Sci 2010; 23(10): 1325-1332.
    31. Stewart CS, Flint HJ, Bryant MP. The rumen bacteria. In: Hobson PN, Stewart CS (Eds). The rumen microbial ecology. Dordrecht, Netherlands: Springer 1997; 10-54.
    32. Ghasemi S, Naserian AA, Valizadeh R, et al. Partial and total substitution of alfalfa hay by pistachio byproduct modulated the counts of selected cellulolytic ruminal bacteria attached to alfalfa hay in sheep. Livest Sci 2012, 150(1-3): 342-348.
    33. Makkar HPS, Blummel M, Becker K. In vitro effects of and interactions between tannins and saponins and fate of tannins in the rumen. J Sci Food Agric 1995; 69: 481-493.
    34. Goel G, Puniya AK, Singh K. Xylanolytic activity of ruminal Streptococcus bovis in presence of tannin acid. Annu Microbiol 2005; 55(4): 295-297.
    35. Bae HD, McAllister TA, Yanke J, et al. Effects of condensed tannins on endoglucanase activity and filter paper digestion by Fibrobacter succinogenes S85. Appl Environ Microbiol 1993; 59(7): 2132-2138.
    36. Molan AL, Attwood GT, Min BR, et al. The effect of condensed tannins from lotus pedunculatus and lotus corniculatus on the growth of proteolytic rumen bacteria in vitro and their possible mode of action. Can J Microbiol 2001; 47(7): 626-633.
    37. Newbold CJ, Elhassan SM, Wang J, et al. Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria. B J Nutr 1997; 78(2): 237-249.
    38. Bento MHL, Acamovic T, Makkar HPS. The influence of tannin, pectin and polyethylene glycol on attachment of 15N-labelled rumen microorganisms to cellulose. Anim Feed Sci Technol 2005; 122(1-2): 41-57.
    39. McAllister TA, Bae HD, Jones GA, et al. Microbial attachment and feed digestion in the rumen. J Anim Sci 1994; 72(11): 3004-3018.
    40. Min BR, Attwood GT, McNabb WC et al. The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and growth of rumen bacteria. Anim Feed Sci Technol 2005; 121(1-2): 45-58.
    41. Cheng K J, Costerton JW. Microbial adhesion and colonization within the digestion tract. Soc Appl Bacteriol Symp Ser 1986;13: 239-261.
    42. Singh B, Chaudhary LC, Agarwal N, et al. Effect of feeding Ficus infectoria leaves on rumen microbial profile and nutrient utilization in goats. Asian-Aust. J Anim Sci 2011; 24 (6): 810-817.
    43. Van Soest PJ. Nutritional ecology of the ruminant. Ithaca, USA: Cornell University Press 1994; 230-280.
    44. Yildiz S, Kaya I, Unal Y, et al. Digestion and body weight change in Tuj lambs receiving oak (Quercus hartwissiana) leaves with and without PEG. Anim Feed Sci Technol 2005; 122(1-2): 159-172.
    45. Min BR, Pinchak WE, Fulford JD, et al. Wheat pasture bloat dynamics, in vitro ruminal gas production, and potential bloat mitigation with condensed tannin. J Anim Sci 2005; 83(6): 1322-1331.
    46. Krueger WK, Gutierrez-Banuelos H, Carstens GE, et al. Effects of dietary tannin source on performance, feed efficiency, ruminal fermentation, and carcass and non-carcass traits in steers fed a high-grain diet. Anim Feed Sci Technol 2010; 159(1-2): 1-9.
    47. Theodoridou K, Aufrere J, Niderkorn V, et al. In vitro study of the effects of condensed tannins in sanfoins on the digestive process in the rumen at two vegetation cycles. Anim Feed Sci Technol 2011; 170(3-4): 147-159.
    48. Tan H, Sieo C, Abdullah N, et al. Effects of condensed tannins from Leucaenaon methane production, rumen fermentation and populations of methanogens and protozoa in vitro. Anim Feed Sci Technol 2011; 169(3-4): 185-193.
Veterinary Research Forum
Volume 10, Issue 2
Spring 2019
Pages 145-152

  • Receive Date 11 November 2017
  • Revise Date 25 December 2017
  • Accept Date 09 January 2018

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