Shirzad Ghaderzadeh; Farzad Mirzaei Aghjehgheshlagh; Saeid Nikbin; Bahman Navidshad
Volume 11, Issue 4 , December 2020, , Pages 385-391
Abstract
Sheep keepers need suitable strategies to improve animal immunity and the quality of their products. This study was aimed to evaluate the effect of nano-selenium (nano-Se) and conjugated linoleic acid (CLA) on an antioxidant statue, trace minerals, and mRNA expression of glutathione peroxidase 1 (GPX1) ...
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Sheep keepers need suitable strategies to improve animal immunity and the quality of their products. This study was aimed to evaluate the effect of nano-selenium (nano-Se) and conjugated linoleic acid (CLA) on an antioxidant statue, trace minerals, and mRNA expression of glutathione peroxidase 1 (GPX1) and selenoprotein W1 (SEPW1) genes in the liver and peroxisome proliferator-activated receptor-gamma (PPARγ) and stearoyl COA desaturase 1 (SCD1) genes in fat- tail of male Moghani lambs. Thirty male Moghani lambs, three months old and average weight 30.00 ± 0.25 kg, were assigned to a completely randomized design in a 2×3 factorial arrangement with dietary supplementation of nano-Se (0, 1.00 and 2.00 mg kg-1 dry matter) and CLA (0 and 15.00 g kg-1 dry matter). The lambs were slaughtered at the end of the experiment, on day 90 of the experiment. Results showed that dietary inclusion of nano-Se significantly improved antioxidant enzymes glutathione peroxidase and superoxide dismutase in blood, however, did not show any differences in trace mineral treatments. The analysis of qPCR showed that nano-Se inclusion at the highest level (2.00 g kg-1 dry matter) enhanced gene expression of GPX1 (0.64 vs 0.34) and SEPW1 1 (0.72 vs 0.35) in the liver. Dietary inclusion of CLA increased the expression of PPARγ (0.63 vs 0.38) and decreased SCD1 (0.63 vs 0.33) genes in fat- tail. It could be concluded that selenium inclusion in the growing lamb’s diet could improve antioxidant status, however, no synergistic interaction was observed along with CLA on the mentioned parameters.
Mehdi Golchin; Somayye Mollayi; Elham Mohammadi; Neda Eskandarzade
Volume 13, Issue 3 , September 2022, , Pages 387-391
Abstract
Brucellosis is considered as one of the important global zoonotic diseases that causes medical as well as economic problems especially in tropical countries. The illness has no specific pathognomonic signs; therefore, the rapid and accurate diagnosis of the disease has a very important role in preventing ...
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Brucellosis is considered as one of the important global zoonotic diseases that causes medical as well as economic problems especially in tropical countries. The illness has no specific pathognomonic signs; therefore, the rapid and accurate diagnosis of the disease has a very important role in preventing the Brucella spillover and treatment. The purpose of this study was to design a new indirect ELISA test for detection of human brucellosis based on using recombinant Brucella abortus outer membrane protein 16.00 kDa (rOMP16) as an antigen. OMP16 gene of B. abortus was initially synthesized and cloned in pET-21d vector and then expressed in Escherichia coli cells. The expression was confirmed by the SDS-PAGE, western blotting and dot blotting. The purified protein was coated in ELISA plates and an indirect ELISA was performed on 70 human serum samples. The results were evaluated with a commercial IgG ELISA kit and Rose Bengal plate agglutination tests as reference tests. Diagnostic performance of designed OMP16 ELISA test in comparison with Rose Bengal plate test revealed 100% of sensitivity, 95.00% of specificity and good Fleiss kappa agreement, whereas, where it was compared to commercial ELISA kit, it revealed very good kappa agreement with 100% of sensitivity and 100% of specificity in cut-off value of 0.13. It was concluded that OMP 16.00 kDa could be acceptable alternative antigen for detecting Brucella IgG antibody with high accuracy.
Surgery
Emad Vakili-Sadeghi; Alireza Najafpour; Rahim Mohammadi
Volume 14, Issue 7 , July 2023, , Pages 389-395
Abstract
The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: ...
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The torsion model of testis in a rat was adopted for evaluation of possible effects of propolis (Prop) on ischemia-reperfusion (IS/REP) injury. The healthy male Wistar rats (totally 24 animals) were randomized into four groups (n = 6) and animals experienced bilateral testicular torsions as follows: In sham group just, laparotomy was performed and in IS group, animals experienced a 3 hr period testicular IS. In IS/REP group, a 3 hr period of IS followed by a 3 hr period of testicular REP for left testis and a one-week testicular REP for right testis were done. In this group animals were gavaged by 1.00 mL normal saline 1 hr before the onset of IS. In IS/REP/ Prop group, the same procedures for IS/REP animals were followed as well as gavage of 1.00 mL Prop extract solution 1 hr before the onset of IS. Analyses of biochemistry, histology, inflammatory biomarkers and sperm parameters were carried out. In IS/REP/Prop group, nitric oxide synthase malondialdehyde, myeloperoxidase and 8-hydroxy-2 deoxyguanine in IS/REP/Prop group were significantly decreased and, superoxide dismutase, total glutathione, glutathione peroxidase, glutathione reductase and glutathione S-transferase were significantly increased compared to the other animals. In IS/REP/Prop group, seminiferous tubules (with normal spermatogenesis) showed all stages of spermatogenic cells with plentiful spermatozoa. Tubular deterioration and atrophy and spermatogenic cell loss in were seen in a limited extent. The mean concentrations of Interleukin-1 beta and tumor necrosis factor alpha in IS/REP/Prop were significantly decreased. Sperm quality was significantly improved by Prop in IS/REP/Prop group. It was concluded that Prop could be supportive in diminishing IS/REP injury in testicular tissue exposed to ischemia.
Asad Pormohammad; Tahereh Mohammadabadi; Morteza Chaji; Khalil Mirzadeh; Jasem Ghafle Marammazi
Volume 11, Issue 4 , December 2020, , Pages 393-399
Abstract
The aim of this study was to evaluate the effect of biofloc produced from a fish pond on digestibility, rumen fermentation, rumination activity, and blood parameters of Najdi goats. At the first, 200 common carp fish (1.00 g) in two water tanks were used for producing biofloc. Then, 12 goats (average ...
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The aim of this study was to evaluate the effect of biofloc produced from a fish pond on digestibility, rumen fermentation, rumination activity, and blood parameters of Najdi goats. At the first, 200 common carp fish (1.00 g) in two water tanks were used for producing biofloc. Then, 12 goats (average body weight of 32.00 ± 1.50 kg aging eight months) were assigned through a completely randomized design to two treatments (six replicates) and fed for one month. The treatments were comprised of a control diet and a trial diet containing 1.50% biofloc. At the end of the experiment, digestibility of nutrients, rumen fermentation, blood metabolites, and rumination activity were measured. The results showed that the digestibility of dry matter (DM), crude protein, neutral detergent fiber (NDF), and acid detergent fiber (ADF) significantly increased in goats fed with 1.50% biofloc. Also, the rumen protozoa population, plasma glucose, average time of eating, rumination, and chewing per NDF and ADF intakes, total rumination time, and chewing activity were significantly increased. There was no significant difference between treatments for DM intake, ruminal ammonia nitrogen, blood urea nitrogen, cholesterol, and triglyceride. The pH of rumen fluid significantly decreased in treatment containing biofloc. The results of the current study confessed that the use of biofloc (1.50% DM) as a supplement in Najdi goat’s diet can improve the digestibility of the diet. It is recommended further and detailed trials with different levels of biofloc in sheep or different livestock species to know more about this untapped supplement source.
Katayoon Nofouzi; Parsa Almasi; Ali Asghar Fakhri-Demeshgheieh; Monireh Khordadmehr; Behzad Baradaran; Milad Asadi; Parvin Sarbakhsh; Gholamreza Hamidian
Volume 13, Issue 3 , September 2022, , Pages 393-401
Abstract
Breast cancer (BC) is a significant cause of global mortality in women. This study was aimed to evaluate the immune-activation of malignant BC via the administration of attenuated Mycobacterium obuense. For this purpose, an in vivo model was developed with BALB/c mice. Mice were injected with 2.00 × ...
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Breast cancer (BC) is a significant cause of global mortality in women. This study was aimed to evaluate the immune-activation of malignant BC via the administration of attenuated Mycobacterium obuense. For this purpose, an in vivo model was developed with BALB/c mice. Mice were injected with 2.00 × 106 4T1 cells with breast tumor cell line. Forty-two mice were equally divided into control as well as low dose (0.20 mg 100 µL-1) and high dose (0.50 mg 100 µL-1) groups of M. obuense to investigate gene expression in the antitumor effects of M. obuense. In one group, paclitaxel was administrated as a choice drug in BC treatment. Antitumor manners were characterized by cytotoxicity against tumor target cells, size of the tumor and the expression of some BC metastatic genes together with pathology. The MTT assay demonstrated that different concentrations of both low and a high doses of bacteria did present no cytotoxicity effect on 4T1 cells. According to our findings, M. obuense significantly repressed tumor growth. M. obuense downregulated the expression of collagen type I alpha 1 (COLIA1), cFos, alkaline phosphatase (ALP), claudin 3 (cldn3), and conversely, activated transcription factor 4 (ATF4) and Twist related protein-1 (Twist1). All these alternations induced a decrease in the migratory and invasive capabilities of BC. The result of pathology was indicative of tumor regression in the paclitaxel and HK- M. obuense -recipient group. Thus, it seems most likely that M. obuense might impinge upon cell growth and metastatic behavior of malignant cells exerting anti-tumor activity in BC.
Iftar Gürbüz; Yasin Demiraslan
Volume 12, Issue 4 , December 2021, , Pages 395-399
Abstract
In the present study the comprisal of brachial plexus and nerves springing from the brachial plexus in Günther’s vole has been determined. Ten adult voles (five males and five females) were used in this study. Nerves comprising the brachial plexus and the nerves arising from brachial plexus ...
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In the present study the comprisal of brachial plexus and nerves springing from the brachial plexus in Günther’s vole has been determined. Ten adult voles (five males and five females) were used in this study. Nerves comprising the brachial plexus and the nerves arising from brachial plexus in vole were dissected and the findings were saved. The brachial plexus of the vole was comprised of the ventral root of cervical (C) 5th, 6th, 7th and 8th and thoracic (T) 1st spinal nerves. Also, the brachial plexus was consisted of three trunks including cranial (C5th and C6th), medial (C7th) and caudal (C8th and T1st) trunks. Nervus (n.) subclavius, n. suprascapularis, nervi (nn.) subscapularis, n. axillaris, n. musculocutaneus, n. thoracodorsalis, nn. pectorales craniales, n. pectorales caudales, n. thoracicus lateralis, n. thoracicus longus, n. ulnaris, n. medianus, and n. radialis were determined to be arising from brachial plexus. In conclusion, macroanatomical features of brachial plexus and its branches in Günther’s vole have been determined. It is thought that the findings will contribute to neuroanatomy of the rodents.
Theriogenology
Héctor Javier Narváez; Luis Ernesto Quintero Rodríguez
Volume 14, Issue 7 , July 2023, , Pages 397-400
Abstract
The creole breed Blanco Orejinegro (Bos taurus adapted) is an important animal genetic resource in Colombia. However, very little is known about the reproductive physiology of the breed. The objective was to determine the dynamics of progesterone circulation and follicular growth in Blanco Orejinegro ...
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The creole breed Blanco Orejinegro (Bos taurus adapted) is an important animal genetic resource in Colombia. However, very little is known about the reproductive physiology of the breed. The objective was to determine the dynamics of progesterone circulation and follicular growth in Blanco Orejinegro cows throughout the estrous cycle. Fifteen cyclic Blanco Orejinegro cows were used and subjected to hormonal protocol for estrous and ovulation synchronization. Once the time of ovulation was identified by monitoring the ovarian dynamics with ultrasonography equipment. For description of the results, the mean was used as a measure of central tendency and the standard error of the mean. Statistical analysis was longitudinal descriptive. Blood samples were collected every 24 hr throughout the estrous cycle for serum quantification of progesterone (P4), monitoring of corpus luteum (CL) growth dynamics and follicular dynamics. It was possible to verify that 76.92% of the Blanco Orejinegro cows presented two follicular waves during the estrous cycle and the highest circulating levels of P4 (> 6.00 ng mL-1) were observed on the 14th day of the estrous cycle. It was concluded that the creole cows of the Blanco Orejinegro breed presented two follicular waves per estrous cycle. In addition to, the size of the CL was consistent with the secretion of progesterone presenting higher circulating levels at the end of the luteal phase.
Bahman Keramati Khiarak; Mojtaba Karimipour; Abbas Ahmadi; Gholam Hossein Farjah
Volume 11, Issue 4 , December 2020, , Pages 401-408
Abstract
Titanium dioxide particles (TiO2) as the second most widely used materials in consumer products are composed of nano-sized (100 nm) particles (FPs). Toxicological studies on animals have shown that TiO2 NPs exposure can cross the blood-testis barrier and accumulate in the testis resulting in testicular ...
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Titanium dioxide particles (TiO2) as the second most widely used materials in consumer products are composed of nano-sized (100 nm) particles (FPs). Toxicological studies on animals have shown that TiO2 NPs exposure can cross the blood-testis barrier and accumulate in the testis resulting in testicular tissue damage and reduction of sperm count and motility. However, there is no information on the toxic effects of TiO2 FPs on male reproductive fertility. Twenty-four adult male mice were randomly divided into three groups including control, TiO2 NPs, and TiO2 FPs (150 mg kg-1 per day). After intragastric administration for 35 days, testicular tissue alterations (seminiferous tubule diameter and germinal epithelial height), sperm parameters (count, motility, viability, morphology, and DNA quality), in vitro fertilization potential, oxidative stress assays such as malondialdehyde (MDA) content, level of glutathione (GSH) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in testicular tissue were investigated. The results showed that both sizes of TiO2 caused pathologic changes in the testis and significantly increased MDA level and decreased GSH levels and activities of SOD and GPx in testicular tissue. Moreover, the administration of both sizes of TiO2 significantly decreased all of the sperm parameters and in vitro fertility (fertilization rate and pre-implantation embryos development) compared to control. Administration of TiO2 FPs similar to TiO2 NPs through inducing damages to testis led to a marked reduction in sperm quality, in vitro fertilization, and embryos development in male mice.
Mohadeseh Barazandeh; Khashayar Shahin; Abolghasem Hedayatkhah; Majid Komijani; Mojtaba Mansoorianfar
Volume 12, Issue 4 , December 2021, , Pages 401-407
Abstract
Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of ...
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Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.
Large Animal Internal Medicine
Vasileios Georgios Papatsiros; Ioanna Stylianaki; Constantina Nikolaos Tsokana; Georgios Papakonstantinou; Marios Christophorou; Nikolaos Papaioannou; Labrini Vasileios Athanasiou
Volume 14, Issue 7 , July 2023, , Pages 401-404
Abstract
This study aimed to report, for the first time, histopathological lesions caused by an outbreak of acute Actinobacillus pleuropneumoniae serotype 8 infections in two farms in Cyprus. Lung tissue samples were collected from two different affected farms (a total of eight samples) for bacterial culture, ...
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This study aimed to report, for the first time, histopathological lesions caused by an outbreak of acute Actinobacillus pleuropneumoniae serotype 8 infections in two farms in Cyprus. Lung tissue samples were collected from two different affected farms (a total of eight samples) for bacterial culture, multiplex polymerase chain reaction (PCR)-based serotyping and histopathological evaluation. Severe respiratory clinical signs, vomiting, anorexia, sudden deaths, a morbidity rate of around 25.00% and a mortality rate of over 60.00% in the fattening stage were reported. Macroscopic lesions included acute to subacute fibrotic, hemorrhagic and necrotizing pneumonia with occasionally encapsulated nodule-like abscesses and fibrous pleuritis. Histopathological evaluation revealed fibrous exudate between alveolar spaces and connective tissue, areas of necrosis mixed with alveolar macrophages, lymphocytes, plasma cells and necrotic leukocytes surrounding colonies of cocci. The bronchial and bronchiolar epithelia were degenerated and replaced by eosinophilic cell debris mixed with inflammatory cells. Several arteries and capillaries were clotted and/or infiltrated by inflammatory cells. In conclusion, these A. pleuropneumoniae serotype 8 cases were accompanied by acute illness, death and more pronounced bronchitis and bronchiolitis.
Masoud Reza Seyfi Abad Shapouri; Pezhman Mahmoodi; Masoud Ghorbanpour Najafabadi; Mohammad Rahim Haji Hajikolaei; Parastoo Moradi Choghakabodi; Mohsen Lotfi; Mahdi Pourmahdi Boroujeni; Maryam Ekhtelat; Maryam Daghari
Volume 13, Issue 3 , September 2022, , Pages 403-407
Abstract
Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS3), as a single ELISA recombinant ...
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Diagnosis of bovine viral diarrhea (BVD) relies on the detection of antibodies against its viral causing agent, bovine viral diarrhea virus (BVDV). Here, we designed a novel competitive ELISA (cELISA) using the most immunogenic part of BVDV nonstructural protein 3 (NS3), as a single ELISA recombinant antigen, along with a monoclonal antibody to detect antibodies against BVDV in sera of infected animals. Hence, 197 serum samples were tested by this cELISA and the results were compared to the results obtained from virus neutralization test (VNT) as the gold standard method for diagnosis of BVD. McNemar’s test indicated that there was no significant difference between the results of this newly designed cELISA and VNT. Meanwhile, kappa coefficients showed that there was a high correlation between these two assays. The relative sensitivity and specificity of cELISA with respect to VNT were 93.90% and 100%, respectively, suggesting that this newly designed cELISA could be a useful diagnostic tool for detection of BVDV infection. Moreover, as NS3 is highly conserved among Pestiviruses and the developed ELISA is a competitive one, it could potentially be applied to detect BVDV infection in other domestic and wildlife species.
Sharanya Jeevendra Naidu; Arangasamy Arunachalam; Akeem Babatunde Sikiru; Selvaraju Sellappan; Backialakshmi Sekar; Ippala Janardhan Reddy; Raghavendra Bhatta
Volume 14, Issue 8 , August 2023, , Pages 405-413
Abstract
The effect of dietary calcium (Ca) and magnesium (Mg) supplementation on serum biochemical parameters, steroid hormones, gene expression, and the sex ratio was investigated in female New Zealand white rabbits. A total of 25 rabbits were allocated into five treatment groups: The control group was fed ...
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The effect of dietary calcium (Ca) and magnesium (Mg) supplementation on serum biochemical parameters, steroid hormones, gene expression, and the sex ratio was investigated in female New Zealand white rabbits. A total of 25 rabbits were allocated into five treatment groups: The control group was fed with regular pellet feed, whereas, treatment groups were supplemented with Ca and Mg: T1 (0.40% and 0.01%), T2 (0.60% and 0.02%), T3 (0.80% and 0.03%) and T4 (1.00% and 0.04%), respectively. The rabbits were subjected to three breeding cycles. The T3 group skewed towards females (65.33%) from all three breeding. There was elevated Ca concentration in T3 (15.26 ± 0.77 mg dL-1) and T4 (15.61 ± 0.82 mg dL-1) groups compared to the control. The concentration of estradiol was significantly high in T3 and T4 groups at 0.5 days post-coitus (dpc) and T2, T3 and T4 groups at 21dpc. Testosterone was significantly high in T4 group at 0.50 dpc and T2 and T4 group at 21dpc. The expression of 13 genes was studied in the oviduct. Genes such as OVGP1, CCT4, ANXA2 and TLR4 were up-regulated and positively correlated with the female sex ratio. The molecular functions and pathways of up-regulated genes were suggestive of their role in fertilization such as sperm selection, sperm storage, immune regulation, implantation and early embryonic development. The variations in the serum electrolytes, steroid hormones and gene expression might have an impact on the skewing process.
Zahra Salemi; Annahita Rezaie; Saad Goorani Nejad; Babak Mohammadian
Volume 11, Issue 4 , December 2020, , Pages 409-414
Abstract
This study aimed to determine cytological, histopathological and cytomorphometrical characteristics of endometrium in healthy and endometritic uterus in the water buffalo. Fifty eight non-pregnant reproductive systems were collected from slaughterhouse. Efficiency of three methods of sampling including ...
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This study aimed to determine cytological, histopathological and cytomorphometrical characteristics of endometrium in healthy and endometritic uterus in the water buffalo. Fifty eight non-pregnant reproductive systems were collected from slaughterhouse. Efficiency of three methods of sampling including cotton swab, smear, and aspiration were compared for cytologic study. Concurrent histopathologic examination revealed endometritis in 38 uteri including 8 (21.00%) with mild endometritis, 7 (18.42%) with moderate endometritis, 6 (15.90%) with severe endometritis and 17 (44.73%) with chronic endometritis. Cytomorphometrical results showed significant relationship between diameter and area of epithelial nuclei with phases of estrus cycle. Neutrophil and lymphocytes densities in swab and aspiration samples were significantly higher in severe endometritis than normal and chronic endometritis samples. Similarly, lymphocytes density in smear and aspiration methods was significant between normal and moderates, and also severe and chronic endometritis. Cytomorphometric analysis of epithelial nuclei characteristics (diameter and area) in buffalo were performed for the first time and it could be valuable to identify estrus cycle in this species. Aspiration had the most efficiency to identify endometritis in comparison with other methods.
Hossam Farouk Ahmed; Yamen Mohammed Hegazy; Sameh Abdelmageed Ibrahem
Volume 12, Issue 4 , December 2021, , Pages 409-414
Abstract
A total number of 62 clinically healthy dairy animals of three farms located in Kafr El Sheikh governorate, Egypt, were subjected to field screening surveys of subclinical mastitis (SCM) using California mastitis test (CMT). The obtained results revealed that 38.80% of quarter milk samples were positive ...
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A total number of 62 clinically healthy dairy animals of three farms located in Kafr El Sheikh governorate, Egypt, were subjected to field screening surveys of subclinical mastitis (SCM) using California mastitis test (CMT). The obtained results revealed that 38.80% of quarter milk samples were positive to CMT. The most frequently major causative agents isolated from the positive CMT samples were Escherichia coli, Staphylococcus aureus, and environmental streptococcus spp. Acute-phase proteins (APPs), as immunological biomarkers for SCM, including milk serum amyloid A (mSAA) and haptoglobin (Hp) were measured using ELISA. A significant positive correlation was found between the severity of the mammary infection of cow's quarter milk samples represented in somatic cell count (SCC) and each of APPs and pH values. The correlation coefficient (R) between SCC and mSAA, Hp and pH were 0.54, 0.38 and 0.73, respectively. On the other hand, there was a significant negative correlation between casein percentage in milk of SCM cases, and each of APPs, pH and the presence of bacterial pathogens in the milk samples. The obtained results threw light on the inter-relationship between SCC, mSAA, pH value and casein percentage in milk of cows and buffalo suffered from SCM. The percentage of casein in milk is considered a significant accurate tool for diagnosis of SCM and this finding offers the farmers a cheap and fast selection for diagnosis of such disease. These results presented a specific structured view on the efficacy of different diagnostic tools of SCM in dairy herds.
Farhad Tavarideh; Fazel Pourahmad; Mostafa Nemati
Volume 13, Issue 3 , September 2022, , Pages 409-415
Abstract
To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of Scrophularia striata was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of S. striata. Using sequence analysis targeting ...
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To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of Scrophularia striata was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of S. striata. Using sequence analysis targeting 16S rRNA gene, eight genera, including Agrococcus, Arthrobacter, Bacillus, Chryseobacterium, Delftia, Kocuria, Pseudomonas and Sphingomonas were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against Bacillus cereus, 17(54.84%) isolates exhibited maximum activity against Staphylococcus aureus, 14(45.16%) isolates against Escherichia coli and 5(16.13%) isolates showed positive activity against Proteus mirabilis.The results obtained in this study suggested that the medicinal plant, S. striatais is a potent source of endophytic bacteria with antibacterial activity and offers promise for discovery of more impressive biological compounds.
Sara Musaddiq; Mirza Imran Shahzad; Farzana Firdous; Atia Iqbal; Mehwish Tanveer; Abida Ashraf; Samina Aslam; Samia Khakwani
Volume 11, Issue 4 , December 2020, , Pages 415-421
Abstract
Viral outbreaks are a common cause of morbidity and mortality in livestock and human populations. Lack of good vaccines and poor control measures along with natural viral genetic drifting and shifting are the common causes of new viral strains and outbreaks. The current study reports the synthesis of ...
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Viral outbreaks are a common cause of morbidity and mortality in livestock and human populations. Lack of good vaccines and poor control measures along with natural viral genetic drifting and shifting are the common causes of new viral strains and outbreaks. The current study reports the synthesis of some 2-aryl substituted thiazolidine-4-carboxylic acids 1a-h and their 3-acetyl 2a and 3-benzoyl derivatives 3a. Two important poultry viruses: Avian influenza virus (AIV; A/Chicken/Italy/1994/H9N2) and infectious bronchitis virus (IBV) were selected, grown in 9-11 days old chicken embryonated eggs, and subjected to in ovo anti-viral assays. Most of the synthesized compounds were found active against AIV subtype H9N2 and IBV. In the case of AIV, the best results were attained for compound 1d which showed an IC50 value of 3.47 µM, while IBV 1c showed IC50 valueof4.10 µM. The lower IC50 values of these compounds correlate with the high potency of these compounds, especially in comparison with control groups. The standard drugs amantadine and ribavarin were used as positive controls in the case of AIV and IBV, respectively. Better results were obtained with 2-aryl substituted thiazolidine-4-carboxylic acids 1a-h compared to their N-acylated derivatives 2a and 3a against both viruses. In conclusion, this preliminary data support the idea that thiazolidine carboxylic acids could be used as anti-viral drugs against AIV and IBV infections.
Tahir Muhammad; Muhammad Ameen Jamal; Muhammad Ashraf; Nosheen Zafar; Shamaila Shahzadi; Tahir Maqbool; Faheem Hadi; Riaz Amjad
Volume 12, Issue 4 , December 2021, , Pages 415-420
Abstract
Currently, artificial oocyte activation has attracted wide attention in assisted reproduction due to extensive range of applications, particularly in somatic cell nuclear transfer and deriving pluripotent stem cell lines and it is the unique model to determine the role of paternal genome. Numbers of ...
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Currently, artificial oocyte activation has attracted wide attention in assisted reproduction due to extensive range of applications, particularly in somatic cell nuclear transfer and deriving pluripotent stem cell lines and it is the unique model to determine the role of paternal genome. Numbers of artificial activating agents have been used extensively to induce the oocytes activation; however, embryos developmental competency of artificially activated oocytes is still very low. In the present study, we determined the functional impact of strontium chloride supplementation with gold nanoparticles (AuNPs) in artificial oocytes activation and subsequent embryonic development. Oocytes were activated artificially in the culture medium containing 250 nM AuNPs with constant concentration of strontium chloride 10.00 mM. We found that adding 250 nM AuNPs with constant concentration of strontium chloride (10.00 mM for 3 hr) in culture medium improves the proportion of embryos reaching to the morula and blastocyst stages from 61.00% and 42.00% (controls) to 75.00% and 58.00% (250 nM AuNPs), respectively. In addition, foster mothers receiving AuNPs-treated embryos showed more implantation percentage and pregnancy rate relative to females received control embryos. Finally, embryos treated with 250 nM AuNPs concentration showed no toxic effect in term of blastocyst development. Collectively, our findings suggest the potential role of AuNPs in early embryonic development for mouse oocytes activated artificially and provide new insights in the field of animal biotechnology and assisted reproduction in humans.
Parasitology
Shivan Nawzad Hussein; Assel Anwer Ibrahim; Mohammed Shukri Shukur
Volume 14, Issue 8 , August 2023, , Pages 415-422
Abstract
This study aimed to identify Sarcocystis species isolated from macroscopic sarcocysts of naturally infected sheep and goats using histopathological and molecular studies. A total of 260 macrosarcocyst samples were randomly collected from 1,337 infected sheep and goats slaughtered at different abattoirs ...
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This study aimed to identify Sarcocystis species isolated from macroscopic sarcocysts of naturally infected sheep and goats using histopathological and molecular studies. A total of 260 macrosarcocyst samples were randomly collected from 1,337 infected sheep and goats slaughtered at different abattoirs in Duhok province, Iraq, from May 2021 to June 2022. The macroscopic cysts, which were found in the esophagus, diaphragm, and abdominal muscles, were classified into fat and thin cysts. Histopathological examination of the observed fat and thin cysts showed a thick eosinophilic wall, several internal septa-forming compartments enclosing numerous bradyzoites, and mild mononuclear inflammatory cells infiltrating around the cysts. The 18 Subunit ribosomal RNA (18S rRNA) and 28 Subunit ribosomal RNA (28S rRNA) genes of Sarcocystis spp. were amplified by polymerase chain reaction (PCR) from 200 macrosarcocysts samples. Molecularly, the DNA sequencing results obtained from fat macrocysts of sheep and goats were found to be identical to Sarcocystis gigantea, and from thin cysts of sheep proved to be similar to Sarcocystis medusiformis, while from thin macrocysts of goats were found to be identical to Sarcocystis moulei. Alignment and phylogenetic analysis observed a very close relationship between identified species of Sarcocystis and other Sarcocystis DNA sequences of sheep and goats across the world. To our knowledge, this is the first histopathological and molecular study for identification of Sarcocystis spp. isolated from different macroscopic forms of sarcocysts of sheep and goats in Iraq.
Akram Kholghi; Homeira Hatami; Nazli Khajehnasiri; Reihaneh Sadeghian
Volume 13, Issue 3 , September 2022, , Pages 417-422
Abstract
The effects of buprenorphine (BUP) on anxiety-like behavior and the expression of the glial fibrillary acidic protein (Gfap) and nuclear factor erythroid 2–related factor 2 (Nrf2) in methamphetamine (METH)-treated rats were investigated in this study. Twenty-eight male Wistar rats were randomly ...
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The effects of buprenorphine (BUP) on anxiety-like behavior and the expression of the glial fibrillary acidic protein (Gfap) and nuclear factor erythroid 2–related factor 2 (Nrf2) in methamphetamine (METH)-treated rats were investigated in this study. Twenty-eight male Wistar rats were randomly divided into four groups including control (saline), METH (10.00 mg kg-1), BUP (10.00 mg kg-1), and BUP + METH groups and treated for five days. On the final day of treatment, gene expression levels and anxiety were evaluated using elevated plus-maze (EPM). According to the results, five days of METH injection reduced open arm exploration in the EPM. In contrast, the open arm entries and the time spent in the open arms were increased in the BUP + METH group compared to the METH group. The expression levels of Gfap and Nrf2 were lower in METH-treated rats compared to controls, whereas Gfap and Nrf2 expression levels were higher in the METH+BUP-treated rats compared to the METH-treated rats, however, it was similar to the controls. These findings suggested that co-administration of BUP+METH could decrease anxiety-like behavior through increasing the activity of the antioxidant protection system and might have therapeutic potential for preventing anxiety in METH users.
Tuğçe Demirlek; Ahmet Sabuncu; Sinem Özlem Enginler; Baran Çelik; Ömür Koçak
Volume 12, Issue 4 , December 2021, , Pages 421-427
Abstract
Mastitis is the most important disease in dairy cattle industry because of its high economic losses both in herd management, milk and milk products. The aim of this study was to determine the efficacy of inactivated Parapoxvirus ovis (IPPVO) Para immune activator as a prophylaxis against mastitis and ...
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Mastitis is the most important disease in dairy cattle industry because of its high economic losses both in herd management, milk and milk products. The aim of this study was to determine the efficacy of inactivated Parapoxvirus ovis (IPPVO) Para immune activator as a prophylaxis against mastitis and as the therapy for subclinical mastitis in dairy cattle. The prophylactic effects of IPPVO were investigated in California mastitis test (CMT). Healthy Holstein cows were divided into A1 (n = 30) and A2 (n = 30) subgroups. In addition, 90 subclinical mastitis Holstein cows were divided into subgroups of B1, B2, and B3 to investigate the efficacy of IPPVO treatment. A significant difference in CMT levels was observed (p ˂ 0.01) 30 day after treatment in A groups. The difference in somatic cell count (SCC) levels between the A groups 15, 30 day after treatment was significant (p ˂ 0.01). The results of the CMT among the B groups showed no statistically significant difference (p > 0.05). The results of the SCC tests showed no statistically significant difference (p > 0.05) among the B groups on days 0, 9 and 15 after treatment. Coagulase-negative Staphylococcus (n = 53) and Escherichia coli (n = 30) were the most prevalent bacteria isolated in this study. In conclusion, IPPVO, although had no additional effect when used in combination with antibiotics could possibly be used instead of antibiotics and to protect cattle from subclinical mastitis, however, it is not known how long this prophylaxis effect could last.
Zakaria Iraninezhad; Mohammad Azizzadeh; Alireza Taghavi Razavizadeh; Jalil Mehrzad; Mohhamad Rashtibaf
Volume 11, Issue 4 , December 2020, , Pages 423-426
Abstract
Chlamydia abortus (C. abortus) is a Gram-negative intracellular bacteria responsible for major economic losses due mainly to infection and subsequent induction of abortion in several animal species and poses considerable public health problems in humans. This study was conducted to determine the prevalence ...
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Chlamydia abortus (C. abortus) is a Gram-negative intracellular bacteria responsible for major economic losses due mainly to infection and subsequent induction of abortion in several animal species and poses considerable public health problems in humans. This study was conducted to determine the prevalence of antibody against C. abortus in sheep and goat population of Khorasan Razavi province located in northeastern Iran. Four hundred fifty-two (271 sheep and 181 goats) sera samples from 40 sheep/goat epidemiologic units located in 11 counties were selected. Sera were assayed for antibodies against C. abortus using ELISA assay. Out of 452 sheep and goat sera, 44 [9.70% (95.00%CI: 7.10%-12.40%)] were positive for C. abortus antibodies. 28 out of 40 epidemiologic units (70.00%) and 10 out of 11 counties (91.00%), at least one seropositive sample was found. There was no significant difference between the seropositivity of sheep and goats. Age, sex, and location did not show significant relationship with the test results. The results showed that C. abortus was circulating in wide parts of Khorasan Razavi province. Considering the economic and public health importance of C. abortus, measures should be taken to help prevent its spread and to reduce the zoonotic risk of C. abortus in the studied region.
Hossein Khalesi; Mehdi Sakha; Abbas Veshkini; Ali Rezakhani
Volume 13, Issue 3 , September 2022, , Pages 423-429
Abstract
Heart murmurs and valvular regurgitation are common in horses and often have no effect on their performance. However, when structural changes occur in the heart size, they can affect performance adversely. This study aimed to examine the correlation between cardiac valves disease and poor performance ...
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Heart murmurs and valvular regurgitation are common in horses and often have no effect on their performance. However, when structural changes occur in the heart size, they can affect performance adversely. This study aimed to examine the correlation between cardiac valves disease and poor performance in athletic horses. A total of 300 athletic Thoroughbred and mix-breed horses including 164 Mares and 136 Stallions, with a history of poor performance, were selected. Horses with cardiac murmurs were identified and further cardiac examination including precise auscultation, base-apex electrocardiogram for possible dysrhythmias at rest and after exercise, echocardiographic and hematological tests were conducted in two stages. The first was at admission time and the second examination was done four to six months later to evaluate the outcome of the possible disorders. Respiratory system and musculoskeletal diseases were diagnosed respectively in 93 and 149 out of 300 examined horses and 36 horses showed heart murmur without any other complications. Echocardiography was performed in horses with heart murmur and 25 of them showed regurgitation of the cardiac valve. During the first examination, 7 horses were diagnosed with regurgitation and changes in the size of cardiac chambers, whereas this number increased to 25 during the second examination. There was no significant relationship between degree of murmur and severity of regurgitant jet in horses. The valvular regurgitation can affect the performance when causing changes in the size of the cardiac chambers which can consequently jeopardize the athletic future of the horse.
Immunology
Monireh Falsafi; Nowruz Delirezh; Elham Safarzadeh; Alireza Talebi; Zahra Heidari
Volume 14, Issue 8 , August 2023, , Pages 423-429
Abstract
Coccidiosis is the leading parasitic disease in poultry. One of the most critical Eimeria species, Eimeria tenella, lives inside the cecal epithelial cells and induces bloody coccidiosis. The present study evaluated the effect of radiation-attenuated E. tenella oocytes mixed with inulin adjuvant on broiler ...
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Coccidiosis is the leading parasitic disease in poultry. One of the most critical Eimeria species, Eimeria tenella, lives inside the cecal epithelial cells and induces bloody coccidiosis. The present study evaluated the effect of radiation-attenuated E. tenella oocytes mixed with inulin adjuvant on broiler chicken. Initially, the effect of irradiation on oocyst attenuation was confirmed. Then, one-day-old broilers (n = 90) were divided into nine groups on seven days of age as follow: Group 1 (400 attenuated oocysts + 1.00 mg of adjuvant), group 2 (400 attenuated oocysts + 0.50 mg adjuvant), group 3 (200 attenuated oocysts + 1.00 mg of adjuvant), group 4 (200 attenuated oocysts + 0.50 mg adjuvant), group 5 (1.00 mg adjuvant), group 6 (400 attenuated oocysts), group 7 (commercial vaccine), group 8 (negative control) and group 9 (blank). On day 21, we performed a challenge with E. tenella oocytes and investigated oocyst output and average weekly weight throughout the study. At the end of the study, we evaluated macroscopic lesion, histology, cytokine level and leukogram status. The results showed a statistically significant difference among groups. Furthermore, the optimal dose was 400 irradiated oocysts and 1.00 mg of inulin. Moreover, an X-ray could reduce the virulence of E. tenella oocytes. Inulin alone or combined with attenuated oocysts showed an acceptable effect on evaluated parameters.
Alireza Talebi; Amir Tukmechi; Ali-Asghar Tehrani; Sorayya Mahmudi
Volume 11, Issue 4 , December 2020, , Pages 427-429
Abstract
The black proventriculus is a necropsy finding sign observed during post-mortem examinations of dead young chicks up to 10 days of age and due to variation in color of the affected proventriculus organ, it may be ignored in some cases, in particular when the disease occurs simultaneously with other well-recognized ...
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The black proventriculus is a necropsy finding sign observed during post-mortem examinations of dead young chicks up to 10 days of age and due to variation in color of the affected proventriculus organ, it may be ignored in some cases, in particular when the disease occurs simultaneously with other well-recognized infections of two weeks old chicks. In late January 2018, several live and dead chicks with a history of an unusual increase in mortality showing no clinical signs but sudden dead were referred to the poultry clinic of Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. General routine postmortem examination revealed color changes (light green to dark green color) in the wall of proventriculus together with some degrees of congestion in the lungs and liver of the dead chicks. Microbiology tests revealed that the causative agent was Escherichia coli strain O142 and histopathological examination indicated that the color changing of the organ was due to necrosis of glandular epithelium with locally extensive or diffuse hypercellularity by lymphocytes and macrophages with hemorrhages. In conclusion, black proventriculus due to E. coli strain O142 was responsible for the sudden death of young chicks and based on antibiogram, selective antibiotics should be used for the treatment of the affected flocks.
Sara Salimi; Esmaeal Tamaddonfard; Farhad Soltanalinejad-Taghiabad
Volume 12, Issue 4 , December 2021, , Pages 429-436
Abstract
The aim of the present study was to investigate the effects of intra-ventrolateral periaqueductal gray (vlPAG) microinjection of histamine and thioperamide (a histamine H3 receptor antagonist/inverse agonist) on neuropathic pain. To explore the possible mechanism, naloxone was microinjected alone or ...
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The aim of the present study was to investigate the effects of intra-ventrolateral periaqueductal gray (vlPAG) microinjection of histamine and thioperamide (a histamine H3 receptor antagonist/inverse agonist) on neuropathic pain. To explore the possible mechanism, naloxone was microinjected alone or in combination with histamine and thioperamide. Neuropathic pain was induced by the left sciatic nerve chronic constriction injury. Both the right and left sides of vlPAG of the brain were surgically cannulated. Cold allodynia and mechanical hyperalgesia were recorded by acetone evaporation and von Frey filament tests. Areas under curve of allodynia and hyperalgesia were calculated. Histamine (0.50 and 2.00 µg per site), thioperamide (4.00 µg per site) and thioperamide (4.00 µg per site) before histamine (2.00 µg per site) suppressed cold allodynia and mechanical hyperalgesia after microinjection into the vlPAG. Microinjection of naloxone (0.25 and 1.00 µg per site) into the vlPAG had no effect on cold allodynia and mechanical hyperalgesia. The anti-allodynic and anti-hyperalgesic effects induced by microinjection of histamine (2.00 µg per site) and thioperamide (4.00 µg per site) into the vlPAG were inhibited by prior microinjection of naloxone (1.00 µg per site) into the same site. The above-mentioned agents did not alter locomotor activity. Based on our present results, it was concluded that exogenous (by histamine microinjection) and endogenous (by thioperamide microinjection) histamine of the vlPAG might contribute to the descending pain control mechanisms through a naloxone-sensitive mechanism.
