Document Type : Original Article

Authors

• Reza Narenji Sani ¹
• Parviz Tajik ¹
• Mohammad Hassan yousefi ²
• Mansoureh Movahedin ³
• Babak Qasemi-Panahi ⁴
• Shiva Shafiei ¹
• Mahmood Ahmadi Hamedani ²

¹ Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

² Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

³ Department of Anatomical Sciences, School of Medical Sciences, Tarbiat Modarres University, Tehran, Iran

⁴ Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells (SCCs) have provided very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules is consist of spermatogonial stem cells. To investigate and biomanipulation of these cells, proliferation and viability rate of cells should be increased in vitro, at first. Follicle stimulating hormone (FSH) has been suggested to play a determinant role in the survival of germ cells in addition to increasing spermatogonial proliferation. In this study, the in vitro effects of FSH on spermatogonial cell colony formation were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. The identity of the Sertoli cells and spermatogonial stem cells were confirmed through immunocytochemistry and colony morphology, respectively. Co-cultured Sertoli and spermatogonial cells were treated with FSH in different dose of 10, 20 and 40 IU mL^-1 FSH, before colony assay. Results indicated that, FSH increased in vitro colonization of spermatogonial cells in comparison with control group. In conclusion, using FSH provided proper bovine spermatogonial stem cell culture medium for in vitro study of these cells.

Keywords

• Bovine
• Co-culture
• FSH
• Sertoli
• SSCs