Pathology
Emin Karakurt; Nuvit Coskun; Uğur Aydın; Serpil Dağ; Enver Beytut; Veysel Soydal Ataseven; Volkan Yılmaz; Fırat Doğan; Hilmi Nuhoğlu; Celal Şahin Ermutlu; Ayfer Yıldız
Articles in Press, Accepted Manuscript, Available Online from 02 December 2023
Abstract
This study was aimed at the evaluation of cell proliferation, the p53 tumor-suppressor gene and the apoptosis index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs, which were admitted to the Pathology Department. ...
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This study was aimed at the evaluation of cell proliferation, the p53 tumor-suppressor gene and the apoptosis index by immunohistochemical methods in canine oral papillomatosis. The study material comprised of tumor tissue samples taken from six dogs, which were admitted to the Pathology Department. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Cases of canine oral papillomatosis, determined to have been caused by CPV-1, were found to have a rather high cell proliferation index, Proliferating Cell Nucleus Antigen (PCNA). Furthermore, all cases were immunohistochemically demonstrated to carry a mutant p53 gene. Despite the mutation of the p53 gene, the shift in the Bax/Bcl-2 ratio of the dogs diagnosed with tumor was in favor of the proapoptotic Bax gene. The apoptotic mechanism was determined to occur through both the caspase-dependent and caspase-independent pathways. While the lesions occupied the entire oral cavity in some cases, histopathologically, malignant transformation was not detected in any of the 6 cases.
Emin Karakurt; Nüvit Coşkun; Enver Beytut; Ömer Faruk Keleş; Serpil Dağ; Volkan Yilmaz; Hilmi Nuhoğlu; Ayfer Yıldız; Emre Kurtbaş
Volume 14, Issue 1 , January 2023, , Pages 1-6
Abstract
In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine ...
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In this study, it was aimed to investigate the association between inflammatory reaction of tumoral microenvironments with interleukin responses in ovine pulmonary adenocarcinomas (OPAs). Material of the study consisted of 26 sheep lung tissue samples being brought to the Pathology Department for routine diagnosis. Cases were collected between years 2009 - 2021; pre-diagnosis was based on clinical symptoms, anamnesis and gross lesion of the lungs. These tissues were designated in two groups as control (n = 6) and OPA (n = 20) groups. Choice of immunohistochemical staining was avidin-biotin peroxidase method. Reverse transcription polymerase chain reaction (RT-PCR) was used to confirm Jaagsiekte sheep retrovirus from paraffin-embedded tissues. On gross examination of OPAs, lesions seen were mostly in the caudal lobes of the lung, 1.00 – 2.00 cm in diameter as gray-white consolidated foci and in microscopic observation, tumor cells showed acinar, papillary or mixed growths. No expressions of interleukin (2 and 8) were observed in the control group. All OPAs cases were positive for interleukins (2 and 8) expressions. A total of eight tissue samples were detected as positives through RT-PCR. In conclusion, in this study, it was determined that interleukin-2 and interleukin-8 were produced from tumor microenvironment elements, especially tumor-associated macrophages, and these interleukins showed pro-inflammatory effects. Interleukins and the inflammatory reaction may promote the development of OPA.
