Veterinary Research Forum
Vet Res Forum

Differences between some biochemical components in seminal plasma of ‎first and second ejaculations in dual-purpose Simmental (Fleckvieh) bulls ‎and their relationships with semen quality parameters

Document Type : Original Article

Authors

Amir Khaki 1, 2
Atefeh Araghi 1
Mehdi Lotfi 2
Alireza Nourian 2

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

2 Iran Simmental Cattle Breeding Center, Amard-Dam Company, Amol, Iran

https://doi.org/10.30466/vrf.2019.98781.2355
Abstract
This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in Fleckvieh bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first and second ejaculations. All frozen-thawed semen CASA parameters (except lateral head displacement) were associated with fresh motility parameters and before and after thawing sperm viability. Also, a correlation between seminal Zn concentration with fresh semen gross and progressive motility, average path velocity, and beat cross frequency, Cu with SOD and Fe and semen concentration was observed. CAT was associated with fresh and frozen-thawed sperm motility parameters except for lateral head displacement and angular displacement. Although our findings showed differences between the first and second ejaculations in some parameters, PMFT, which is the most important indicator for estimating bull fertility, was not different between them.

Keywords

‎ Cattle repetitive ejaculations
‎ Enzymes
‎ Micro minerals
‎ Seminal plasma
‎ Semen quality parameters
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Veterinary Research Forum
Volume 12, Issue 1
Winter 2021
Pages 39-46

  • Receive Date 01 December 2018
  • Revise Date 02 March 2019
  • Accept Date 15 April 2019

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