Large Animal Internal Medicine
Yousef Davoudi; Mohammad nouri; Mohammad Rahim Haji Hajikolaie; Shobeir Yazdani Paraei; Amir Javadi; Saleh Esmaeilzadeh
Articles in Press, Accepted Manuscript, Available Online from 02 May 2024
Abstract
In November 2021, a cattle farm located in Dasht-e-Moghan city of Ardabil province experienced a concerning incident. During the visit to the farm, 70 cows experienced stillbirths, abortions, and the births of calves with congenital abnormalities such as arthrogryposis and hydranencephaly To investigate ...
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In November 2021, a cattle farm located in Dasht-e-Moghan city of Ardabil province experienced a concerning incident. During the visit to the farm, 70 cows experienced stillbirths, abortions, and the births of calves with congenital abnormalities such as arthrogryposis and hydranencephaly To investigate the cause of this outbreak, brain tissue samples of two calves with hydranencephaly that died within days after their birth were analyzed. PCR testing was conducted for multiple viruses, including Bovine viral diarrhea (BVD), Border disease, Akabane, Schmallenberg, and Bluetongue viruses. The results indicated positive only for Akabane virus. Additionally, blood samples were collected from 60 cows and calves that were over 8 months old to determine the presence of antibodies against the Akabane and Schmallenberg viruses. Blood samples were collected from 60 cows and calves over 8 months of age. Due to limited facilities, only antibodies against Akaban and Schmallenberg viruses were examined using the Competitive Enzyme Linked Immunosorbent Assay (C-ELISA). Out of the 60 cows and calves tested, 12 cows were found to have antibodies against the Akaban virus, and 15 cows had antibodies against the Schmallenberg virus. Interestingly, nine cows tested positive for both the Akaban and Schmallenberg viruses.In conclusion, based on clinical findings and results, it appears that the diagnosed disease is Akaban. However, it is important to note that further investigation is needed to determine the source of the virus and their transmission route.
Masoud Reza Seyfi Abad Shapouri; Pezhman Mahmoodi; Masoud Ghorbanpour Najafabadi; Mohammad Rahim Haji Hajikolaei; Parastoo Moradi Choghakabodi; Mohsen Lotfi; Mahdi Pourmahdi Boroujeni; Maryam Ekhtelat; Maryam Daghari
Volume 13, Issue 3 , September 2022, , Pages 403-407
Abstract
Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS3), as a single ELISA recombinant ...
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Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS3), as a single ELISA recombinant antigen, along with a monoclonal antibody to detect antibodies against BVDV in sera of infected animals. Hence, 197 serum samples were tested by this cELISA and the results were compared to the results obtained from virus neutralization test (VNT) as the gold standard method for diagnosis of BVD. McNemar’s test indicated that there was no significant difference between the results of this newly designed cELISA and VNT. Meanwhile, kappa coefficients showed that there was a high correlation between these two assays. The relative sensitivity and specificity of cELISA with respect to VNT were 93.90% and 100%, respectively, suggesting that this newly designed cELISA could be a useful diagnostic tool for detection of BVDV infection. Moreover, as NS3 is highly conserved among Pestiviruses and the developed ELISA is a competitive one, it could potentially be applied to detect BVDV infection in other domestic and wildlife species.
Mohammad Khosravi; Mohammad Rahim Haji Hajikolaie; Shahrzad Alipour; Ara Ameri; Mohammad Bafandeh Dehaghi
Volume 11, Issue 3 , September 2020, , Pages 285-288
Abstract
The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) ...
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The penicillin allergy is being increasingly recognized as a significant public health problem. Immunological responses to penicillin and other beta-lactam antibiotics are classified as immediate and non-immediate responses. This research aimed to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of the reactive antibody value against penicillin in various species of animals. The serum samples were collected from nine species (forty mature animals in each species) including horse, dog, goat, sheep, buffalo, cattle, donkey, chicken, and fish. The concentrations of total antibody and immunoglobulin M (IgM) against penicillin were detected using an in-house ELISA test. The total anti-penicillin antibodies concentration from high to low in animals was as chicken, horse, fish, donkey, dog, goat, sheep, buffalo, and cattle, respectively. In cattle and sheepthe level of anti-penicillin IgM (APM) was significantly higher than non-IgM antibodies (APNM); moreover, levels of APNM were very low in chicken and fish serums; no difference was seen regarding these values in buffalo and goat. The other species had significantly lower APM than the APNM. The ani-penicillin antibody levels in the noted animals were successfully detected using the developed ELISA. Most of the species have anti-penicillin antibodies; however, they have reactive antibodies with differences in levels and isotypes.
