Veterinary Research Forum
Vet Res Forum

Molecular effects of curcumin on the experimental autoimmune ‎encephalomyelitis

Document Type : Original Article

Authors

Laleh Mavaddatiyan 1
Shiva Khezri 1
seyyed Meysam Abtahi Froushani 2

1 Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran

2 Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2019.98789.2356
Abstract
Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS). Previous studies have shown that myelin degradation during MS and EAE resulted in reduced expression of some of the proteins, e.g., the MBP (myelin basic protein), and increased expression of genes such as iNOS (Inducible nitric oxide synthase) and NOGO-A in the affected patients. In the present study, EAE was induced by immunizing Wistar rats (n=12) with homogenized spinal cord of guinea pig and Freund's complete adjuvant. Curcumin is an active ingredient in turmeric with anti-inflammatory properties, which has been studied in this article. In this study, the effect of curcumin administration on the change of the expression of MBP, NOGO-A, and iNOS genes was evaluated using the RT-PCR (Reverse transcription-polymerase chain reaction) technique. The obtained results indicated it could be concluded that curcumin was able to improve EAE by increasing the amount of MBP gene expression and reducing the intensity of NOGO-A expression.

Keywords

Curcumin
‎ Encephalomyelitis
‎ Multiple sclerosis
‎ RT PCR
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Veterinary Research Forum
Volume 12, Issue 1
Winter 2021
Pages 47-52

  • Receive Date 03 December 2018
  • Revise Date 22 January 2019
  • Accept Date 02 February 2019

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