The challenge of getting a high quality of RNA from oocyte for gene expression study

Document Type: Short Communication

Authors

1 Institute of Advanced Studies, University of Malaya, Kuala Lumpur, Malaysia

2 Biology Division, Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, Malaysia

3 Centre for Research in Biotechnology for Agriculture, University of Malaya, Kuala Lumpur, Malaysia

Abstract

The extraction of intact RNA from oocyte is quite challenging and time-consuming. A standard protocol using commercial RNA extraction kit, yields a low quantity of RNA in oocytes. In the past, several attempts in getting RNA for gene expression study ended up with a few different modified methods. Extraction of high-quality RNA from oocyte is important before further downstream analyses such as reverse transcription-polymerase chain reaction, quantitative polymerase chain reaction, or northern blot analysis. In this review, the efficiency of RNA extraction methods from all species oocytes was compared between published articles and our research to gather all possible methods of RNA extraction. Two different methods of RNA extraction that were proposed from various experiments were reviewed to determine the best method of RNA extraction from the oocyte. Modified TRIzol method can be concluded as an efficient RNA extraction method especially for good RNA from oocytes. Meanwhile, comparing RNA extraction kits to extract the RNA from oocytes or pre-implantation embryos, the micro RNA extraction kit type is the best. Therefore, an appropriate RNA extraction method is important to obtain high quality of total RNA for gene expression profiling analysis.

Keywords