Sara Dehyadegari; Mohammad Mehdi Oloumi; Shahrzad Azizi
Volume 11, Issue 1 , March 2020, , Pages 15-20
Abstract
This study was performed to evaluate the role of electroacupuncture on kidney 1 (Ki1) acupoint to prevent the heart and brain injury following ischemia/reperfusion of both kidneys. 24 Sprague Dawley rats were randomly assigned into four equal groups. In the treatment 1 group, following anesthesia, acupuncture ...
Read More
This study was performed to evaluate the role of electroacupuncture on kidney 1 (Ki1) acupoint to prevent the heart and brain injury following ischemia/reperfusion of both kidneys. 24 Sprague Dawley rats were randomly assigned into four equal groups. In the treatment 1 group, following anesthesia, acupuncture needles were inserted on Ki1 on the palm of both hindlimbs and connected to electroacupuncture unit for a 3.00 Hz direct current, 1 hr before surgery until the end of surgery. In treatment two groups, the electroacupuncture was also performed 48 and 24 hr before the operation, with the same protocol as treatment 1. Control 1 and control 2 groups had the same procedures like the treatment ones, except for acupuncture. Immediately after reperfusion, the samples of brains and hearts were taken and prepared for microscopic examination. Histopathological study of the heart in the control and treatment groups showed the breakage of myofibrils, hyaline necrosis, edema and disorganization of myocytes. The severity of cardiac lesions was decreased in both treatment groups in comparison with the controls. Brain in control and treatment groups showed ischemic necrosis, disorganization of the neurons in the hippocampus, and edema. The severity of lesions was reduced in the treatment groups and showed a significant difference between the control and treatment 1. It could be concluded that electroacupuncture on the Ki1 point could reduce the severity of damages induced by renal ischemia/reperfusion in the remote organs of the heart and brain.
Surgery
Amin Paidar Ardakani; Mohammd Mehdi Oloumi; Alireza Farsinejad; Reza Kheirandish
Volume 10, Issue 4 , December 2019, , Pages 285-291
Abstract
The present study was designed to evaluate the effects of platelet growth factors and periosteal mesenchymal stem cells on bone healing process, radiographically. Forty male White New Zealand rabbits in five equal groups were used in this study. A 2 mm full thickness bone defect was made in left radial ...
Read More
The present study was designed to evaluate the effects of platelet growth factors and periosteal mesenchymal stem cells on bone healing process, radiographically. Forty male White New Zealand rabbits in five equal groups were used in this study. A 2 mm full thickness bone defect was made in left radial bone of each animal. In group A (control) the defect was left with no medical intervention. In group B the defect was covered by a fibrin membrane. In group C the defect was covered by a fibrin membrane plus platelet growth factors. In group D the defect was covered by a fibrin membrane plus periosteal mesenchymalstem cells, and in group E the defect was covered by a fibrin membrane enriched with platelet growth factors and periosteal mesenchymalstem cells. Radiological evaluation was done immediately after surgery (week 0) and then at the 1st, 2nd, 4th, 6th and 8th weeks after operation. At the end of the eighth week, bone samples were taken to evaluate the histopathology. The radiological and histopathological observations showed a superior bone healing in the groups D and E, after eight weeks in comparison with the groups A, B and C. According to this study, it could be concluded that the platelet growth factors and periosteal mesenchymalstem cells could promote bone regeneration in long bone defects in a rabbit model.
