Shirzad Ghaderzadeh; Farzad Mirzaei Aghjehgheshlagh; Saeid Nikbin; Bahman Navidshad
Volume 11, Issue 4 , December 2020, , Pages 385-391
Abstract
Sheep keepers need suitable strategies to improve animal immunity and the quality of their products. This study was aimed to evaluate the effect of nano-selenium (nano-Se) and conjugated linoleic acid (CLA) on an antioxidant statue, trace minerals, and mRNA expression of glutathione peroxidase 1 (GPX1) ...
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Sheep keepers need suitable strategies to improve animal immunity and the quality of their products. This study was aimed to evaluate the effect of nano-selenium (nano-Se) and conjugated linoleic acid (CLA) on an antioxidant statue, trace minerals, and mRNA expression of glutathione peroxidase 1 (GPX1) and selenoprotein W1 (SEPW1) genes in the liver and peroxisome proliferator-activated receptor-gamma (PPARγ) and stearoyl COA desaturase 1 (SCD1) genes in fat- tail of male Moghani lambs. Thirty male Moghani lambs, three months old and average weight 30.00 ± 0.25 kg, were assigned to a completely randomized design in a 2×3 factorial arrangement with dietary supplementation of nano-Se (0, 1.00 and 2.00 mg kg-1 dry matter) and CLA (0 and 15.00 g kg-1 dry matter). The lambs were slaughtered at the end of the experiment, on day 90 of the experiment. Results showed that dietary inclusion of nano-Se significantly improved antioxidant enzymes glutathione peroxidase and superoxide dismutase in blood, however, did not show any differences in trace mineral treatments. The analysis of qPCR showed that nano-Se inclusion at the highest level (2.00 g kg-1 dry matter) enhanced gene expression of GPX1 (0.64 vs 0.34) and SEPW1 1 (0.72 vs 0.35) in the liver. Dietary inclusion of CLA increased the expression of PPARγ (0.63 vs 0.38) and decreased SCD1 (0.63 vs 0.33) genes in fat- tail. It could be concluded that selenium inclusion in the growing lamb’s diet could improve antioxidant status, however, no synergistic interaction was observed along with CLA on the mentioned parameters.
Clinical Pathology
Shahin Yadeghari; Mostafa Malecky; Mehdi Dehghan Banadaky; Bahman Navidshad
Volume 6, Issue 4 , December 2015, , Pages 285-293
Abstract
Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L(high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane ...
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Four in vitro experiments (Exp.) were conducted to evaluate lavender essential oil (LEO) effects at 0 (control), 250 (low dose), 500 (medium dose), 750 and 1000 µL per L(high doses) of incubation medium on rumen gas production kinetics (Exp.1), ruminal digestibility and fermentation (Exp.2), methane production (Exp.3) and rumen acidosis (Exp.4). The asymptote of gas production (A) increased quadratically (p < 0.001), but the lag phase (L) increased(p = 0.003), and gas production rate (µ) decreased linearly (p = 0.031) with increasing dose of LEO. A linear and quadratic effect (p < 0.01)was observed for the gas produced after 24 hr of incubation (GP24). In vitro true dry matter degradability (IVTDMD) and in vitro true organic matter degradability (IVTOMD) both decreased linearly (p < 0.01), but microbial biomass (MB) and partitioning factor (PF) changed quadratically with increasing doses of LEO (p < 0.05). A cubic effect was observed for total volatile fatty acid (TVFA) and ammonia (NH3) concentrations (p < 0.05). Acetate molar percentage decreased (p = 0.004), whereas those of butyrate and valerate increased linearly (p <0.05)with LEO dosage. The molar percentage of propionate increased by 10.60 and 12.00% at low and medium doses of LEO, respectively. Methane production decreased by 11.00 and 44.00 to 60.00% at medium and high doses of LEO (p < 0.05), respectively. Lavender essential oil decreased also ruminal pH at all included doses (p < 0.05), intensifying rumen acidosis. These results revealed a dose-dependent selective effect (stimulatory at low and medium, and inhibitory at high doses) of LEO on rumen fermentation.
