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Differences between some biochemical components in seminal plasma of ‎first and second ejaculations in dual-purpose Simmental (Fleckvieh) bulls ‎and their relationships with semen quality parameters

Document Type : Original Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

2 Iran Simmental Cattle Breeding Center, Amard-Dam Company, Amol, Iran

Abstract

This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in Fleckvieh bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first and second ejaculations. All frozen-thawed semen CASA parameters (except lateral head displacement) were associated with fresh motility parameters and before and after thawing sperm viability. Also, a correlation between seminal Zn concentration with fresh semen gross and progressive motility, average path velocity, and beat cross frequency, Cu with SOD and Fe and semen concentration was observed. CAT was associated with fresh and frozen-thawed sperm motility parameters except for lateral head displacement and angular displacement. Although our findings showed differences between the first and second ejaculations in some parameters, PMFT, which is the most important indicator for estimating bull fertility, was not different between them.

Keywords

References
  1. Parkinson T. Normal reproduction in male animals. In: Noakes DE, Parkinson TJ, England GCW (Eds). Veterinary Reproduction and Obstetrics. 9th ed. London, UK: Saunders 2009; 696.
  2. Bergeron A, Manjunath P. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol Reprod Dev 2006; 73(10): 1338-1344.
  3. Vince S, Žura Žaja I, Samardžija M, et al. Age-related differences of semen quality, seminal plasma, and spermatozoa antioxidative and oxidative stress variables in bulls during cold and warm periods of the year. Animal 2018; 12(3): 559-568.
  4. Colagar AH, Marzony ET, Chaichi, MJ. Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutr Res 2009; 29(2): 82-88.
  5. Roy B, Nagpaul PK. Influence of Zn supplementation (organic and inorganic) on blood testosterone profile of Murrah buffalo bulls. Buffalo Bull 2017; 36(3): 525-530.
  6. Wong WY, Merkus HMWM, Thomas CMG, et al. Effects of folic acid and zinc sulfate on male factor subfertility: a double-blind, randomized, placebo-controlled trial. Fertil Steril 2002; 77(3): 491-498.
  7. Saaranen M, Suistomaa U, Kantola M, et al. Lead, magnesium, selenium and zinc in human seminal fluid: comparison with semen parameters and fertility. Hum Reprod 1987; 2(6): 475-479.
  8. Qureshi IZ, Abbas Q. Modulation of testicular and whole blood trace element concentrations in conjunction with testosterone release following kisspeptin administration in male rabbits (Oryctolagus cuniculus). Biol Trace Elem Res 2013; 154(2): 210-216.
  9. Liu JY, Yang X, Sun XD, et al. Suppressive effects of copper sulfate accumulation on the spermatogenesis of rats. Biol Trace Elem Res 2016; 174(2): 356-361.
  10. Tvrdá E, Kňažická Z, Lukáčová J, et al. Relationships between iron and copper content, motility characteristics and antioxidant status in bovine seminal plasma. J Microbiol Biotechnol Food Sci 2012; 2(2): 536-547.
  11. Anchordoquy JP, Anchordoquy JM, Pascua AM, et al. The copper transporter (SLC31A1/CTR1) is expressed in bovine spermatozoa and oocytes: Copper in IVF medium improves sperm quality. Theriogenology 2017; 97: 124-133.
  12. Narasimhaiah M, Arunachalam A, Sellappan S, et al. Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats. Reprod Domest Anim 2018; 53(3): 644-654.
  13. Aydemir B, Kiziler AR, Onaran I, et al. Impact of Cu and Fe concentrations on oxidative damage in male infertility. Biol Trace Elem Res 2006; 112(3): 193-203.
  14. Kanwal MR, Rehman NU, Ahmad N, et al. Bulk cations and trace elements in the Nili-Ravi buffalo and crossbred cow bull semen. Int J Agric Biol 2000; 2(4): 302-305.
  15. Tvrda E, Peer R, Sikka SC, et al. Iron and copper in male reproduction: a double-edged sword. J Assist Reprod Genet 2015; 32(1): 3-16.
  16. Tvrdá E, Lukáč N, Schneidgenová M, et al. Impact of seminal chemical elements on the oxidative balance in bovine seminal plasma and spermatozoa. J Vet Med 2013; 2013: 125096. doi: 10.1155/2013/125096.
  17. Kadirve G, Kumar S, Ghosh SK, et al. Activity of antioxidative enzymes in fresh and frozen thawed buffalo (Bubalus bubalis) spermatozoa in relation to lipid peroxidation and semen quality. Asian Pac J Reprod 2014; 3(3): 210-217.
  18. Nenkova G, Petrov L, Alexandrova A. Role of trace elements for oxidative status and quality of human sperm. Balkan Med J 2017; 34(4): 343-348.
  19. Waheed MM, Gouda EM, Khalifa TAA. Impact of seminal plasma superoxide dismutase and glutathione peroxidase on cryopreserved buffalo spermatozoa. Anim Reprod Sci 2013; 142(3-4): 126-130.
  20. Gürler H, Calisici O, Bollwein H. Inter- and intra-individual variability of total antioxidant capacity of bovine seminal plasma and relationships with sperm quality before and after cryopreservation. Anim Reprod Sci 2015; 155: 99-105.
  21. Li Y, Kalo D, Zeron Y, et al. Progressive motility - a potential predictive parameter for semen fertilization capacity in bovines. Zygote 2016; 24(1): 70-82.
  22. Henkel R, Bittner J, Weber R, et al. Relevance of zinc in human sperm flagella and its relation to motility. Fertil Steril 1999; 71(6): 1138-1143.
  23. Pesch S, Bergmann M, Bostedt H. Determination of some enzymes and macro- and microelements in stallion seminal plasma and their correlations to semen quality. Theriogenology 2006; 66(2): 307-313.
  24. Barrier-Battut I, Delajarraud H, Legrand E, et al. Calcium, magnesium, copper, and zinc in seminal plasma of fertile stallions, and their relationship with semen freezability. Theriogenology 2002; 58(2-4): 229-232.
  25. Usuga A, Rojano B, Restrepo G. Effect of seminal plasma components on the quality of fresh and cryopreserved stallion semen. J Equine Vet Sci 2017; 58: 103-111.
  26. Kumar N, Verma RP, Singh LP, et al. Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus x Bos taurus) bulls. Reprod Nutr Dev 2006; 46(6): 663-675.
  27. Alavi-Shoushtari SM, Asri-Rezai S, Ansari MHKh, et al. Effects of the seminal plasma zinc content and catalase activity on the semen quality of water buffalo (Bubalus bubalis) bulls. Pak J Biol Sci 2009; 12(2): 134-139.
  28. Chia SE, Ong CN, Chua, LH, et al. Comparison of zinc concentrations in blood and seminal plasma and the various sperm parameters between fertile and infertile men. J Androl 2000; 21(1): 53-57.
  29. Sørensen MB, Bergdahl IA, Hjøllund NH, et al. Zinc, magnesium and calcium in human seminal fluid: relations to other semen parameters and fertility. Mol Hum Reprod 1999; 5(4): 331-337.
  30. Kirton KT, Hafs HD, Hunter AG. Levels of some normal constituents of bull semen during repetitive ejaculation. J Reprod Fertil 1964; 8: 157-164.
  31. Valsa J, Gusani PH, Skandhan KP, et al. Copper in split and daily ejaculates. J Reprod Med 1994; 39(9): 725-728.
  32. Máchal L, Chládek G, Straková E. Copper, phosphorus and calcium in bovine blood and seminal plasma in relation to semen quality. J Anim Feed Sci 2002; 11(3): 425-435.
  33. Akalın PP, Bülbül B, Çoyan K, et al. Relationship of blood and seminal plasma ceruloplasmin, copper, iron and cadmium concentrations with sperm quality in Merino rams. Small Ruminant Res 2015; 133: 135-139.
  34. Sun J, Yu G, Zhang Y, et al. Heavy metal level in human semen with different fertility: a meta-analysis. Biol Trace Elem Res 2017; 176(1): 27-36.
  35. Hashemi MM, Behnampour N, Nejabat M, et al. Impact of seminal plasma trace elements on human sperm motility parameters. Rom J Intern Med 2018; (56): 15-20.
  36. Marzec-Wróblewska U, Kamiński P, Łakota P, et al. The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality. Reprod Fertil Dev 2015; 28(10): 1518-1525.
  37. Ghaniei A, Eslami M, BabaeiMarzango SS et al. Determination of calcium, magnesium, phosphorus, iron, and copper contents in rooster seminal plasma and their effects on semen quality. Comp Clin Pathol 2018; 27: 427-431.
  38. Eghbali M, Alavi-Shoushtari SM, Asri-Rezaei S, et al. Effects of the seminal plasma iron and lead content on semen quality of Water Buffalo (Bubalus bubalis) bulls. Vet Res Forum 2010; 1(3): 142-148.
  39. Massányi P, Trandzik J, Nad P, et al. Concentration of copper, iron, zinc, cadmium, lead, and nickel in bull and ram semen and relation to the occurrence of patho-logical spermatozoa. J Environ Sci Health A Tox Hazard Subst Environ Eng 2004; 39(11-12): 3005-3014.
  40. Marzec-Wróblewska U, Kamiński P, Lakota P, et al. Zinc and iron concentration and SOD activity in human semen and seminal plasma. Biol Trace Elem Res 2011; 143(1): 167-177.
  41. Abou-Shakra FR, Ward NI, Everard DM. The role of trace elements in male infertility. Fertil Steril 1989; 52(2): 307-310.
  42. Khosrowbeygi A, Zarghami N. Levels of oxidative stress biomarkers in seminal plasma and their relationship with seminal parameters. BMC Clin Pathol 2007; 7: 6. doi:10.1186/1472-6890-7-6.
  43. Siciliano L, Tarantino P, Longobardi F, et al. Impaired seminal antioxidant capacity in human semen with hyperviscosity or oligoasthenozoospermia. J Androl 2001; 22(5): 798-803.
  44. Hsieh YY, Sun YL, Chang CC, et al. Superoxide dismutase activities of spermatozoa and seminal plasma are not correlated with male infertility. J Clin Lab Anal 2002; 16(3): 127-131.
  45. Eghbali M, Alavi-Shoushtari SM, Asri Rezaii S. Effects of copper and superoxide dismutase content of seminal plasma on buffalo semen characteristics. Pak J Biol Sci 2008; 11(15): 1964-1968.
  46. Zini A, Garrels K, Phang D. Antioxidant activity in the semen of fertile and infertile men. Urology 2000; 55(6): 922-926.
  47. Neagu VR, Macías García B, Morillo Rodríguez A, et al. Determination of glutation peroxidase and superoxide dismutase activities in canine seminal plasma and its relation with sperm quality and lipid peroxidation post thaw. Theriogenology 2011; 75(1): 10-16.
  48. Hsieh YY, Chang CC, Lin CS. Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int J Biol Sci 2006; 2(1): 23-29.
  49. Kasimanickam R, Kasimanickam V, Thatcher CD, et al. Relationships among lipid peroxidation, glutathione peroxidase, superoxide dismutase, sperm parameters, and competitive index in dairy bulls. Theriogenology 2007; 67(5): 1004-1012.
  50. Baumber J, Ball BA. Determination of glutathione peroxidase and superoxide dismutase-like activities in equine spermatozoa, seminal plasma, and reproductive tissues. Am J Vet Res 2005; 66(8): 1415-1419.
Veterinary Research Forum
Volume 12, Issue 1
March 2021
Pages 39-46
Files
History
  • Receive Date: 01 December 2018
  • Revise Date: 02 March 2019
  • Accept Date: 15 April 2019
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