Filiz Kazak; Gul Fatma Yarim; Ayris Gokceoglu; Mehmet Kemal Delmecioglu; Murat Yarim
Volume 13, Issue 4 , December 2022, , Pages 593-596
Abstract
Epidermal growth factor (EGF) has biological roles, including embryonic organ development, breast morphogenesis, breast cell proliferation, and mammary development. This study aimed to measure EGF concentration and evaluate its relationship with somatic cell count (SCC) in healthy water buffaloes (Bubalus ...
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Epidermal growth factor (EGF) has biological roles, including embryonic organ development, breast morphogenesis, breast cell proliferation, and mammary development. This study aimed to measure EGF concentration and evaluate its relationship with somatic cell count (SCC) in healthy water buffaloes (Bubalus bubalis) milk. The study material was constituted of 120 milk samples obtained from 30 healthy water buffaloes between the ages of 3 - 6 years, negative for California mastitis test and SCC less than 3.00 × 105 cells mL-1 milk. In milk serum samples, the EGF concentration was measured using a bovine-specific enzyme-linked immunosorbent assay kit. Epidermal growth factor concentration in the buffalo milk was ranged from 4.30 to 9.80 ng mL-1, with a mean of 8.30 ± 1.50 ng mL-1. Positive correlation between milk SCC values and EGF concentrations was recorded in water buffaloes. Further research is required to evaluate the content of milk EGF in different species of animals because of the EGF effective role in mammary gland and intestinal mucosa.
Sina Moghaddam; Amir Farhang Houshangi; Behrad Eshratkhah; Rahman Allahvirdizadeh
Volume 12, Issue 1 , March 2021, , Pages 133-135
Abstract
Congenital ichthyosis is a disease associated with hair loss and the presence of horny plates in the epidermis, covering the whole skin. The leading cause of the disease in humans and animals is genetic disorders, and they can be found in mild and severe forms. In June 2015, a newly born calf of the ...
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Congenital ichthyosis is a disease associated with hair loss and the presence of horny plates in the epidermis, covering the whole skin. The leading cause of the disease in humans and animals is genetic disorders, and they can be found in mild and severe forms. In June 2015, a newly born calf of the Holstein breed was referred to the Livestock Veterinary Hospital internal ward, Shabestar, Iran. The calf's clinical symptoms included maternal alopecia, thickening, and fissure over large areas of the body, tough skin with thick horny scales and deep crack, lack of flexibility in the body parts (gluteal, knee, and shoulder areas), ectropion, eclabium, and microtia. The blood samples were taken from the calf's jugular vein to measure the hematological and biochemical parameters. After euthanizing the calf, the skin of different body regions was sampled for histopathological examination of skin lesions. Based on the results, the amounts of plasma parameters such as urea, triglyceride, glucose, alanine transaminase, lactate dehydrogenase, phosphorus, and uric acid were increased. The leukocytosis and polycythemia were found in the hematology results, and histopathological analysis exhibited hypergranulosis and hyperkeratosis in the skin of affected areas. Ichthyosis is caused by the defect in the autosomal recessive gene and as an incurable disease; there is currently no cure for this deadly disease, and the livestock will be eliminated from the herd.
Behrokh Marzban Abbas Abadi; Hannaneh Golshahi; Saeed Seifi
Volume 12, Issue 2 , June 2021, , Pages 263-265
Abstract
Spondylolisthesis known as kinky back syndrome is a deformity of the free sixth thoracic vertebra including enlargement and rotation resulting in posterior paralysis in broiler chicken. Genotype, feeding regimes and environment are involved in occurrence of this congenital anomaly. Spondylolisthesis ...
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Spondylolisthesis known as kinky back syndrome is a deformity of the free sixth thoracic vertebra including enlargement and rotation resulting in posterior paralysis in broiler chicken. Genotype, feeding regimes and environment are involved in occurrence of this congenital anomaly. Spondylolisthesis may result in or happen simultaneously with two other major axial skeleton pathologies including spondylosis and spondylitis as a result of occurrence of degenerative necrobiotic lesions and inflammation of the vertebrae, respectively. This report deals with a male broiler chicken with posterior paralysis in the second week of rearing. After euthanasia, the specimen was evaluated at macroscopic and microscopic levels. Macroscopic findings showed the unilateral enlargement of body and transverse process of sixth thoracic vertebra and downward rotation of the body. The histopathological studies revealed the focal chondronecrosis and degenerative changes in articular facets and cranial process of the vertebra. In conclusion, a diagnosis of spondylolisthesis followed by spondylosis was made based on macroscopic and histopathological findings that to the authors’ best knowledge, is the first report in a broiler farm in Iran.
Farshid Davoodi; Masoud Selk Ghaffari; Abbas Raisi; Omid Dezfoulian
Volume 12, Issue 3 , September 2021, , Pages 391-393
Abstract
Ocular tumors are rarely seen in farm animals. Iridociliary epithelial tumors are the second most common tumor of the eye in dogs and cats, but there is just one report of this tumor in sheep. This case report described the history, clinical signs, histopathological and immunohistochemical findings, ...
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Ocular tumors are rarely seen in farm animals. Iridociliary epithelial tumors are the second most common tumor of the eye in dogs and cats, but there is just one report of this tumor in sheep. This case report described the history, clinical signs, histopathological and immunohistochemical findings, and surgical management of an iridociliary adenoma tumor in a 3-year-old Iranian Shaal sheep with signs of anorexia, depression, loss of vision, and a mass covering the left eye. The mass had protruded from the left eye globe but had not penetrated the periocular tissues. Enucleation was performed to excise the tumor. Periodic acid–Schiff (PAS) and Alcian blue staining methods were done for the mass, but the result for both of them was negative. Slides were also immunostained for antibodies against cytokeratin AE1/AE3, vimentin, and S-100 protein. The results were positive for vimentin but negative for cytokeratin and S-100. According to the results of histopathology and immunohistochemistry, the type of tumor was diagnosed as iridociliary adenoma. To our knowledge, it was the second report of the iridociliary tumor in the sheep and the first report of this tumor in the Iranian Shaal breed.
Mostafa Meshkat; Bahar Shemshadi; Kumarss Amini
Volume 13, Issue 4 , December 2022, , Pages 597-601
Abstract
The present study was conducted to investigate the detection and identification of Cryptosporidium species via molecular techniques and evaluate the serum concentrations of inflammatory factors in Cryptosporidium species. The fecal samples (n = 256) were collected from pre-weaned (≤ 2.00 months) calves ...
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The present study was conducted to investigate the detection and identification of Cryptosporidium species via molecular techniques and evaluate the serum concentrations of inflammatory factors in Cryptosporidium species. The fecal samples (n = 256) were collected from pre-weaned (≤ 2.00 months) calves and the positive samples were identified utilizing Ziehl-Neelsen staining. Nested species-specific multiplex PCR (nssm-PCR) and restriction fragment length polymorphism (RFLP) were used to identify the species and sub-species. The serum concentrations of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were also assessed. The results revealed that 10.54% of samples were positive. The results of Nested-PCR showed that 92.59% of the samples were positive for C. parvum while 7.41% were positive for C. andersoni. The results of RFLP confirmed 92.59% of the samples for C. parvum, 3.70% for C. muris / C. andersoni, and 3.70% for C. muris. The serum concentrations of IL-1β, IL-6, IL-12, TNF-α, and IFN-γ were significantly higher in the infected calves compared to those in healthy calves. However, the serum concentration of IFN-γ was significantly higher in the calves infected with C. parvum while the serum concentrations of TNF-α and IL-6 were significantly higher in those infected with C. andersoni. In conclusion, C. parvum was prevalent in the region and the calves demonstrated inflammatory responses to Cryptosporidium species.
Azadeh Yektaseresht; Zahra Hemati; Amir Arsalan Khorsand; Shoor Virsingh
Volume 13, Issue 4 , December 2022, , Pages 603-606
Abstract
No diagnostic kits and reagents are available in the market to detect and evaluate camel immune responses to different pathogens. This study aimed to produce sheep anti-camel (Camelus dromedarius) polyclonal antibodies (pAbs) and to determine the specificity with other species immunoglobulin. Immunoglobulins ...
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No diagnostic kits and reagents are available in the market to detect and evaluate camel immune responses to different pathogens. This study aimed to produce sheep anti-camel (Camelus dromedarius) polyclonal antibodies (pAbs) and to determine the specificity with other species immunoglobulin. Immunoglobulins (Igs) from camel serum samples were purified using ammonium sulfate precipitation (40.00% saturated ammonium sulfate). Purity of the camel Igs was tested by sodium dodecyl sulfate polyacrylamide gel electrophoresis. PAbs against (Camelus dromedarius) immunoglobulins were generated by immunizing sheep with purified Igs. Anti- camel Ig polyclonal antibodies titer and specificity were determined using ELISA and Western blot techniques. Polyclonal antibodies specific to camel Igs were significantly high in immunized sheep which confirmed the immunization procedure. PAbs reacted specifically with camel serum immunoglobulin and did not react with other species immunoglobulin of horse and chickens. Polyclonal antibodies produced in this study can be regarded as a valuable tool to be used for immune-diagnostic purposes in camel population world-wide.
Hossein Kazemi Mehrgerdi; Masoud Rajabioun; Ali Mirshahi; Ensiyeh Sajjadian Jaghargh
Volume 13, Issue 4 , December 2022, , Pages 607-610
Abstract
Diaphragmatic rupture is introduced as one of the most common injuries occurring in small animals. In our retrospective report, 14 cats with a mean age of 12 months (male = 8, female = 6) that underwent diaphragmatic herniorrhaphy were studied. The data included the following: age, sex, breed, and clinical ...
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Diaphragmatic rupture is introduced as one of the most common injuries occurring in small animals. In our retrospective report, 14 cats with a mean age of 12 months (male = 8, female = 6) that underwent diaphragmatic herniorrhaphy were studied. The data included the following: age, sex, breed, and clinical signs. The cause of diaphragmatic hernia, diaphragmatic rupture area, herniated organs, concomitant injuries, and survival rate after surgical treatment were detailed. The cats included 11 domestic shorthair (DSH) and three Persian. Trauma was the most common cause of rupture in these patients and one 3-month old Persian cat had peritoneopericardial diaphragmatic hernia (PPDH). The most common clinical sign was dyspnea. Concurrent disorders included fractures of pelvic, long bones and ribs and mild pneumothorax. One of the five cats survived with concurrent disorders. In this study, the frequency of rupture sites was as follows: the right-side (11 cats), left-side (2 cats), and dorsal side of diaphragm in one case. The most frequently inserted organ into the chest was liver. In our study, the survival rate after surgical treatment was 71.00%. In conclusion, we suggested that stabilization of the patient was important before herniorrhaphy. Based on our study, the concurrent damages affected the survival rate.
Saeed Khatemeh; Abbas Imani Baran
Volume 13, Issue 4 , December 2022, , Pages 611-614
Abstract
Different living organisms are used as applicable bioindicators to determine heavy metal pollutions. Recent studies have shown that helminths parasites can be used as efficient environmental sentinels. This study aimed to evaluate Fasciola hepatica and Dicrocoelium dendriticum as bioaccumulators of lead ...
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Different living organisms are used as applicable bioindicators to determine heavy metal pollutions. Recent studies have shown that helminths parasites can be used as efficient environmental sentinels. This study aimed to evaluate Fasciola hepatica and Dicrocoelium dendriticum as bioaccumulators of lead (Pb), chromium (Cr), cadmium (Cd), copper (Cu). For this work. A total of 50 samples (F. hepatica, D. dendriticum, and livers from the infected and uninfected sheep, each of 10 samples) were collected from sheep slaughtered in Tabriz abattoir. One gram of each sample was incinerated and analyzed by Flame Atomic Absorption Spectrometry. The analysis of samples showed that Pb, Cr and Cu values in F. hepatica were higher than those in D. dendriticum, but only the differences of Pb and Cu were significant. The values of heavy metals in F. hepatica were significantly higher than those in the infected livers (except for Cd), while in D. dendriticum, Cr and Cd were only higher. Based on metal levels in livers, it was found that bioconcentration factors (BCFs) of Cr, Pb and Cu for F. hepatica were much more than one, and BCFs of these three metals between two flukes were statistically significant. This study indicated that F. hepatica had a higher bioindicator potential than D. dendriticum to evaluate environmental pollutants by some metals.
Tanvika Sangwan; Neetu Saini; Deepika Kataria
Volume 13, Issue 4 , December 2022, , Pages 615-619
Abstract
A one and a half years old male French bulldog weighing 9.50 kg was presented with the history of inappetence, lethargy, abdominal distension and exercise intolerance since last 2 days. The physical examination was done which revealed normal physiological parameters including temperature, mucus membrane ...
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A one and a half years old male French bulldog weighing 9.50 kg was presented with the history of inappetence, lethargy, abdominal distension and exercise intolerance since last 2 days. The physical examination was done which revealed normal physiological parameters including temperature, mucus membrane color and capillary refill time except palpable precordial thrills, jugular distension on palpation, tachycardia and systolic murmurs on auscultation. Electrocardiography (ECG) was done which depicted ectopic foci with atrioventricular junctional tachycardia and right ventricular enlargement involving very small inverted P waves, deep S waves in leads I, II, III and augmented vector foot (aVF) and splintered QRS complexes. The dog was undergone chest radiography that revealed right atrial enlargement, increased sternal contact of heart on lateral view and a bulge at 9:00 o’clock to 11:00 o’clock depicted right atrial enlargement on dorso-ventral view. Lastly, echocardiography was done to arrive at a diagnosis confirming the Ebstein’s anomaly as a form of tricuspid valve dysplasia including apical displacement of tricuspid valve leaflets, division of right ventricle into atrialized and functional portions, increased displacement index, increased apex-mitral annulus to apex-tricuspid annulus ratio, severe right atrial dilatation and tricuspid regurgitation. The dog was medically treated with diuretics, angiotensin converting enzyme inhibitors and inotropes and the owner was advised to put the dog on low sodium diet for 2 weeks. The dog has resolved clinical signs of right sided heart affection; but, suddenly collapsed at home. The owner denied for the necropsy of dog.
Shiva Amanollahi; Ali Mirshahi; Hossein Kazemi Mehrjerdi
Volume 13, Issue 4 , December 2022, , Pages 621-624
Abstract
The development of tooth is a complex process wherein there is a series of interactions between the ectoderm and ectomesenchyme. Hyperdontia (also called supernumerary teeth) is a developmental abnormality being additional to the normal series of teeth with unknown etiology and is rare in ruminants. ...
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The development of tooth is a complex process wherein there is a series of interactions between the ectoderm and ectomesenchyme. Hyperdontia (also called supernumerary teeth) is a developmental abnormality being additional to the normal series of teeth with unknown etiology and is rare in ruminants. In this report, a rare case with two abnormal teeth outside the alveolar region, observed in a healthy 3-month-old female lamb was presented. The congenital defect consisted of the presence of 2 teeth positioned bilaterally in the caudal part of the mandible ramus and under the prominent pendulous skin; so, with retraction of prominent pendulous skin, these ectopic teeth were seen outside of the skin. The anomaly was found during a routine physical examination and no other congenital abnormalities were found in the lamb. The etiology of supernumerary teeth is not completely understood. This odontogenic abnormality appears to be an extraordinarily rare condition. Genetic, toxic, infectious or environmental factors related to congenital abnormalities affect the tooth growth. Depending on the supernumerary teeth position, surgery may be required.
Guangwei Zhao; Xinzhu Deng; Di Wu; Ruibing Cao; Aojun Shao; Yu Zhou; Tianqi Zhang; Ge Li; Hongzhang He; Jing Lu; Liwu Zhang
Volume 13, Issue 1 , March 2022, , Pages 1-6
Abstract
The gosling gout, a newly emerged disease, has widely broken out in China since 2017. Typical signs for the disease include diarrhea, anorexia, depression, dehydration, emaciation and paralysis. At autopsy, uratosis was the main pathological change which could be found at kidney, pericardium, air sac, ...
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The gosling gout, a newly emerged disease, has widely broken out in China since 2017. Typical signs for the disease include diarrhea, anorexia, depression, dehydration, emaciation and paralysis. At autopsy, uratosis was the main pathological change which could be found at kidney, pericardium, air sac, muscle and leg joint. In this study, gosling gout was firstly diagnosed by metagenomic analysis. Samples of kidney, Fabricius bursa, spleen and jejunum were collected and submitted to next-generation DNA sequencing. Our results demonstrated that goose avastrovirus was highly related with this disease. We confirmed the sequencing results by reverse transcription polymerase chain reaction method and artificial infection experiment and got consistent results. In summary, metagenomic sequencing method combined with traditional molecular identification was applied toward diagnosis of a novel gosling gout disease in China and revealed that goose avastrovirus was highly related with this disease. It has been proved to be a powerful tool for rapid and sensitive diagnosis of animal diseases, especially for some exceptional pathogens. In addition, host range, variation, molecular pathogenesis and potential zoonotic infection of this novel goose astrovirus need to be further studied.
Emin Karakurt; Nüvit Coşkun; Enver Beytut; Ömer Faruk Keleş; Serpil Dağ; Volkan Yilmaz; Hilmi Nuhoğlu; Ayfer Yıldız; Emre Kurtbaş
Volume 14, Issue 1 , January 2023, , Pages 1-6
Abstract
In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine ...
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In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 - 2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 – 2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor-associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.
Hikmet Dinç; Akın Yiğin; İsmail Koyuncu; Mustafa Aslan
Volume 13, Issue 1 , March 2022, , Pages 7-14
Abstract
At the present time cancer is one of the biggest health problems and because of the problems encountered in its treatment, alternative treatment methods of herbal origin are researched. In this study, the cytotoxic effects of the essential oil extracted from the Micromeria congesta plant on various cancer ...
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At the present time cancer is one of the biggest health problems and because of the problems encountered in its treatment, alternative treatment methods of herbal origin are researched. In this study, the cytotoxic effects of the essential oil extracted from the Micromeria congesta plant on various cancer cells (A549, ECC-1, HCT-116, HELA, HGC-27, MDA-MB-231, SNU-423, U20S, DLD-1, PC-3) and normal cells (BEAS-2B, CRL-4010) have been examined. Anticancer mechanism of action has been particularly examined on gastric cancer (HGC-27; IC50: 15.84 µg mL-1), on which essential oil showed a high cytotoxic effect. In the study, the cytotoxic effect and the apoptotic effect have been applied by MTT and flow cytometric annexin-V methods, respectively. The apoptotic gene expression (caspase 3, caspase 9, MMP2, MMP9, ACTB) real-time PCR content analysis has been performed with gas chromatography mass spectrometry (GC-MS). M. congesta essentials oil has the highest cytotoxic effect on gastric cancer (HGC-27) cells, decreases MMP2 and MMP9 expressions, and induces apoptosis with increasing the expression of caspase 3 and caspase 8 genes. In addition, it has been determined that piperitenone oxide (40.00 - 45.00%), pulegone (11.00%) and cyclohexanone (18.00 - 19.00%) are the major components of M. congesta essentials oil. In conclusion, it has been determined that the compounds found in high amounts in M. congesta plant induces apoptosis by affecting the expression of compound genes and thus can have the potential to be an alternative drug in the treatment of gastric cancer.
Jun-Ting Liu; Yan-Hui Chen; Yi-Feng Pei; Qian Yu; Ruth Afumba; Hao Dong
Volume 14, Issue 1 , January 2023, , Pages 7-12
Abstract
Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May 2020 and applied it to the loop-mediated ...
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Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May 2020 and applied it to the loop-mediated isothermal amplification (LAMP) assay. We designed five sets of primers for the goose parvovirus VP3 gene by LAMP. The GV-1 primer set was selected to detect GPV sensitively and rapidly. LAMP was more sensitive compared to PCR. In addition, the LAMP method could complete detection within 60 min which was faster than the PCR assay. The LAMP provided a convenient and effective experimental method for detection of GPV for inspection and quarantine departments and health care units in China, and it is expected to become a simple and routine detection method, especially suitable for goose farms.
Vasileios Papatsiros; Georgios Papakonstantinou; Eleftherios Meletis; Dimitra Bitchava; Polychronis Kostoulas
Volume 14, Issue 1 , January 2023, , Pages 13-19
Abstract
The objective of the present study was to evaluate the efficacy of a porcine circovirus type 2 (PCV2) double vaccination in weaning piglets reared for gilts under field conditions. The study was conducted at a Greek farrow-to-finish conventional pig farm with a previous history of PCV2 infections. The ...
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The objective of the present study was to evaluate the efficacy of a porcine circovirus type 2 (PCV2) double vaccination in weaning piglets reared for gilts under field conditions. The study was conducted at a Greek farrow-to-finish conventional pig farm with a previous history of PCV2 infections. The trial included 96 female piglets at 21 days of age, which were equally allocated to two different study groups. Piglets of the group-1 received a single PCV2 vaccination at 21 days of age, while piglets of the group-2 were double vaccinated against PCV2, at 21 and 42 days of age. The results indicated that the piglets of group-2 had better growth performance, as they showed higher body weight (BW) and average daily weight gain (ADWG). In addition, ELISA tests showed that the double- vaccinated piglets presented a better humoral response against PCV2, as higher levels of IgG antibodies were detected in them than the piglets of the group-1. In conclusion, the current results suggested that a double PCV2 vaccination of piglets, reared for gilts, on a PCV2-affected farm could lead to higher protection against the virus.
Tahir Karaşahin; Nese Hayat Aksoy; Şükrü Dursun; Gaye Bulut; Ali Evren Haydardedeoğlu; Güzin Çamkerten; İlker Çamkerten; Ramazan İlgün
Volume 13, Issue 1 , March 2022, , Pages 15-19
Abstract
This study was performed to determine the blood reference values of hematological and biochemical parameters in Hair goats at different ages. For this aim, clinically healthy Hair goats (n = 180, ages of six month and 1-3 years male and female) were used to collect blood samples. In whole blood, white ...
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This study was performed to determine the blood reference values of hematological and biochemical parameters in Hair goats at different ages. For this aim, clinically healthy Hair goats (n = 180, ages of six month and 1-3 years male and female) were used to collect blood samples. In whole blood, white blood cell (WBC), red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)and red cell distribution width (RDW) were evaluated. The levels of, albumin, total protein, globulin, glucose, total bilirubin, urea, creatinine, phosphorus, magnesium, calcium, aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), creatine kinase (CK), alanine aminotransferase (ALT) and iron levels were investigated in sera. Age and sex had no effect on the cell structures of blood. In the either sex (male or female), age had no effect. However, sex had a significant effect on the blood cell structures except for WBC, HGB, and HCT. Albumin, protein, calcium, AST, ALT, ALP and magnesium values were not different among groups. Both the age and sex of the goats had significant effects on blood levels of glucose, phosphorus, urea, cholesterol, creatinine, GGT, CK and total bilirubin. In conclusion, this was the first study reporting the hematologic and biochemical parameters in blood of the Hair goats. The results indicated that these parameters could show variations under physiological conditions due to age and gender.
Ümit Gürbüz; Hatice Ahu Kahraman; Arife Ezgi Telli; Yusuf Biçer; Yusuf Doğruer
Volume 13, Issue 1 , March 2022, , Pages 21-27
Abstract
The purpose of the present study was to investigate the physicochemical characteristics, meat quality, oxidative stability and sensory properties of lamb meat during 0, 7 and 14 day of the dry aging process. The M. longissimus lumborum (LL) and M. longissimus thoracis (LT) muscles from male Akkaraman ...
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The purpose of the present study was to investigate the physicochemical characteristics, meat quality, oxidative stability and sensory properties of lamb meat during 0, 7 and 14 day of the dry aging process. The M. longissimus lumborum (LL) and M. longissimus thoracis (LT) muscles from male Akkaraman lambs were used. The pH values of the LT and LL cuts were not changed during the aging periods. The LT cuts had significantly higher weight loss, a* and b* values, and lower shear force compared to the LL cuts. However, dry aging led to greater decreases in shear force in the LL cuts on 7th day of aging. The total mesophilic aerobic counts, total psychrophilic counts, Enterobacteriaceae counts, lactic acid bacteria, and yeast-mold counts were increased during the aging process. The sensory panel scoring showed a significant difference in the LL cuts and no significant difference in the LT cuts compared to the control group. There were significant changes in sensory panel scores for the LL cuts, whereas there were no significant changes for the LT cuts according to the non-aged samples. In conclusion, dry aging improved the quality of both cuts, however, the LL muscle of lamb was more suitable for dry aging. Moreover, 7 days were sufficient to produce the desired sensory properties in the lamb loins. Increasing the aging time from 7 to 14 days did not appreciably affect the sensory attributes or tenderness.
Abdulghaffar Ownagh; Navid Etemadi; Peyman Khademi; Hossein Tajik
Volume 14, Issue 1 , January 2023, , Pages 21-28
Abstract
Salmonellosis is one of the most important bacterial diseases in human and animals. Rapid diagnosis and sub sequence accurate treatment of Salmonella carriers help reduce the salmonellosis in human and livestock animals. In this study, 420 fecal samples were taken during year 2019 from buffalo in the ...
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Salmonellosis is one of the most important bacterial diseases in human and animals. Rapid diagnosis and sub sequence accurate treatment of Salmonella carriers help reduce the salmonellosis in human and livestock animals. In this study, 420 fecal samples were taken during year 2019 from buffalo in the Urmia, Khoy and Piranshahr regions in west Azerbaijan province, Iran. Samplings were carried out in different seasons. Presence of Salmonella invasion genes (FimA, Stn and InvA) were evaluated by polymerase chain reaction. The bacterial culture and biochemical tests were performed on feces samples for isolation of bacterium Salmonella; however, all samples were negative in culture method. PCR findings showed that, 50 (11.90%) fecal samples were positive to the genes. The analysis of results showed that frequency of salmonellosis outbreak in different parts of west Azerbaijan province followed a similar pattern and the incidence of salmonellosis according to forecast in the warm seasons (spring and summer) was more than in cold seasons (autumn and winter). The prevalence of Salmonella in buffalo’s feces based on warm and cold seasons were 32 (64.00%) and 18 (36.00%), respectively. The results showed significant difference between cold and warm season in the prevalence of salmonellosis. Therefore, the application of molecular technics is essential for the prevention and treatment of salmonellosis. The results also showed that specificity of PCR method was better than culture method for detection of Salmonella in feces sample.
Masoud Ahmadnejad-Asl-Gavgani; Masoud Maham; Bahram Dalir-Naghadeh
Volume 13, Issue 1 , March 2022, , Pages 29-37
Abstract
Tanacetum balsamita (Asteraceae) is a medicinal plant previously used in human medicine to solve gastrointestinal problems such as abdominal pain. Anti-inflammatory, analgesic, immune-modulatory, and antioxidant effects of T. balsamita have been proven in previous studies. The present study investigated ...
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Tanacetum balsamita (Asteraceae) is a medicinal plant previously used in human medicine to solve gastrointestinal problems such as abdominal pain. Anti-inflammatory, analgesic, immune-modulatory, and antioxidant effects of T. balsamita have been proven in previous studies. The present study investigated the effects of T. balsamita essential oil (TBEO) on ruminant smooth muscle contractions. The experiment was performed on the circular smooth muscle of ileum samples taken from slaughtered bulls in the organ bath. Nine cumulative concentrations of TBEO from 0.10 to 1000 µg mL-1 were added to tissue samples. The solution used was Tyrode’s solution aerated with a mixture of 95.00% oxygen and 5.00% carbon dioxide, and the temperature was set at 37.00 ˚C. The effect of TBEO on baseline contractions and three induced contractions with potassium chloride, barium chloride, and carbachol was investigated. In GC-MS analysis of TBEO, carvone was identified as a major ingredient. The effects of eight concentrations of 0.001 to 10.00 µM of carvone on all contractions were investigated under similar conditions. The effects of TBEO, carvone, and verapamil (standard calcium channel blocker) on calcium channels were assessed. The results revealed that TBEO and carvone significantly inhibit spontaneous contractions as well as all spasmogen-induced contractions. The TBEO and carvone exert their myorelaxant properties by inhibiting Ca++ channels in smooth muscle. The anti-spasmodic properties of T. balsamita can be employed for the treatment of intestinal spasms or hypermotility.
Gholam Abbas Ghorbankhani; Ali Mohammadi; Nasrin Kazemipour; Solmaz Morovati; Behrouz Gharesi Fard; Saeed Nazifi Habibabadi; Mahdi Hashempour Sadeghian
Volume 14, Issue 1 , January 2023, , Pages 29-37
Abstract
Given the development of drug-resistant cancer cells, designing alternative approaches for cancer treatment seems essential. In this study, we evaluated the anti-tumor effects of nisin A and newcastle disease virus (NDV) on triple-negative MDA-MB-231 cell line. The MDA-MB-231 cell line was separately ...
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Given the development of drug-resistant cancer cells, designing alternative approaches for cancer treatment seems essential. In this study, we evaluated the anti-tumor effects of nisin A and newcastle disease virus (NDV) on triple-negative MDA-MB-231 cell line. The MDA-MB-231 cell line was separately and in combination subjected to the different concentrations of a Vero-adapted NDV (JF820294.1) and nisin A. The oncolytic effects of these treatments were analyzed by different cytotoxic and apoptosis techniques including trypan blue staining, MTT assay, acridine orange (EB/AO) staining, colony assay and flow cytometry over time. Nisin A at doses of more than 20.00 μg mL-1 could represent the anti-viral effects and interfere with the oncolytic activity of NDV. Moreover, the analyses indicated that the anti-proliferative and cytotoxic features of combination therapy were stronger than those of individual NDV groups. However, the most apoptotic effect was seen in NDV experimental groups. Taken together, the results from cytotoxicity tests, flow cytometry and colony assay showed that either of the oncolytic agents had significant effects at low concentrations 72 hr post-treatment. Thereby, they had the potential to be used as new approaches in cancer treatment.
Mohammad Reza Esmaili Nejad; Majid Masoudifard; Omid Azari; Ehsanollah Sakhaee; Nima Ghalekhani
Volume 13, Issue 1 , March 2022, , Pages 39-46
Abstract
Echocardiography illustrates a convenient and noninvasive tool for measuring cardiac output (CO) changes after administration of sedative drugs, but it is unknown in camelids practice. The aim of present study was to investigate echocardiographic effects of intravenous (IV) injection of medetomidine ...
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Echocardiography illustrates a convenient and noninvasive tool for measuring cardiac output (CO) changes after administration of sedative drugs, but it is unknown in camelids practice. The aim of present study was to investigate echocardiographic effects of intravenous (IV) injection of medetomidine and xylazine in camel calves. Twenty apparently healthy immature male one-humped camel calves (Camelus dromedarious) were divided into four groups (five animals in each treatment). Medetomidine and xylazine were injected into the left jugular vein at two different doses of 10.00 and 20.00 μg kg-1 and 0.20 and 0.40 mg kg-1, respectively. Effects on some selected echocardiographic parameters were recorded at different intervals, before drug administrations (baseline) and after 3, 60 and 120 min. Data were analyzed by repeated measure, ANOVA test, then relevance and significance were taken as p ≤ 0.05. Significant decrease in fractional shortening percentage (FS%), ejection fraction percentage (EF%), stroke volume (SV), heart rate (HR) and subsequent CO were noticeable 3 min after drug administration in medetomidine high dose (MH), medetomidine low dose (ML) and xylazine high dose (XH) groups (p ≤ 0.05), furthermore at this time significant decrease in left ventricular mass (LVmass) and left ventricular systolic time intervals were seen in these groups, however, in xylazine low dose (XL) group, the lowest level of most echocardiographic parameters were detectable after 60 min. High dose IV injection of medetomidine was associated with significant decrease in most echocardiographic parameters without echocardiographic arrhythmia. Although, ML and XH groups had the same effects on echocardiographic indices but the intensity and duration were less than MH group.
Fahimeh Firouzjaie; Vahideh Moin-Vaziri; Alireza Zahraei Ramezani; Hamed Behniafar; Mehdi Badakhshan; Adel Spotin; Zabih Zarei
Volume 14, Issue 1 , January 2023, , Pages 39-43
Abstract
Leishmaniasis is a protozoal and vector-borne disease. World health organization has considered the disease as a neglected tropical disease. Phlebotomus and Lutzumyia species (order: Diptera, family: Psychodidae) are human leishmaniasis vectors in new and old worlds. Sergentomyia spp. (Diptera, Psychodidae) ...
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Leishmaniasis is a protozoal and vector-borne disease. World health organization has considered the disease as a neglected tropical disease. Phlebotomus and Lutzumyia species (order: Diptera, family: Psychodidae) are human leishmaniasis vectors in new and old worlds. Sergentomyia spp. (Diptera, Psychodidae) are proven vectors of lizard leishmaniasis. Although some studies have identified human Leishmania parasites in Sergentomyia, their role in parasite circulation is unknown yet. Hence, the parasitological and molecular methods were used to study the possible Leishmania infection of Sergentomyia spp., in the human and canine visceral leishmaniasis endemic area in North West of Iran. Even though Sergentomyia specimens were caught in a dominant number compared to Phlebotomus spp., no Leishmania promastigote or DNA was detected in live-caught or sticky trap-caught specimens, respectively. Sergentomyia spp. are proven vectors of sauroleishmaniasis, and despite several global reports of Leishmania infection in Sergentomyia spp., such findings should be carefully interpreted to avoid false vector incriminations.
Parisa Gheibi; Zohre Eftekhari; Delaram Doroud; Kazem Parivar
Volume 14, Issue 1 , January 2023, , Pages 45-52
Abstract
Various factors are effective in reducing the fertility rate. This experiment aimed to investigate chlorpyrifos (CPF), an organophosphate, that could alter the structure of the uterus and the molecules involved in parental and fetal. CPF was injected intraperitoneally in thirty mice for five days in ...
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Various factors are effective in reducing the fertility rate. This experiment aimed to investigate chlorpyrifos (CPF), an organophosphate, that could alter the structure of the uterus and the molecules involved in parental and fetal. CPF was injected intraperitoneally in thirty mice for five days in a week (six weeks). The animals were euthanized on the 5th day of gestation, then their blood and uterus were collected for biochemical and histopathological assays. Exposure to CPF resulted in a significant reduction in maternal weight gain and the number of litters. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were significantly increased in blood serum of the CPF group compared with the control. The number of uterus glands, endometrium thickness, and the uterine cavity were changed following CPF injection. Additional investigation indicated that the expressions of L-selectin, L-selectin ligand, and heparin-binding epidermal growth factor (HB-EGF) as initial adhesion of mice blastocysts and maternal endometrium biomarkers were downregulated in the CPF group. Nevertheless, any mortality and abnormal clinical symptoms were not observed in the treated mice. This study revealed a potential molecular mechanism of continuous CPF-induced toxicity in fetal-maternal attachment without clinical symptoms.
Nasser Darban Maghami; Abolghasem Nabipour; Mohammad Mohsenzadeh; Maryam Torabi
Volume 13, Issue 1 , March 2022, , Pages 47-53
Abstract
Meat and meat products are highly important sources of protein in the diet. Nowadays, the consumption of meat and meat products has increased owing to modern manufacturing techniques. Due to the economic value of meat, the use of unauthorized tissue is possible in meat products. In some cases, there ...
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Meat and meat products are highly important sources of protein in the diet. Nowadays, the consumption of meat and meat products has increased owing to modern manufacturing techniques. Due to the economic value of meat, the use of unauthorized tissue is possible in meat products. In some cases, there is fraud in the percentage of meat in meat products to reduce prices. In this study, 34 samples of minced meat, hamburger and sausage were randomly collected from the markets in the northeast of Iran. Then, sections were stained using Hematoxylin and Eosin (H & E), Verhoeff-van-Gieson, Masson's trichrome and periodic acid–Schiff-Alcian blue stains. In this regard, for the first time, the efficacy of stereological technique to determine the percentage of meat listed in sausages and the possible existence of fraud was evaluated. The results showed that, due to the presence of some unusual tissues, histological technique could determine different tissues in meat products. The stereological results of control samples showed a very slight difference; whereas, the results for the samples collected from the city stores showed a distinctive difference regarding the percentage of meat compared to the percentage of label. Skeletal and smooth muscles, blood vessels, nerve, gizzard, adipose tissue, glandular tissue, cartilage, bone, tendon, skin, lymphatic tissues and plant materials were observed. It was confirmed that stereology was a reliable method to determine and confirm the percentage of meat used in meat products.
Seyed Hesamaddin Banihashemi; Amir Saeed Samimi; Kamyab Samimi; Javad Tajik
Volume 14, Issue 1 , January 2023, , Pages 53-57
Abstract
The intravenous (IV) usage of diuretics such as furosemide may cause changes in clinical variables, electrocardiographic (ECG) indices and some serum electrolytes concentrations in miniature donkeys. The purpose of this study was to evaluate the effects of double intravenous (IV) administration of furosemide ...
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The intravenous (IV) usage of diuretics such as furosemide may cause changes in clinical variables, electrocardiographic (ECG) indices and some serum electrolytes concentrations in miniature donkeys. The purpose of this study was to evaluate the effects of double intravenous (IV) administration of furosemide at different doses on the clinical variables, ECG indices and serum electrolytes levels in miniature donkeys. A total of 21 clinically and para-clinically healthy miniature donkeys with a mean age of 6.00 ± 1.00 years and weight of 95.00 ± 5.00 kg were used. The animals were randomly divided into three groups of control (normal saline) and low- (1.00 mg kg-1) and high-dose (2.00 mg kg-1) of furosemide. Two IV injections with 12-hr interval were administered in each animal. The clinical and ECG parameters were measured at 0 (baseline), 2 (T2), 24 (T24) and 48 (T48) hr after drug administration. Serum concentrations of electrolytes were measured at T0, T24 and T48. The results of this study showed no changes in clinical variables (heart rate, rectal temperature and respiratory rate) and ECG indices (amplitude and duration of P, QRS and T waves and duration of PR, QT and RR intervals) as well as no significant changes in serum electrolytes (sodium, potassium, chloride, phosphorus, calcium and magnesium) levels. Although no significant effect on clinical variables, electrolytes levels and ECG indices after double IV administration of furosemide (1.00 and 2.00 mg kg-1) in miniature donkeys was observed, absence of these effects does not mean that furosemide had no pharmacological effect.
